Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae)
In spite of its incidence on pistachio trees, the chemical ecology of <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) has been neglected so far. In this work, we provide the first evidence of a biologically active male-specific compound that may be promoting field agg...
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MDPI AG
2023-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/2/107 |
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| author | Sergio López Sara Rodrigo-Gómez Enrique Fernández-Carrillo Clàudia Corbella-Martorell Carmen Quero |
| author_facet | Sergio López Sara Rodrigo-Gómez Enrique Fernández-Carrillo Clàudia Corbella-Martorell Carmen Quero |
| author_sort | Sergio López |
| collection | DOAJ |
| description | In spite of its incidence on pistachio trees, the chemical ecology of <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) has been neglected so far. In this work, we provide the first evidence of a biologically active male-specific compound that may be promoting field aggregation. Headspace collections through solid-phase microextraction from feral males and females reported the presence of 2-isobutyl-3-methoxypyrazine exclusively in males. Electroantennographic recordings revealed that males and females responded in a dose-dependent manner to increasing stimuli of 2-isobutyl-3-methoxypyrazine, with females overall displaying a higher response than males. In dual-choice tests, both males and females showed a significant preference for the compound in comparison to a pure air stimulus. In light of these results, the possible role of 2-isobutyl-3-methoxypyrazine as an aggregation cue in <i>L. lusitanica</i> is discussed. |
| first_indexed | 2024-03-11T08:39:42Z |
| format | Article |
| id | doaj.art-6dce7e8529fc46bb8bcdeb6fa22ca4a8 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T08:39:42Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-6dce7e8529fc46bb8bcdeb6fa22ca4a82023-11-16T21:13:56ZengMDPI AGInsects2075-44502023-01-0114210710.3390/insects14020107Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae)Sergio López0Sara Rodrigo-Gómez1Enrique Fernández-Carrillo2Clàudia Corbella-Martorell3Carmen Quero4Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, SpainInstituto Regional de Investigación y Desarrollo Agroalimentario y Forestal (IRIAF)-Centro de Investigación Agroambiental “El Chaparrillo”, 13071 Ciudad Real, SpainInstituto Regional de Investigación y Desarrollo Agroalimentario y Forestal (IRIAF)-Centro de Investigación Agroambiental “El Chaparrillo”, 13071 Ciudad Real, SpainDepartment of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, SpainDepartment of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, SpainIn spite of its incidence on pistachio trees, the chemical ecology of <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) has been neglected so far. In this work, we provide the first evidence of a biologically active male-specific compound that may be promoting field aggregation. Headspace collections through solid-phase microextraction from feral males and females reported the presence of 2-isobutyl-3-methoxypyrazine exclusively in males. Electroantennographic recordings revealed that males and females responded in a dose-dependent manner to increasing stimuli of 2-isobutyl-3-methoxypyrazine, with females overall displaying a higher response than males. In dual-choice tests, both males and females showed a significant preference for the compound in comparison to a pure air stimulus. In light of these results, the possible role of 2-isobutyl-3-methoxypyrazine as an aggregation cue in <i>L. lusitanica</i> is discussed.https://www.mdpi.com/2075-4450/14/2/107<i>Labidostomis lusitanica</i>Chrysomelidae<i>Pistacia vera</i>2-isobutyl-3-methoxypyrazineelectroantennographybehavior |
| spellingShingle | Sergio López Sara Rodrigo-Gómez Enrique Fernández-Carrillo Clàudia Corbella-Martorell Carmen Quero Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) Insects <i>Labidostomis lusitanica</i> Chrysomelidae <i>Pistacia vera</i> 2-isobutyl-3-methoxypyrazine electroantennography behavior |
| title | Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) |
| title_full | Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) |
| title_fullStr | Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) |
| title_full_unstemmed | Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) |
| title_short | Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in <i>Labidostomis lusitanica</i> (Germar) (Coleoptera: Chrysomelidae) |
| title_sort | laboratory evidence of 2 isobutyl 3 methoxypyrazine as a male released aggregative cue in i labidostomis lusitanica i germar coleoptera chrysomelidae |
| topic | <i>Labidostomis lusitanica</i> Chrysomelidae <i>Pistacia vera</i> 2-isobutyl-3-methoxypyrazine electroantennography behavior |
| url | https://www.mdpi.com/2075-4450/14/2/107 |
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