A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens
In Arabidopsis, NPR1 is a key transcriptional coregulator of systemic acquired resistance. Upon pathogen challenge, NPR1 translocates from the cytoplasm to the nucleus, in which it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related (PR) genes. In...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2016-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-16-0207-R |
| _version_ | 1811278113798094848 |
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| author | Xiaodong Wang Baoju Yang Kun Li Zhensheng Kang Dario Cantu Jorge Dubcovsky |
| author_facet | Xiaodong Wang Baoju Yang Kun Li Zhensheng Kang Dario Cantu Jorge Dubcovsky |
| author_sort | Xiaodong Wang |
| collection | DOAJ |
| description | In Arabidopsis, NPR1 is a key transcriptional coregulator of systemic acquired resistance. Upon pathogen challenge, NPR1 translocates from the cytoplasm to the nucleus, in which it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related (PR) genes. In a screen of a yeast two-hybrid library from wheat leaves infected with Puccinia striiformis f. sp. tritici, we identified a conserved rust protein that interacts with wheat NPR1 and named it PNPi (for Puccinia NPR1 interactor). PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal DPBB_1 domain. Using bimolecular fluorescence complementation assays, we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts. A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2. In barley transgenic lines overexpressing PNPi, we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringae pv. tomato DC3000 infection. Based on these results, we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1. |
| first_indexed | 2024-04-13T00:28:50Z |
| format | Article |
| id | doaj.art-6dd7f80e73dc41bf9c1328a70197886d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:28:50Z |
| publishDate | 2016-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6dd7f80e73dc41bf9c1328a70197886d2022-12-22T03:10:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062016-12-01291297798910.1094/MPMI-10-16-0207-RA Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to PathogensXiaodong Wang0Baoju Yang1Kun Li2Zhensheng Kang3Dario Cantu4Jorge Dubcovsky5Department of Plant Science, University of California, Davis, CA 95616, U.S.A.;Department of Plant Science, University of California, Davis, CA 95616, U.S.A.;Department of Plant Science, University of California, Davis, CA 95616, U.S.A.;State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, P. R. China;Department of Viticulture and Enology, University of California, Davis, CA 95616, U.S.A.;Department of Plant Science, University of California, Davis, CA 95616, U.S.A.;In Arabidopsis, NPR1 is a key transcriptional coregulator of systemic acquired resistance. Upon pathogen challenge, NPR1 translocates from the cytoplasm to the nucleus, in which it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related (PR) genes. In a screen of a yeast two-hybrid library from wheat leaves infected with Puccinia striiformis f. sp. tritici, we identified a conserved rust protein that interacts with wheat NPR1 and named it PNPi (for Puccinia NPR1 interactor). PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal DPBB_1 domain. Using bimolecular fluorescence complementation assays, we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts. A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2. In barley transgenic lines overexpressing PNPi, we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringae pv. tomato DC3000 infection. Based on these results, we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-16-0207-R |
| spellingShingle | Xiaodong Wang Baoju Yang Kun Li Zhensheng Kang Dario Cantu Jorge Dubcovsky A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens Molecular Plant-Microbe Interactions |
| title | A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens |
| title_full | A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens |
| title_fullStr | A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens |
| title_full_unstemmed | A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens |
| title_short | A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens |
| title_sort | conserved puccinia striiformis protein interacts with wheat npr1 and reduces induction of pathogenesis related genes in response to pathogens |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-16-0207-R |
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