Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors
Objective: To evaluate the larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri endocarp against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquitos. Methods: Larvicidal activity was tested according to World Health Organization standard pr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=5;spage=273;epage=278;aulast=Thongwat |
| _version_ | 1818903482117128192 |
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| author | Damrongpan Thongwat Ratchanaporn Chokchaisiri Lucksagoon Ganranoo Nophawan Bunchu |
| author_facet | Damrongpan Thongwat Ratchanaporn Chokchaisiri Lucksagoon Ganranoo Nophawan Bunchu |
| author_sort | Damrongpan Thongwat |
| collection | DOAJ |
| description | Objective: To evaluate the larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri endocarp against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquitos. Methods: Larvicidal activity was tested according to World Health Organization standard protocol. The third-stage larvae of each mosquito species were exposed to various concentrations of Dracaena loureiri crude extract and six groups of Dracaena loureiri fractionated extracts (RC-DT 009–014). Larval mortality rates were observed after 24 h and 48 h of exposure. Then, a computerized probit analysis of the mortality data was performed to determine lethal concentration 50 (LC50) and lethal concentration 90 values. Results: Anopheles minimus larvae (24-h LC50 77.88 mg/L) had the highest susceptibility to crude extract, whereas others (Aedes aegypti, 24-h LC50 224.73 mg/L; Aedes albopictus, 24-h LC50 261.75 mg/L; and Culex quinquefasciatus, 24-h LC50 282.86 mg/L) were significantly less susceptible. The most effective groups of fractionated extracts were RC-DT 012 and RC-DT 013. The mosquito species most susceptible to fractionated extracts was Culex quinquefasciatus, with 24-h LC50 values of 0.66 and 0.94 mg/L for RC-DT 012 and RC-DT 013, respectively. Conclusions: The larvicidal activity of fractionated extracts is more effective than that of crude extract against all tested mosquito species. For the most effective alternative larvicide, purification and a phytochemical constituent analysis must be performed. |
| first_indexed | 2024-12-19T20:52:14Z |
| format | Article |
| id | doaj.art-6dca146b5300497a80593f711c70eefa |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-19T20:52:14Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-6dca146b5300497a80593f711c70eefa2022-12-21T20:06:05ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912018-01-018527327810.4103/2221-1691.233009Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectorsDamrongpan ThongwatRatchanaporn ChokchaisiriLucksagoon GanranooNophawan BunchuObjective: To evaluate the larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri endocarp against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquitos. Methods: Larvicidal activity was tested according to World Health Organization standard protocol. The third-stage larvae of each mosquito species were exposed to various concentrations of Dracaena loureiri crude extract and six groups of Dracaena loureiri fractionated extracts (RC-DT 009–014). Larval mortality rates were observed after 24 h and 48 h of exposure. Then, a computerized probit analysis of the mortality data was performed to determine lethal concentration 50 (LC50) and lethal concentration 90 values. Results: Anopheles minimus larvae (24-h LC50 77.88 mg/L) had the highest susceptibility to crude extract, whereas others (Aedes aegypti, 24-h LC50 224.73 mg/L; Aedes albopictus, 24-h LC50 261.75 mg/L; and Culex quinquefasciatus, 24-h LC50 282.86 mg/L) were significantly less susceptible. The most effective groups of fractionated extracts were RC-DT 012 and RC-DT 013. The mosquito species most susceptible to fractionated extracts was Culex quinquefasciatus, with 24-h LC50 values of 0.66 and 0.94 mg/L for RC-DT 012 and RC-DT 013, respectively. Conclusions: The larvicidal activity of fractionated extracts is more effective than that of crude extract against all tested mosquito species. For the most effective alternative larvicide, purification and a phytochemical constituent analysis must be performed.http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=5;spage=273;epage=278;aulast=Thongwatdracaena loureiriaedes aegyptiaedes albopictusculex quinquefasciatusanopheles minimus fractionated extractcrude extractlarvicidalmosquito larva |
| spellingShingle | Damrongpan Thongwat Ratchanaporn Chokchaisiri Lucksagoon Ganranoo Nophawan Bunchu Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors Asian Pacific Journal of Tropical Biomedicine dracaena loureiri aedes aegypti aedes albopictus culex quinquefasciatus anopheles minimus fractionated extract crude extract larvicidal mosquito larva |
| title | Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors |
| title_full | Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors |
| title_fullStr | Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors |
| title_full_unstemmed | Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors |
| title_short | Larvicidal efficacy of crude and fractionated extracts of Dracaena loureiri Gagnep against Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles minimus mosquito vectors |
| title_sort | larvicidal efficacy of crude and fractionated extracts of dracaena loureiri gagnep against aedes aegypti aedes albopictus culex quinquefasciatus and anopheles minimus mosquito vectors |
| topic | dracaena loureiri aedes aegypti aedes albopictus culex quinquefasciatus anopheles minimus fractionated extract crude extract larvicidal mosquito larva |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=5;spage=273;epage=278;aulast=Thongwat |
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