Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i>
Biofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Drugs used for biofilm-associated infections are limited because biofilm-embedded or biofilm-matrix bacteria are difficult to kill or eradiate. Therefore, many resea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/349 |
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| author | Young-Min Kim Hyosuk Son Seong-Cheol Park Jong-Kook Lee Mi-Kyeong Jang Jung Ro Lee |
| author_facet | Young-Min Kim Hyosuk Son Seong-Cheol Park Jong-Kook Lee Mi-Kyeong Jang Jung Ro Lee |
| author_sort | Young-Min Kim |
| collection | DOAJ |
| description | Biofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Drugs used for biofilm-associated infections are limited because biofilm-embedded or biofilm-matrix bacteria are difficult to kill or eradiate. Therefore, many researchers are developing new and effective antibiofilm agents. Among them, antimicrobial peptides have an attractive interest in the development of antibiofilm agents. The present study evaluated the effects of 10 synthetic peptides on growth inhibition, inhibition of biofilm formation, and biofilm elimination in drug-resistant <i>Pseudomonas aeruginosa</i>. The planktonic cell growth and biofilm formation were dose-dependently inhibited by most of the peptides. WIK-14 eliminated preformed biofilm masses by removing carbohydrates, extracellular nucleic acids, proteins, and lipids constituting extracellular polymeric substances. The results demonstrated that WIK-14 and WIKE-14 peptides might provide novel therapeutic drugs to overcome multidrug resistance in biofilm-associated infections. |
| first_indexed | 2024-03-11T09:15:04Z |
| format | Article |
| id | doaj.art-6ddb95c6edb340168c9623336cc09f12 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:15:04Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-6ddb95c6edb340168c9623336cc09f122023-11-16T18:43:46ZengMDPI AGAntibiotics2079-63822023-02-0112234910.3390/antibiotics12020349Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i>Young-Min Kim0Hyosuk Son1Seong-Cheol Park2Jong-Kook Lee3Mi-Kyeong Jang4Jung Ro Lee5Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaLMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of KoreaBiofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Drugs used for biofilm-associated infections are limited because biofilm-embedded or biofilm-matrix bacteria are difficult to kill or eradiate. Therefore, many researchers are developing new and effective antibiofilm agents. Among them, antimicrobial peptides have an attractive interest in the development of antibiofilm agents. The present study evaluated the effects of 10 synthetic peptides on growth inhibition, inhibition of biofilm formation, and biofilm elimination in drug-resistant <i>Pseudomonas aeruginosa</i>. The planktonic cell growth and biofilm formation were dose-dependently inhibited by most of the peptides. WIK-14 eliminated preformed biofilm masses by removing carbohydrates, extracellular nucleic acids, proteins, and lipids constituting extracellular polymeric substances. The results demonstrated that WIK-14 and WIKE-14 peptides might provide novel therapeutic drugs to overcome multidrug resistance in biofilm-associated infections.https://www.mdpi.com/2079-6382/12/2/349<i>Pseudomonas aeruginosa</i>planktonic cellsantimicrobial peptideantimicrobial resistancebiofilm |
| spellingShingle | Young-Min Kim Hyosuk Son Seong-Cheol Park Jong-Kook Lee Mi-Kyeong Jang Jung Ro Lee Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> Antibiotics <i>Pseudomonas aeruginosa</i> planktonic cells antimicrobial peptide antimicrobial resistance biofilm |
| title | Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> |
| title_full | Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> |
| title_short | Anti-Biofilm Effects of Rationally Designed Peptides against Planktonic Cells and Pre-Formed Biofilm of <i>Pseudomonas aeruginosa</i> |
| title_sort | anti biofilm effects of rationally designed peptides against planktonic cells and pre formed biofilm of i pseudomonas aeruginosa i |
| topic | <i>Pseudomonas aeruginosa</i> planktonic cells antimicrobial peptide antimicrobial resistance biofilm |
| url | https://www.mdpi.com/2079-6382/12/2/349 |
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