<i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape
Flavan-3-ols are monomers of Proanthocyanidins (PAs), which are important polyphenolic compounds in grapes. Previous studies had shown that <i>VvMYBPA2</i> was closely related to grape flavan-3-ol monomers biosynthesis, but its regulatory network is still unclear. Here, we found that the...
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MDPI AG
2022-09-01
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| author | Changmei Liang Jianyong Guo Mingxiang Chen Xuehui Zhang Guorong Zhang Pengfei Zhang Jinjun Liang Pengfei Wen |
| author_facet | Changmei Liang Jianyong Guo Mingxiang Chen Xuehui Zhang Guorong Zhang Pengfei Zhang Jinjun Liang Pengfei Wen |
| author_sort | Changmei Liang |
| collection | DOAJ |
| description | Flavan-3-ols are monomers of Proanthocyanidins (PAs), which are important polyphenolic compounds in grapes. Previous studies had shown that <i>VvMYBPA2</i> was closely related to grape flavan-3-ol monomers biosynthesis, but its regulatory network is still unclear. Here, we found that the contents of (+)-catechin and (−)-epicatechin, the enzyme activities of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) and the expression of <i>VvANR</i> and <i>VvLAR1</i> were increased in the <i>VvMYBPA2</i> overexpression grape leaves compared to the control. It was proved that VvMYBPA2 protein interacted with VvWDR1 and VvWDR1 protein interacted with VvMYC2 by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). The promoters of <i>VvANR</i> and <i>VvLAR1</i> were bound by VvMYBPA2 using yeast one-hybrid (Y1H) assay. These results suggested that VvMYBPA2 could form a trimeric complex with VvWDR1 and VvMYC2 and jointly regulated the expression of flavan-3-ol monomers related genes <i>VvANR</i> and <i>VvLAR1</i>, thereby affecting the enzyme activities of ANR and LAR and ultimately regulating the contents of flavan-3-ols. |
| first_indexed | 2024-03-10T01:01:33Z |
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| id | doaj.art-6dce87e081464a32aeae7e9481ab6eb2 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T01:01:33Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj.art-6dce87e081464a32aeae7e9481ab6eb22023-11-23T14:33:33ZengMDPI AGAgriculture2077-04722022-09-01129141410.3390/agriculture12091414<i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ GrapeChangmei Liang0Jianyong Guo1Mingxiang Chen2Xuehui Zhang3Guorong Zhang4Pengfei Zhang5Jinjun Liang6Pengfei Wen7College of Information Science and Engineering, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaFlavan-3-ols are monomers of Proanthocyanidins (PAs), which are important polyphenolic compounds in grapes. Previous studies had shown that <i>VvMYBPA2</i> was closely related to grape flavan-3-ol monomers biosynthesis, but its regulatory network is still unclear. Here, we found that the contents of (+)-catechin and (−)-epicatechin, the enzyme activities of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) and the expression of <i>VvANR</i> and <i>VvLAR1</i> were increased in the <i>VvMYBPA2</i> overexpression grape leaves compared to the control. It was proved that VvMYBPA2 protein interacted with VvWDR1 and VvWDR1 protein interacted with VvMYC2 by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). The promoters of <i>VvANR</i> and <i>VvLAR1</i> were bound by VvMYBPA2 using yeast one-hybrid (Y1H) assay. These results suggested that VvMYBPA2 could form a trimeric complex with VvWDR1 and VvMYC2 and jointly regulated the expression of flavan-3-ol monomers related genes <i>VvANR</i> and <i>VvLAR1</i>, thereby affecting the enzyme activities of ANR and LAR and ultimately regulating the contents of flavan-3-ols.https://www.mdpi.com/2077-0472/12/9/1414grapeflavan-3-olsproanthocyanidins<i>VvMYBPA2</i>MBW complex |
| spellingShingle | Changmei Liang Jianyong Guo Mingxiang Chen Xuehui Zhang Guorong Zhang Pengfei Zhang Jinjun Liang Pengfei Wen <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape Agriculture grape flavan-3-ols proanthocyanidins <i>VvMYBPA2</i> MBW complex |
| title | <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape |
| title_full | <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape |
| title_fullStr | <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape |
| title_full_unstemmed | <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape |
| title_short | <i>VvMYBPA2</i> Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape |
| title_sort | i vvmybpa2 i regulated the accumulation of flavan 3 ols though forming a trimeric complex in zaoheibao grape |
| topic | grape flavan-3-ols proanthocyanidins <i>VvMYBPA2</i> MBW complex |
| url | https://www.mdpi.com/2077-0472/12/9/1414 |
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