Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton
Entomopathogenic fungi produces endochitianses, involved in the degradation of insect chitin to facilitate the infection process. Endochitinases (Chit1) gene of family 18 glycosyl hydrolyses were amplified, cloned and characterized from genomic DNA of two isolates of Metarhizium anisopliae. Catalyti...
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| Format: | Article |
| Language: | English |
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The Royal Society
2019-08-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190412 |
| _version_ | 1819092185464700928 |
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| author | Waheed Anwar Muhammad Asim Javed Ahmad Ali Shahid Kiran Nawaz Adnan Akhter Muhammad Zia Ur Rehman Usman Hameed Sehrish Iftikhar Muhammad Saleem Haider |
| author_facet | Waheed Anwar Muhammad Asim Javed Ahmad Ali Shahid Kiran Nawaz Adnan Akhter Muhammad Zia Ur Rehman Usman Hameed Sehrish Iftikhar Muhammad Saleem Haider |
| author_sort | Waheed Anwar |
| collection | DOAJ |
| description | Entomopathogenic fungi produces endochitianses, involved in the degradation of insect chitin to facilitate the infection process. Endochitinases (Chit1) gene of family 18 glycosyl hydrolyses were amplified, cloned and characterized from genomic DNA of two isolates of Metarhizium anisopliae. Catalytic motif of family 18 glycosyl hydrolyses was found in Chit1 of M. anisopliae, while no signal peptide was found in any isolate, whereas substrate-binding motif was found in Chit1 of both isolates. Phylogenetic analysis revealed the evolutionary relationship among the fungal chitinases of Metarhizium. The Chit1 amplified were closely related to the family 18 glycosyl hydrolyses. Transient expressions of Chit1 in cotton plants using Geminivirus-mediated gene silencing vector of Cotton Leaf Crumple Virus (CLCrV) revealed the chitinase activity of Chit1 genes amplified from both of the isolates of M. anisopliae when compared with the control. Transformed cotton plants were virulent against fourth instar nymphal and adult stages of Bemisia tabaci which resulted in the mortality of both fourth instar nymphal and adult B. tabaci. Thus, the fungal chitinases expressed in cotton plants played a vital role in plant defence against B. tabaci. However, further studies are required to explore the comparative effectiveness of chitinases from different fungal strains against economically important insect pests. |
| first_indexed | 2024-12-21T22:51:36Z |
| format | Article |
| id | doaj.art-6dd3e00eaa3c407aa7573f1075431c69 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-21T22:51:36Z |
| publishDate | 2019-08-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-6dd3e00eaa3c407aa7573f1075431c692022-12-21T18:47:34ZengThe Royal SocietyRoyal Society Open Science2054-57032019-08-016810.1098/rsos.190412190412Chitinase genes from Metarhizium anisopliae for the control of whitefly in cottonWaheed AnwarMuhammad Asim JavedAhmad Ali ShahidKiran NawazAdnan AkhterMuhammad Zia Ur RehmanUsman HameedSehrish IftikharMuhammad Saleem HaiderEntomopathogenic fungi produces endochitianses, involved in the degradation of insect chitin to facilitate the infection process. Endochitinases (Chit1) gene of family 18 glycosyl hydrolyses were amplified, cloned and characterized from genomic DNA of two isolates of Metarhizium anisopliae. Catalytic motif of family 18 glycosyl hydrolyses was found in Chit1 of M. anisopliae, while no signal peptide was found in any isolate, whereas substrate-binding motif was found in Chit1 of both isolates. Phylogenetic analysis revealed the evolutionary relationship among the fungal chitinases of Metarhizium. The Chit1 amplified were closely related to the family 18 glycosyl hydrolyses. Transient expressions of Chit1 in cotton plants using Geminivirus-mediated gene silencing vector of Cotton Leaf Crumple Virus (CLCrV) revealed the chitinase activity of Chit1 genes amplified from both of the isolates of M. anisopliae when compared with the control. Transformed cotton plants were virulent against fourth instar nymphal and adult stages of Bemisia tabaci which resulted in the mortality of both fourth instar nymphal and adult B. tabaci. Thus, the fungal chitinases expressed in cotton plants played a vital role in plant defence against B. tabaci. However, further studies are required to explore the comparative effectiveness of chitinases from different fungal strains against economically important insect pests.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190412endochitinaseentomopathogenic fungibemisia tabacichitinase activityinsect resistancevirus-induced gene silencing |
| spellingShingle | Waheed Anwar Muhammad Asim Javed Ahmad Ali Shahid Kiran Nawaz Adnan Akhter Muhammad Zia Ur Rehman Usman Hameed Sehrish Iftikhar Muhammad Saleem Haider Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton Royal Society Open Science endochitinase entomopathogenic fungi bemisia tabaci chitinase activity insect resistance virus-induced gene silencing |
| title | Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton |
| title_full | Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton |
| title_fullStr | Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton |
| title_full_unstemmed | Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton |
| title_short | Chitinase genes from Metarhizium anisopliae for the control of whitefly in cotton |
| title_sort | chitinase genes from metarhizium anisopliae for the control of whitefly in cotton |
| topic | endochitinase entomopathogenic fungi bemisia tabaci chitinase activity insect resistance virus-induced gene silencing |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190412 |
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