Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery.
Inflammatory outcomes, including toxic anterior segment syndrome (TASS) and infectious endophthalmitis, are potentially painful, blinding complications following cataract surgery. In an in vitro pilot study, commercially available, sterile foldable intraocular lenses (IOLs) used during routine canin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0277753 |
| _version_ | 1797959152978362368 |
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| author | Kourtney K Dowler Aida Vientós-Plotts Elizabeth A Giuliano Zachary L McAdams Carol R Reinero Aaron C Ericsson |
| author_facet | Kourtney K Dowler Aida Vientós-Plotts Elizabeth A Giuliano Zachary L McAdams Carol R Reinero Aaron C Ericsson |
| author_sort | Kourtney K Dowler |
| collection | DOAJ |
| description | Inflammatory outcomes, including toxic anterior segment syndrome (TASS) and infectious endophthalmitis, are potentially painful, blinding complications following cataract surgery. In an in vitro pilot study, commercially available, sterile foldable intraocular lenses (IOLs) used during routine canine cataract surgery, and their packaging fluid were surveyed for the presence of bacterial DNA and/or viable (cultivable) bacteria. Swabs from IOLs and packaging fluid from three different veterinary manufacturers and three different production lots/manufacturer were collected for 16S ribosomal ribonucleic acid (rRNA) sequencing. Packaging fluid samples were collected for aerobic/capnophilic bacterial culture. Culture yielded one isolate, identified as Staphylococcus epidermidis. 16S rRNA sequencing revealed distinct brand-specific bacterial DNA profiles, conserved between IOLs and packaging fluid of all production lots within each manufacturer. The dominant taxonomy differentiating each manufacturer was annotated as Staphylococcus sp, and was a 100% match to S. epidermidis. Distinct mixtures of bacterial DNA are present and consistent in IOLs and packaging fluid depending on the manufacturer, and Staphylococcus is the dominant contributor to the bacterial DNA detected. Caralens products had a significantly lower amount of Staphylococcus spp. compared to Anvision and Dioptrix products. |
| first_indexed | 2024-04-11T00:28:25Z |
| format | Article |
| id | doaj.art-6ddc4379ff614f5ab7390a22d314d1ae |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T00:28:25Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6ddc4379ff614f5ab7390a22d314d1ae2023-01-08T05:31:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011711e027775310.1371/journal.pone.0277753Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery.Kourtney K DowlerAida Vientós-PlottsElizabeth A GiulianoZachary L McAdamsCarol R ReineroAaron C EricssonInflammatory outcomes, including toxic anterior segment syndrome (TASS) and infectious endophthalmitis, are potentially painful, blinding complications following cataract surgery. In an in vitro pilot study, commercially available, sterile foldable intraocular lenses (IOLs) used during routine canine cataract surgery, and their packaging fluid were surveyed for the presence of bacterial DNA and/or viable (cultivable) bacteria. Swabs from IOLs and packaging fluid from three different veterinary manufacturers and three different production lots/manufacturer were collected for 16S ribosomal ribonucleic acid (rRNA) sequencing. Packaging fluid samples were collected for aerobic/capnophilic bacterial culture. Culture yielded one isolate, identified as Staphylococcus epidermidis. 16S rRNA sequencing revealed distinct brand-specific bacterial DNA profiles, conserved between IOLs and packaging fluid of all production lots within each manufacturer. The dominant taxonomy differentiating each manufacturer was annotated as Staphylococcus sp, and was a 100% match to S. epidermidis. Distinct mixtures of bacterial DNA are present and consistent in IOLs and packaging fluid depending on the manufacturer, and Staphylococcus is the dominant contributor to the bacterial DNA detected. Caralens products had a significantly lower amount of Staphylococcus spp. compared to Anvision and Dioptrix products.https://doi.org/10.1371/journal.pone.0277753 |
| spellingShingle | Kourtney K Dowler Aida Vientós-Plotts Elizabeth A Giuliano Zachary L McAdams Carol R Reinero Aaron C Ericsson Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. PLoS ONE |
| title | Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. |
| title_full | Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. |
| title_fullStr | Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. |
| title_full_unstemmed | Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. |
| title_short | Molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery. |
| title_sort | molecular and microbiological evidence of bacterial contamination of intraocular lenses commonly used in canine cataract surgery |
| url | https://doi.org/10.1371/journal.pone.0277753 |
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