Replication origin location might contribute to genetic variability in Trypanosoma cruzi
Abstract Background DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among stra...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-020-06803-8 |
| _version_ | 1819048080350117888 |
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| author | Christiane Bezerra de Araujo Julia Pinheiro Chagas da Cunha Davi Toshio Inada Jeziel Damasceno Alex Ranieri Jerônimo Lima Priscila Hiraiwa Catarina Marques Evonnildo Gonçalves Milton Yutaka Nishiyama-Junior Richard McCulloch Maria Carolina Elias |
| author_facet | Christiane Bezerra de Araujo Julia Pinheiro Chagas da Cunha Davi Toshio Inada Jeziel Damasceno Alex Ranieri Jerônimo Lima Priscila Hiraiwa Catarina Marques Evonnildo Gonçalves Milton Yutaka Nishiyama-Junior Richard McCulloch Maria Carolina Elias |
| author_sort | Christiane Bezerra de Araujo |
| collection | DOAJ |
| description | Abstract Background DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown. Results Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1). Conclusions These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. |
| first_indexed | 2024-12-21T11:10:34Z |
| format | Article |
| id | doaj.art-6dbc6fe9785f4d69b02a1412dde1e24e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T11:10:34Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-6dbc6fe9785f4d69b02a1412dde1e24e2022-12-21T19:06:06ZengBMCBMC Genomics1471-21642020-06-0121111610.1186/s12864-020-06803-8Replication origin location might contribute to genetic variability in Trypanosoma cruziChristiane Bezerra de Araujo0Julia Pinheiro Chagas da Cunha1Davi Toshio Inada2Jeziel Damasceno3Alex Ranieri Jerônimo Lima4Priscila Hiraiwa5Catarina Marques6Evonnildo Gonçalves7Milton Yutaka Nishiyama-Junior8Richard McCulloch9Maria Carolina Elias10Laboratório de Ciclo Celular, Instituto ButantanLaboratório de Ciclo Celular, Instituto ButantanLaboratório de Ciclo Celular, Instituto ButantanThe Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of GlasgowLaboratório de Tecnologia Biomolecular – Bioinformática, Instituto de Ciências Biológicas, Universidade Federal do ParáFundação Oswaldo Cruz, Instituto Carlos ChagasThe Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of GlasgowLaboratório de Tecnologia Biomolecular – Bioinformática, Instituto de Ciências Biológicas, Universidade Federal do ParáCenter of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto ButantanThe Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of GlasgowLaboratório de Ciclo Celular, Instituto ButantanAbstract Background DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown. Results Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1). Conclusions These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins.http://link.springer.com/article/10.1186/s12864-020-06803-8Replication originsTrypanosoma cruziGenetic variabilityDGF-1 |
| spellingShingle | Christiane Bezerra de Araujo Julia Pinheiro Chagas da Cunha Davi Toshio Inada Jeziel Damasceno Alex Ranieri Jerônimo Lima Priscila Hiraiwa Catarina Marques Evonnildo Gonçalves Milton Yutaka Nishiyama-Junior Richard McCulloch Maria Carolina Elias Replication origin location might contribute to genetic variability in Trypanosoma cruzi BMC Genomics Replication origins Trypanosoma cruzi Genetic variability DGF-1 |
| title | Replication origin location might contribute to genetic variability in Trypanosoma cruzi |
| title_full | Replication origin location might contribute to genetic variability in Trypanosoma cruzi |
| title_fullStr | Replication origin location might contribute to genetic variability in Trypanosoma cruzi |
| title_full_unstemmed | Replication origin location might contribute to genetic variability in Trypanosoma cruzi |
| title_short | Replication origin location might contribute to genetic variability in Trypanosoma cruzi |
| title_sort | replication origin location might contribute to genetic variability in trypanosoma cruzi |
| topic | Replication origins Trypanosoma cruzi Genetic variability DGF-1 |
| url | http://link.springer.com/article/10.1186/s12864-020-06803-8 |
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