Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing
<p>Abstract</p> <p>Background</p> <p>Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D <it>in vivo </it>tissue growth. Such realistic environmen...
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| Format: | Article |
| Language: | English |
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BMC
2007-12-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/7/88 |
| _version_ | 1819086212380491776 |
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| author | Akpe Victor Rydholm Susanna Liebmann Thomas Brismar Hjalmar |
| author_facet | Akpe Victor Rydholm Susanna Liebmann Thomas Brismar Hjalmar |
| author_sort | Akpe Victor |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D <it>in vivo </it>tissue growth. Such realistic environments should be the aim of any cell growth study, requiring new methods for culturing cells <it>in vitro</it>. Cell biology is also tending toward miniaturization for increased efficiency and specificity. This paper discusses the application of a self-assembling peptide-derived hydrogel for use as a 3D cell culture scaffold at the microscale.</p> <p>Results</p> <p>Phenylalanine derivative hydrogel formation was seen to occur in multiple dispersion media. Cells were immobilized <it>in situ </it>within microchambers designed for cell analysis. Use of the highly biocompatible hydrogel components and simplistic procedures significantly reduced the cytotoxic effects seen with alternate 3D culture materials and microstructure loading methods. Cells were easily immobilized, sustained and removed from microchambers. Differences in growth morphology were seen in the cultured cells, owing to the 3-dimentional character of the gel structure. Degradation improved the removal of hydrogel from the microstructures, permitting reuse of the analysis platforms.</p> <p>Conclusion</p> <p>Self-assembling diphenylalanine derivative hydrogel provided a method to dramatically reduce the typical difficulties of microculture formation. Effective generation of patterned 3D cultures will lead to improved cell study results by better modeling <it>in vivo </it>growth environments and increasing efficiency and specificity of cell studies. Use of simplified growth scaffolds such as peptide-derived hydrogel should be seen as highly advantageous and will likely become more commonplace in cell culture methodology.</p> |
| first_indexed | 2024-12-21T21:16:40Z |
| format | Article |
| id | doaj.art-6dd05642adb449fb8c18d57d4440fb12 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-21T21:16:40Z |
| publishDate | 2007-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-6dd05642adb449fb8c18d57d4440fb122022-12-21T18:49:59ZengBMCBMC Biotechnology1472-67502007-12-01718810.1186/1472-6750-7-88Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturingAkpe VictorRydholm SusannaLiebmann ThomasBrismar Hjalmar<p>Abstract</p> <p>Background</p> <p>Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D <it>in vivo </it>tissue growth. Such realistic environments should be the aim of any cell growth study, requiring new methods for culturing cells <it>in vitro</it>. Cell biology is also tending toward miniaturization for increased efficiency and specificity. This paper discusses the application of a self-assembling peptide-derived hydrogel for use as a 3D cell culture scaffold at the microscale.</p> <p>Results</p> <p>Phenylalanine derivative hydrogel formation was seen to occur in multiple dispersion media. Cells were immobilized <it>in situ </it>within microchambers designed for cell analysis. Use of the highly biocompatible hydrogel components and simplistic procedures significantly reduced the cytotoxic effects seen with alternate 3D culture materials and microstructure loading methods. Cells were easily immobilized, sustained and removed from microchambers. Differences in growth morphology were seen in the cultured cells, owing to the 3-dimentional character of the gel structure. Degradation improved the removal of hydrogel from the microstructures, permitting reuse of the analysis platforms.</p> <p>Conclusion</p> <p>Self-assembling diphenylalanine derivative hydrogel provided a method to dramatically reduce the typical difficulties of microculture formation. Effective generation of patterned 3D cultures will lead to improved cell study results by better modeling <it>in vivo </it>growth environments and increasing efficiency and specificity of cell studies. Use of simplified growth scaffolds such as peptide-derived hydrogel should be seen as highly advantageous and will likely become more commonplace in cell culture methodology.</p>http://www.biomedcentral.com/1472-6750/7/88 |
| spellingShingle | Akpe Victor Rydholm Susanna Liebmann Thomas Brismar Hjalmar Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing BMC Biotechnology |
| title | Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing |
| title_full | Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing |
| title_fullStr | Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing |
| title_full_unstemmed | Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing |
| title_short | Self-assembling Fmoc dipeptide hydrogel for <it>in situ </it>3D cell culturing |
| title_sort | self assembling fmoc dipeptide hydrogel for it in situ it 3d cell culturing |
| url | http://www.biomedcentral.com/1472-6750/7/88 |
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