Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i>
<i>Geotrichum candidum</i> is an environmental yeast, also found as part of the cheese surface microbiota, where it is important in the ripening of many traditional cheeses, such as Camembert. We have previously developed a Multi Locus Sequence Typing (MLST) scheme, which differentiated...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/103 |
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| author | Colin R. Tinsley Noémie Jacques Marine Lucas Cécile Grondin Jean-Luc Legras Serge Casaregola |
| author_facet | Colin R. Tinsley Noémie Jacques Marine Lucas Cécile Grondin Jean-Luc Legras Serge Casaregola |
| author_sort | Colin R. Tinsley |
| collection | DOAJ |
| description | <i>Geotrichum candidum</i> is an environmental yeast, also found as part of the cheese surface microbiota, where it is important in the ripening of many traditional cheeses, such as Camembert. We have previously developed a Multi Locus Sequence Typing (MLST) scheme, which differentiated five clades, of which one contained only environmental isolates, two were composed almost entirely of dairy isolates, and two others contained a mixture of dairy, environmental, and miscellaneous food isolates. In order to provide a simple method to uniquely type <i>G. candidum</i> strains, and in addition to permit investigation of the population structure at a fine level, we describe here a molecular analysis using a set of twelve highly discriminating microsatellite-like markers. The present study consolidates the previously suggested division between dairy and environmental strains, and in addition distinguishes a specifically European group of environmental strains. This analysis permitted the discrimination of 72 genotypes from the collection of 80 isolates, while retaining the underlying meaningful phylogenetic relation between groups of strains. |
| first_indexed | 2024-03-10T00:53:50Z |
| format | Article |
| id | doaj.art-6ddb2dc59b004583ba90613297df93e8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T00:53:50Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6ddb2dc59b004583ba90613297df93e82023-11-23T14:47:24ZengMDPI AGMicroorganisms2076-26072022-01-0110110310.3390/microorganisms10010103Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i>Colin R. Tinsley0Noémie Jacques1Marine Lucas2Cécile Grondin3Jean-Luc Legras4Serge Casaregola5Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceSPO, Université de Montpellier, INRAE, Institut Agro, 34000 Montpellier, FranceMicalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France<i>Geotrichum candidum</i> is an environmental yeast, also found as part of the cheese surface microbiota, where it is important in the ripening of many traditional cheeses, such as Camembert. We have previously developed a Multi Locus Sequence Typing (MLST) scheme, which differentiated five clades, of which one contained only environmental isolates, two were composed almost entirely of dairy isolates, and two others contained a mixture of dairy, environmental, and miscellaneous food isolates. In order to provide a simple method to uniquely type <i>G. candidum</i> strains, and in addition to permit investigation of the population structure at a fine level, we describe here a molecular analysis using a set of twelve highly discriminating microsatellite-like markers. The present study consolidates the previously suggested division between dairy and environmental strains, and in addition distinguishes a specifically European group of environmental strains. This analysis permitted the discrimination of 72 genotypes from the collection of 80 isolates, while retaining the underlying meaningful phylogenetic relation between groups of strains.https://www.mdpi.com/2076-2607/10/1/103<i>Geotrichum</i> <i>candidum</i>adaptationenvironmentdairy |
| spellingShingle | Colin R. Tinsley Noémie Jacques Marine Lucas Cécile Grondin Jean-Luc Legras Serge Casaregola Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> Microorganisms <i>Geotrichum</i> <i>candidum</i> adaptation environment dairy |
| title | Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> |
| title_full | Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> |
| title_fullStr | Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> |
| title_full_unstemmed | Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> |
| title_short | Molecular Genetic Analysis with Microsatellite-like Loci Reveals Specific Dairy-Associated and Environmental Populations of the Yeast <i>Geotrichum candidum</i> |
| title_sort | molecular genetic analysis with microsatellite like loci reveals specific dairy associated and environmental populations of the yeast i geotrichum candidum i |
| topic | <i>Geotrichum</i> <i>candidum</i> adaptation environment dairy |
| url | https://www.mdpi.com/2076-2607/10/1/103 |
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