Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene
Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family,...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-06-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201602-0005_enhanced-tolerance-to-low-k-stress-in-tobacco-plants-that-ectopically-express-the-cbl-interacting-protein-kin.php |
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| author | Gang XUE Li-Ming LU Tie-Zhao YANG Xiao-Hui LI Xue-Xia XING Shi-Xiao XU |
| author_facet | Gang XUE Li-Ming LU Tie-Zhao YANG Xiao-Hui LI Xue-Xia XING Shi-Xiao XU |
| author_sort | Gang XUE |
| collection | DOAJ |
| description | Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress. |
| first_indexed | 2024-04-10T08:29:26Z |
| format | Article |
| id | doaj.art-6dd649d771fc4e28a4c696fdd2e77423 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:26Z |
| publishDate | 2016-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-6dd649d771fc4e28a4c696fdd2e774232023-02-23T03:30:33ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252016-06-01522778210.17221/155/2015-CJGPBcjg-201602-0005Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 geneGang XUE0Li-Ming LU1Tie-Zhao YANG2Xiao-Hui LI3Xue-Xia XING4Shi-Xiao XU5College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. ChinaCollege of Agronomy, Sichuan Agricultural University, Ya'an, P.R. ChinaCollege of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. ChinaCollege of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. ChinaCollege of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. ChinaCollege of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. ChinaTobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress.https://cjgpb.agriculturejournals.cz/artkey/cjg-201602-0005_enhanced-tolerance-to-low-k-stress-in-tobacco-plants-that-ectopically-express-the-cbl-interacting-protein-kin.phpatcipk23k+ uptakelow-k+ tolerancepotassiumtobacco |
| spellingShingle | Gang XUE Li-Ming LU Tie-Zhao YANG Xiao-Hui LI Xue-Xia XING Shi-Xiao XU Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene Czech Journal of Genetics and Plant Breeding atcipk23 k+ uptake low-k+ tolerance potassium tobacco |
| title | Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene |
| title_full | Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene |
| title_fullStr | Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene |
| title_full_unstemmed | Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene |
| title_short | Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene |
| title_sort | enhanced tolerance to low k stress in tobacco plants that ectopically express the cbl interacting protein kinase cipk23 gene |
| topic | atcipk23 k+ uptake low-k+ tolerance potassium tobacco |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201602-0005_enhanced-tolerance-to-low-k-stress-in-tobacco-plants-that-ectopically-express-the-cbl-interacting-protein-kin.php |
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