Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process
This manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absor...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2023-11-01
|
Series: | Gels |
Subjects: | |
Online Access: | https://www.mdpi.com/2310-2861/9/11/888 |
_version_ | 1797459193908690944 |
---|---|
author | Jan Zidek Anna Sudakova Jiri Smilek Duc Anh Nguyen Hung Le Ngoc Le Minh Ha |
author_facet | Jan Zidek Anna Sudakova Jiri Smilek Duc Anh Nguyen Hung Le Ngoc Le Minh Ha |
author_sort | Jan Zidek |
collection | DOAJ |
description | This manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absorption characteristics. Image analysis serves as a tool for examining dye diffusion and absorption. The results indicate a correlation between dye concentrations and membrane thickness. Thinner layers exhibit a consistent dye concentration, implying an even distribution of the dye during the diffusion process. However, thicker layers display varying concentrations at different edges, suggesting the establishment of a diffusion gradient. Moreover, the authors observe an increased concentration of gelatin at the peripheries rather than at the center, possibly due to the swelling of the dried sample and a potential water concentration gradient. The manuscript concludes by suggesting image analysis as a practical alternative to spectral analysis, particularly for detecting whether methylene blue has been adsorbed onto the macromolecular network. These findings significantly enhance the understanding of the complex interactions between methylene blue and gelatin in a membrane and lay a solid foundation for future research in this field. |
first_indexed | 2024-03-09T16:48:54Z |
format | Article |
id | doaj.art-6dbfef966e6d40f18e68678e8d1ea621 |
institution | Directory Open Access Journal |
issn | 2310-2861 |
language | English |
last_indexed | 2024-03-09T16:48:54Z |
publishDate | 2023-11-01 |
publisher | MDPI AG |
record_format | Article |
series | Gels |
spelling | doaj.art-6dbfef966e6d40f18e68678e8d1ea6212023-11-24T14:43:24ZengMDPI AGGels2310-28612023-11-0191188810.3390/gels9110888Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion ProcessJan Zidek0Anna Sudakova1Jiri Smilek2Duc Anh Nguyen3Hung Le Ngoc4Le Minh Ha5Central European Institute of Technology (CEITEC), Brno University of Technology, Purkynova 123, 612 00 Brno, Czech RepublicCentral European Institute of Technology (CEITEC), Brno University of Technology, Purkynova 123, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 464/118, 612 00 Brno, Czech RepublicCenter for Research and Technology Transfer (CRETECH), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamCenter for Research and Technology Transfer (CRETECH), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamInstitute of Natural Products Chemistry (INPC), Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi 100000, VietnamThis manuscript explores the interaction between methylene blue dye and gelatin within a membrane using spectroscopy and image analysis. Emphasis is placed on methylene blue’s unique properties, specifically its ability to oscillate between two distinct resonance states, each with unique light absorption characteristics. Image analysis serves as a tool for examining dye diffusion and absorption. The results indicate a correlation between dye concentrations and membrane thickness. Thinner layers exhibit a consistent dye concentration, implying an even distribution of the dye during the diffusion process. However, thicker layers display varying concentrations at different edges, suggesting the establishment of a diffusion gradient. Moreover, the authors observe an increased concentration of gelatin at the peripheries rather than at the center, possibly due to the swelling of the dried sample and a potential water concentration gradient. The manuscript concludes by suggesting image analysis as a practical alternative to spectral analysis, particularly for detecting whether methylene blue has been adsorbed onto the macromolecular network. These findings significantly enhance the understanding of the complex interactions between methylene blue and gelatin in a membrane and lay a solid foundation for future research in this field.https://www.mdpi.com/2310-2861/9/11/888methylene bluegelatinimage analysisdye bindingresonance stateslayered materials |
spellingShingle | Jan Zidek Anna Sudakova Jiri Smilek Duc Anh Nguyen Hung Le Ngoc Le Minh Ha Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process Gels methylene blue gelatin image analysis dye binding resonance states layered materials |
title | Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process |
title_full | Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process |
title_fullStr | Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process |
title_full_unstemmed | Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process |
title_short | Explorative Image Analysis of Methylene Blue Interactions with Gelatin in Polypropylene Nonwoven Fabric Membranes: A Potential Future Tool for the Characterization of the Diffusion Process |
title_sort | explorative image analysis of methylene blue interactions with gelatin in polypropylene nonwoven fabric membranes a potential future tool for the characterization of the diffusion process |
topic | methylene blue gelatin image analysis dye binding resonance states layered materials |
url | https://www.mdpi.com/2310-2861/9/11/888 |
work_keys_str_mv | AT janzidek explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess AT annasudakova explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess AT jirismilek explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess AT ducanhnguyen explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess AT hunglengoc explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess AT leminhha explorativeimageanalysisofmethyleneblueinteractionswithgelatininpolypropylenenonwovenfabricmembranesapotentialfuturetoolforthecharacterizationofthediffusionprocess |