Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand
Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | http://www.mdpi.com/2306-7381/5/1/8 |
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| author | Shirli Notcovich Gina DeNicolo Steve H. Flint Norman B. Williamson Kristene Gedye Alex Grinberg Nicolas Lopez-Villalobos |
| author_facet | Shirli Notcovich Gina DeNicolo Steve H. Flint Norman B. Williamson Kristene Gedye Alex Grinberg Nicolas Lopez-Villalobos |
| author_sort | Shirli Notcovich |
| collection | DOAJ |
| description | Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from clinical mastitis cases. Congo red agar (CRA) and microtitre test assay (MTA) in vitro phenotypic tests were used to evaluate biofilm formation. The presence of icaA, icaD, and bap genes associated with biofilm formation was confirmed using the polymerase chain reaction. Results show that most of the S. aureus isolates, though not possessing one of the biofilm-forming genes, were able to produce biofilms. MTA was more frequently positive in identifying biofilm-forming isolates than CRA. |
| first_indexed | 2024-12-20T03:52:40Z |
| format | Article |
| id | doaj.art-6dc1626f8bb249ebae5d56310fa0cf63 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-12-20T03:52:40Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-6dc1626f8bb249ebae5d56310fa0cf632022-12-21T19:54:26ZengMDPI AGVeterinary Sciences2306-73812018-01-0151810.3390/vetsci5010008vetsci5010008Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New ZealandShirli Notcovich0Gina DeNicolo1Steve H. Flint2Norman B. Williamson3Kristene Gedye4Alex Grinberg5Nicolas Lopez-Villalobos6School of Veterinary Science, Massey University, Palmerston North 4410, New ZealandEstendart Limited, Palmerston North 4440, New ZealandMassey Institute of Food Science and Technology, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandBiofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from clinical mastitis cases. Congo red agar (CRA) and microtitre test assay (MTA) in vitro phenotypic tests were used to evaluate biofilm formation. The presence of icaA, icaD, and bap genes associated with biofilm formation was confirmed using the polymerase chain reaction. Results show that most of the S. aureus isolates, though not possessing one of the biofilm-forming genes, were able to produce biofilms. MTA was more frequently positive in identifying biofilm-forming isolates than CRA.http://www.mdpi.com/2306-7381/5/1/8biofilmsbovine mastitisStaphylococcus aureusIcaAIcaDbap |
| spellingShingle | Shirli Notcovich Gina DeNicolo Steve H. Flint Norman B. Williamson Kristene Gedye Alex Grinberg Nicolas Lopez-Villalobos Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand Veterinary Sciences biofilms bovine mastitis Staphylococcus aureus IcaA IcaD bap |
| title | Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand |
| title_full | Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand |
| title_fullStr | Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand |
| title_full_unstemmed | Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand |
| title_short | Biofilm-Forming Potential of Staphylococcus aureus Isolated from Clinical Mastitis Cases in New Zealand |
| title_sort | biofilm forming potential of staphylococcus aureus isolated from clinical mastitis cases in new zealand |
| topic | biofilms bovine mastitis Staphylococcus aureus IcaA IcaD bap |
| url | http://www.mdpi.com/2306-7381/5/1/8 |
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