Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process

This manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absor...

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Main Authors: Jan Zidek, Anna Sudakova, Jiri Smilek, Duc Anh Nguyen, Hung Le Ngoc, Le Minh Ha
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Gels
Subjects:
Online Access:https://www.mdpi.com/2310-2861/9/11/888
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author Jan Zidek
Anna Sudakova
Jiri Smilek
Duc Anh Nguyen
Hung Le Ngoc
Le Minh Ha
author_facet Jan Zidek
Anna Sudakova
Jiri Smilek
Duc Anh Nguyen
Hung Le Ngoc
Le Minh Ha
author_sort Jan Zidek
collection DOAJ
description This manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absorption characteristics. Image analysis serves as a tool for examining dye diffusion and absorption. The results indicate a correlation between dye concentrations and membrane thickness. Thinner layers exhibit a consistent dye concentration, implying an even distribution of the dye during the diffusion process. However, thicker layers display varying concentrations at different edges, suggesting the establishment of a diffusion gradient. Moreover, the authors observe an increased concentration of gelatin at the peripheries rather than at the center, possibly due to the swelling of the dried sample and a potential water concentration gradient. The manuscript concludes by suggesting image analysis as a practical alternative to spectral analysis, particularly for detecting whether methylene blue has been adsorbed onto the macromolecular network. These findings significantly enhance the understanding of the complex interactions between methylene blue and gelatin in a membrane and lay a solid foundation for future research in this field.
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spelling doaj.art-6dbfef966e6d40f18e68678e8d1ea6212023-11-24T14:43:24ZengMDPI AGGels2310-28612023-11-0191188810.3390/gels9110888Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion ProcessJan Zidek0Anna Sudakova1Jiri Smilek2Duc Anh Nguyen3Hung Le Ngoc4Le Minh Ha5Central European Institute of Technology (CEITEC), Brno University of Technology, Purkynova 123, 612 00 Brno, Czech RepublicCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkynova 123, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 464/118, 612 00 Brno, Czech RepublicCenter for Research and Technology Transfer (CRETECH), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamCenter for Research and Technology Transfer (CRETECH), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamInstitute of Natural Products Chemistry (INPC), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamThis manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absorption characteristics. Image analysis serves as a tool for examining dye diffusion and absorption. The results indicate a correlation between dye concentrations and membrane thickness. Thinner layers exhibit a consistent dye concentration, implying an even distribution of the dye during the diffusion process. However, thicker layers display varying concentrations at different edges, suggesting the establishment of a diffusion gradient. Moreover, the authors observe an increased concentration of gelatin at the peripheries rather than at the center, possibly due to the swelling of the dried sample and a potential water concentration gradient. The manuscript concludes by suggesting image analysis as a practical alternative to spectral analysis, particularly for detecting whether methylene blue has been adsorbed onto the macromolecular network. These findings significantly enhance the understanding of the complex interactions between methylene blue and gelatin in a membrane and lay a solid foundation for future research in this field.https://www.mdpi.com/2310-2861/9/11/888methylene bluegelatinimage analysisdye bindingresonance stateslayered materials
spellingShingle Jan Zidek
Anna Sudakova
Jiri Smilek
Duc Anh Nguyen
Hung Le Ngoc
Le Minh Ha
Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
Gels
methylene blue
gelatin
image analysis
dye binding
resonance states
layered materials
title Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
title_full Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
title_fullStr Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
title_full_unstemmed Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
title_short Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
title_sort explorative image analysis of methylene blue interactions with gelatin in polypropylene nonwoven fabric membranes a potential future tool for the characterization of the diffusion process
topic methylene blue
gelatin
image analysis
dye binding
resonance states
layered materials
url https://www.mdpi.com/2310-2861/9/11/888
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