A Simple Method for Sexing Chicken Embryos at Stage X
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the ex...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2003-07-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/40/3/40_3_226/_pdf/-char/en |
| _version_ | 1797816943201222656 |
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| author | Mitsuru Naito Akiko Sano Takaharu Kawashima Hiroyuki Nakamichi Takashi Harumi Yuko Matsubara Takashi Kuwana |
| author_facet | Mitsuru Naito Akiko Sano Takaharu Kawashima Hiroyuki Nakamichi Takashi Harumi Yuko Matsubara Takashi Kuwana |
| author_sort | Mitsuru Naito |
| collection | DOAJ |
| description | A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the extraction method (DNA extraction kit) as a control. PCR analysis was carried out using the prepared DNA, and the sex of embryos was clearly identified by both the boiling and extraction method. This simple and rapid method of embryo sexing using blastodermal cells isolated from the area opaca enables us to identify the sex of a large number of chicken embryos in a limited time. |
| first_indexed | 2024-03-13T08:45:09Z |
| format | Article |
| id | doaj.art-6dd003f7b6a240be9ef44552e1705b3e |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:09Z |
| publishDate | 2003-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-6dd003f7b6a240be9ef44552e1705b3e2023-05-30T05:57:14ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862003-07-0140322623010.2141/jpsa.40.226jpsaA Simple Method for Sexing Chicken Embryos at Stage XMitsuru Naito0Akiko Sano1Takaharu Kawashima2Hiroyuki Nakamichi3Takashi Harumi4Yuko Matsubara5Takashi Kuwana6National Institute of Agrobiological SciencesNational Institute of Agrobiological SciencesNational Institute of Agrobiological SciencesNational Institute of Agrobiological SciencesNational Institute of Agrobiological SciencesNational Institute of Agrobiological SciencesNational Institute for Minamata DiseaseA simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute centrifugation). DNA was also prepared by the extraction method (DNA extraction kit) as a control. PCR analysis was carried out using the prepared DNA, and the sex of embryos was clearly identified by both the boiling and extraction method. This simple and rapid method of embryo sexing using blastodermal cells isolated from the area opaca enables us to identify the sex of a large number of chicken embryos in a limited time.https://www.jstage.jst.go.jp/article/jpsa/40/3/40_3_226/_pdf/-char/enblastodermal cellchicken embryoembryo culturepcrsexing |
| spellingShingle | Mitsuru Naito Akiko Sano Takaharu Kawashima Hiroyuki Nakamichi Takashi Harumi Yuko Matsubara Takashi Kuwana A Simple Method for Sexing Chicken Embryos at Stage X The Journal of Poultry Science blastodermal cell chicken embryo embryo culture pcr sexing |
| title | A Simple Method for Sexing Chicken Embryos at Stage X |
| title_full | A Simple Method for Sexing Chicken Embryos at Stage X |
| title_fullStr | A Simple Method for Sexing Chicken Embryos at Stage X |
| title_full_unstemmed | A Simple Method for Sexing Chicken Embryos at Stage X |
| title_short | A Simple Method for Sexing Chicken Embryos at Stage X |
| title_sort | simple method for sexing chicken embryos at stage x |
| topic | blastodermal cell chicken embryo embryo culture pcr sexing |
| url | https://www.jstage.jst.go.jp/article/jpsa/40/3/40_3_226/_pdf/-char/en |
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