Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia
Background and objective: Protein phosphatase 3 catalytic subunit gamma (PPP3CC) and protein phosphatase 3 regulatory subunit B, beta (PPP3R2) are respectively the catalytic and regulatory subunits of calcineurin in sperm. Deficiency in either protein causes impaired sperm motility leading to mal...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MRE Press
2021-09-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/jomh/article/20210928-462/pdf/JOMH2021021801.pdf |
| _version_ | 1827359730378473472 |
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| author | Hang Kang Chen Chen Feng-Xin Gao Xu-Hui Zeng Xiao-Ning Zhang |
| author_facet | Hang Kang Chen Chen Feng-Xin Gao Xu-Hui Zeng Xiao-Ning Zhang |
| author_sort | Hang Kang |
| collection | DOAJ |
| description | Background and objective: Protein phosphatase 3 catalytic subunit gamma
(PPP3CC) and protein phosphatase 3 regulatory subunit B, beta (PPP3R2) are
respectively the catalytic and regulatory subunits of calcineurin in sperm.
Deficiency in either protein causes impaired sperm motility leading to male
infertility. Many cases of sterility are
attributed to asthenozoospermia (AZS); however,
it remains unknown whether PPP3CC and PPP3R2
are related to AZS.
Material and methods: Quantitative PCR and Western blotting were used
to investigate the expression levels of PPP3CC and PPP3R2 in the spermatozoa of
patients with AZS and to explore the clinical significance.
Results: Two calcineurin inhibitors cyclosporine A (CsA) and tacrolimus
(FK506) markedly impaired the total motility
and progressive motility of human sperm, indicating that PPP3CC or PPP3R2 might
be involved in AZS. PPP3CC mRNA and protein expression was lower in the
ejaculated spermatozoa of patients with AZS than in normal sperm (NS).
Correlation analysis showed that PPP3CC protein expression correlated positively
with progressive motility (r = 0.2592, P < 0.05); however, there were
no significant differences in PPP3R2 mRNA and protein levels between AZS and NS.
Conclusion: These findings suggest that the abnormal expression of
PPP3CC rather than PPP3R2 might be a pathological factor or indicator in AZS.
Thus, PPP3CC may be a potential therapeutic or diagnostic target for some cases
of male infertility. |
| first_indexed | 2024-03-08T06:34:20Z |
| format | Article |
| id | doaj.art-6dd6d88c95fa4a4393166274816cf48f |
| institution | Directory Open Access Journal |
| issn | 1875-6859 |
| language | English |
| last_indexed | 2024-03-08T06:34:20Z |
| publishDate | 2021-09-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj.art-6dd6d88c95fa4a4393166274816cf48f2024-02-03T10:53:32ZengMRE PressJournal of Men's Health1875-68592021-09-0117418318910.31083/jomh.2021.039S1875-6867(21)00339-0Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermiaHang Kang0Chen Chen1Feng-Xin Gao2Xu-Hui Zeng3Xiao-Ning Zhang4Institute of Reproductive Medicine, Medical School, Nantong University, 226001 Nantong, ChinaInstitute of Reproductive Medicine, Medical School, Nantong University, 226001 Nantong, ChinaInstitute of Life Science, Nanchang University, 330031 Nanchang, ChinaInstitute of Reproductive Medicine, Medical School, Nantong University, 226001 Nantong, ChinaInstitute of Reproductive Medicine, Medical School, Nantong University, 226001 Nantong, ChinaBackground and objective: Protein phosphatase 3 catalytic subunit gamma (PPP3CC) and protein phosphatase 3 regulatory subunit B, beta (PPP3R2) are respectively the catalytic and regulatory subunits of calcineurin in sperm. Deficiency in either protein causes impaired sperm motility leading to male infertility. Many cases of sterility are attributed to asthenozoospermia (AZS); however, it remains unknown whether PPP3CC and PPP3R2 are related to AZS. Material and methods: Quantitative PCR and Western blotting were used to investigate the expression levels of PPP3CC and PPP3R2 in the spermatozoa of patients with AZS and to explore the clinical significance. Results: Two calcineurin inhibitors cyclosporine A (CsA) and tacrolimus (FK506) markedly impaired the total motility and progressive motility of human sperm, indicating that PPP3CC or PPP3R2 might be involved in AZS. PPP3CC mRNA and protein expression was lower in the ejaculated spermatozoa of patients with AZS than in normal sperm (NS). Correlation analysis showed that PPP3CC protein expression correlated positively with progressive motility (r = 0.2592, P < 0.05); however, there were no significant differences in PPP3R2 mRNA and protein levels between AZS and NS. Conclusion: These findings suggest that the abnormal expression of PPP3CC rather than PPP3R2 might be a pathological factor or indicator in AZS. Thus, PPP3CC may be a potential therapeutic or diagnostic target for some cases of male infertility.https://oss.jomh.org/jomh/article/20210928-462/pdf/JOMH2021021801.pdfasthenozoospermiacalcineurinppp3ccppp3r2sperm motilityprogressive motility |
| spellingShingle | Hang Kang Chen Chen Feng-Xin Gao Xu-Hui Zeng Xiao-Ning Zhang Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia Journal of Men's Health asthenozoospermia calcineurin ppp3cc ppp3r2 sperm motility progressive motility |
| title | Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia |
| title_full | Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia |
| title_fullStr | Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia |
| title_full_unstemmed | Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia |
| title_short | Expression of PPP3CC and not PPP3R2 is associated with asthenozoospermia |
| title_sort | expression of ppp3cc and not ppp3r2 is associated with asthenozoospermia |
| topic | asthenozoospermia calcineurin ppp3cc ppp3r2 sperm motility progressive motility |
| url | https://oss.jomh.org/jomh/article/20210928-462/pdf/JOMH2021021801.pdf |
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