Succinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain

Succinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain

A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazoliu...

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Bibliographic Details
Main Authors:	Joanna Berlowska, Wojciech Ambroziak, Dorota Kregiel
Format:	Article
Language:	English
Published:	University of Zagreb Faculty of Food Technology and Biotechnology 2008-01-01
Series:	Food Technology and Biotechnology
Subjects:	succinate dehydrogenase (SDH) Saccharomyces cerevisiae enzymatic activity in situ blue tetrazolium
Online Access:	http://hrcak.srce.hr/file/48117

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