Microglia Heterogeneity in the Single-Cell Era
Microglia are resident immune cells in the central nervous system (CNS) that are capable of carrying out prominent and various functions during development and adulthood under both homeostatic and disease conditions. Although microglia are traditionally thought to be heterogeneous populations, which...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472030019X |
| _version_ | 1819230168859803648 |
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| author | Takahiro Masuda Roman Sankowski Ori Staszewski Marco Prinz |
| author_facet | Takahiro Masuda Roman Sankowski Ori Staszewski Marco Prinz |
| author_sort | Takahiro Masuda |
| collection | DOAJ |
| description | Microglia are resident immune cells in the central nervous system (CNS) that are capable of carrying out prominent and various functions during development and adulthood under both homeostatic and disease conditions. Although microglia are traditionally thought to be heterogeneous populations, which potentially allows them to achieve a wide range of responses to environmental changes for the maintenance of CNS homeostasis, a lack of unbiased and high-throughput methods to assess microglia heterogeneity has prevented the study of spatially and temporally distributed microglia subsets. The recent emergence of novel single-cell techniques, such as cytometry by time-of-flight mass spectrometry (CyTOF) and single-cell RNA sequencing, enabled scientists to overcome such limitations and reveal the surprising context-dependent heterogeneity of microglia. In this review, we summarize the current knowledge about the spatial, temporal, and functional diversity of microglia during development, homeostasis, and disease in mice and humans. : In this review, Masuda et al. summarize the current knowledge, open questions, and future directions regarding the spatial, temporal, and functional multiplicity of microglia during development, homeostasis, and disease in both mice and humans. Keywords: microglia, heterogeneity, CNS, macrophages, single-cell RNA sequencing, CyTOF, human, mouse |
| first_indexed | 2024-12-23T11:24:47Z |
| format | Article |
| id | doaj.art-6db3c8b1320b4db996f458e7c79e066e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T11:24:47Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-6db3c8b1320b4db996f458e7c79e066e2022-12-21T17:48:59ZengElsevierCell Reports2211-12472020-02-0130512711281Microglia Heterogeneity in the Single-Cell EraTakahiro Masuda0Roman Sankowski1Ori Staszewski2Marco Prinz3Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, GermanyInstitute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; Berta-Ottenstein-Programme for Clinician Scientists, Faculty of Medicine, University of Freiburg, Freiburg, GermanyInstitute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; Berta-Ottenstein-Programme for Clinician Scientists, Faculty of Medicine, University of Freiburg, Freiburg, GermanyInstitute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany; Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, Freiburg, Germany; Corresponding authorMicroglia are resident immune cells in the central nervous system (CNS) that are capable of carrying out prominent and various functions during development and adulthood under both homeostatic and disease conditions. Although microglia are traditionally thought to be heterogeneous populations, which potentially allows them to achieve a wide range of responses to environmental changes for the maintenance of CNS homeostasis, a lack of unbiased and high-throughput methods to assess microglia heterogeneity has prevented the study of spatially and temporally distributed microglia subsets. The recent emergence of novel single-cell techniques, such as cytometry by time-of-flight mass spectrometry (CyTOF) and single-cell RNA sequencing, enabled scientists to overcome such limitations and reveal the surprising context-dependent heterogeneity of microglia. In this review, we summarize the current knowledge about the spatial, temporal, and functional diversity of microglia during development, homeostasis, and disease in mice and humans. : In this review, Masuda et al. summarize the current knowledge, open questions, and future directions regarding the spatial, temporal, and functional multiplicity of microglia during development, homeostasis, and disease in both mice and humans. Keywords: microglia, heterogeneity, CNS, macrophages, single-cell RNA sequencing, CyTOF, human, mousehttp://www.sciencedirect.com/science/article/pii/S221112472030019X |
| spellingShingle | Takahiro Masuda Roman Sankowski Ori Staszewski Marco Prinz Microglia Heterogeneity in the Single-Cell Era Cell Reports |
| title | Microglia Heterogeneity in the Single-Cell Era |
| title_full | Microglia Heterogeneity in the Single-Cell Era |
| title_fullStr | Microglia Heterogeneity in the Single-Cell Era |
| title_full_unstemmed | Microglia Heterogeneity in the Single-Cell Era |
| title_short | Microglia Heterogeneity in the Single-Cell Era |
| title_sort | microglia heterogeneity in the single cell era |
| url | http://www.sciencedirect.com/science/article/pii/S221112472030019X |
| work_keys_str_mv | AT takahiromasuda microgliaheterogeneityinthesinglecellera AT romansankowski microgliaheterogeneityinthesinglecellera AT oristaszewski microgliaheterogeneityinthesinglecellera AT marcoprinz microgliaheterogeneityinthesinglecellera |