DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity
The species diversity of freshwater fishes from the transboundary river, Jaldhaka is still unknown to the scientific communities. We generated 40 DNA barcode sequences of 16 morphologically identified freshwater fishes and compared genetically with the database sequences. Ten species (Acanthocobitis...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-07-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2019.1640079 |
| _version_ | 1797639713584054272 |
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| author | Shantanu Kundu Kaomud Tyagi Avas Pakrashi Vikas Kumar Laishram Kosygin Shibananda Rath Ujjal Das Kailash Chandra |
| author_facet | Shantanu Kundu Kaomud Tyagi Avas Pakrashi Vikas Kumar Laishram Kosygin Shibananda Rath Ujjal Das Kailash Chandra |
| author_sort | Shantanu Kundu |
| collection | DOAJ |
| description | The species diversity of freshwater fishes from the transboundary river, Jaldhaka is still unknown to the scientific communities. We generated 40 DNA barcode sequences of 16 morphologically identified freshwater fishes and compared genetically with the database sequences. Ten species (Acanthocobitis botia, Barilius bendelisis, Crossocheilus latius, Channa punctata, Channa quinquefasciata, Garra gotyla, Garra kempi, Opsarius barna, Psilorhynchus balitora, and Pseudecheneis sulcata) showed unique haplotypes in the studied riverine system. Further, the estimated genetic divergences, BA tree topology, and ABGD species delimitation methods revealed the presence of cryptic diversity in Badis badis, Garra annandalei, G. gotyla, G. kempi, P. balitora, Rasbora daniconius, and Pethia ticto. The study suggested more exhaustive sampling and generation of more molecular data to strengthen the fact. The aimed integrated approach will be helpful to detect the extant species diversity, helps to reevaluate the checklist and promote sustainable conservation management to protect this unparalleled ecosystem. |
| first_indexed | 2024-03-11T13:21:35Z |
| format | Article |
| id | doaj.art-6dddcec5a19e4518993ecad0a140e663 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:21:35Z |
| publishDate | 2019-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-6dddcec5a19e4518993ecad0a140e6632023-11-03T10:28:35ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592019-07-01422527253210.1080/23802359.2019.16400791640079DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversityShantanu Kundu0Kaomud Tyagi1Avas Pakrashi2Vikas Kumar3Laishram Kosygin4Shibananda Rath5Ujjal Das6Kailash Chandra7Zoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaThe species diversity of freshwater fishes from the transboundary river, Jaldhaka is still unknown to the scientific communities. We generated 40 DNA barcode sequences of 16 morphologically identified freshwater fishes and compared genetically with the database sequences. Ten species (Acanthocobitis botia, Barilius bendelisis, Crossocheilus latius, Channa punctata, Channa quinquefasciata, Garra gotyla, Garra kempi, Opsarius barna, Psilorhynchus balitora, and Pseudecheneis sulcata) showed unique haplotypes in the studied riverine system. Further, the estimated genetic divergences, BA tree topology, and ABGD species delimitation methods revealed the presence of cryptic diversity in Badis badis, Garra annandalei, G. gotyla, G. kempi, P. balitora, Rasbora daniconius, and Pethia ticto. The study suggested more exhaustive sampling and generation of more molecular data to strengthen the fact. The aimed integrated approach will be helpful to detect the extant species diversity, helps to reevaluate the checklist and promote sustainable conservation management to protect this unparalleled ecosystem.http://dx.doi.org/10.1080/23802359.2019.1640079ichthyofaunabiodiversity sharingdna barcodingcryptic speciesconservation |
| spellingShingle | Shantanu Kundu Kaomud Tyagi Avas Pakrashi Vikas Kumar Laishram Kosygin Shibananda Rath Ujjal Das Kailash Chandra DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity Mitochondrial DNA. Part B. Resources ichthyofauna biodiversity sharing dna barcoding cryptic species conservation |
| title | DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity |
| title_full | DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity |
| title_fullStr | DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity |
| title_full_unstemmed | DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity |
| title_short | DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity |
| title_sort | dna barcoding of freshwater fishes from the transboundary river of indo bhutan multiple clades and cryptic diversity |
| topic | ichthyofauna biodiversity sharing dna barcoding cryptic species conservation |
| url | http://dx.doi.org/10.1080/23802359.2019.1640079 |
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