Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease
Abstract Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 c...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02607-1 |
| _version_ | 1818995070734434304 |
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| author | Kazuki Heishima Yukie Ichikawa Kyoko Yoshida Ryota Iwasaki Hiroki Sakai Takayuki Nakagawa Yuiko Tanaka Yuki Hoshino Yasuhiko Okamura Mami Murakami Kohji Maruo Yukihiro Akao Takashi Mori |
| author_facet | Kazuki Heishima Yukie Ichikawa Kyoko Yoshida Ryota Iwasaki Hiroki Sakai Takayuki Nakagawa Yuiko Tanaka Yuki Hoshino Yasuhiko Okamura Mami Murakami Kohji Maruo Yukihiro Akao Takashi Mori |
| author_sort | Kazuki Heishima |
| collection | DOAJ |
| description | Abstract Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 cases of canine neoplastic diseases and healthy controls. MicroRNA-214 levels were high in 2 epithelial tumours (thyroid and mammary carcinomas) and 4 non-epithelial tumours (osteosarcoma, histiocytic sarcoma, chondrosarcoma, and hemangiosarcoma). In contrast, microRNA-126 levels were high in 6 epithelial tumours (mammary, hepatocellular, squamous cell, thyroid, transitional cell carcinomas, and adenocarcinoma) and 4 non-epithelial tumours (osteosarcoma, mast cell tumour, melanoma, and hemangiosarcoma). The diagnostic potential of microRNA-214 was relatively high in sarcomas, whereas that of microR-126 was high in most types of the tumours. MicroRNA-214 and -126 were prognostic predictors in 2 groups (adenocarcinoma and non-epithelial tumours except for osteosarcoma) and 3 groups (epithelial tumours, adenocarcinoma, and melanoma), respectively. Additionally, the microRNA levels did not show a strong correlation with the other clinical parameters. In conclusion, circulating microRNA-214 and -126 have the potential to be diagnostic and prognostic biomarkers for canine neoplastic diseases. Furthermore, their profiles may be key references as well for exploring novel biomarkers for human cancers. |
| first_indexed | 2024-12-20T21:08:00Z |
| format | Article |
| id | doaj.art-6ddeb2ca6aaf435bb2f9d64d897c1666 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:08:00Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6ddeb2ca6aaf435bb2f9d64d897c16662022-12-21T19:26:35ZengNature PortfolioScientific Reports2045-23222017-05-017111410.1038/s41598-017-02607-1Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic diseaseKazuki Heishima0Yukie Ichikawa1Kyoko Yoshida2Ryota Iwasaki3Hiroki Sakai4Takayuki Nakagawa5Yuiko Tanaka6Yuki Hoshino7Yasuhiko Okamura8Mami Murakami9Kohji Maruo10Yukihiro Akao11Takashi Mori12Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityDepartment of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityDepartment of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityDepartment of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityLaboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu UniversityLaboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of TokyoLaboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of TokyoVeterinary Teaching Hospital, Graduate School of Veterinary Medicine, Hokkaido UniversityCooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityDepartment of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu UniversityDepartment of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu UniversityAbstract Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 cases of canine neoplastic diseases and healthy controls. MicroRNA-214 levels were high in 2 epithelial tumours (thyroid and mammary carcinomas) and 4 non-epithelial tumours (osteosarcoma, histiocytic sarcoma, chondrosarcoma, and hemangiosarcoma). In contrast, microRNA-126 levels were high in 6 epithelial tumours (mammary, hepatocellular, squamous cell, thyroid, transitional cell carcinomas, and adenocarcinoma) and 4 non-epithelial tumours (osteosarcoma, mast cell tumour, melanoma, and hemangiosarcoma). The diagnostic potential of microRNA-214 was relatively high in sarcomas, whereas that of microR-126 was high in most types of the tumours. MicroRNA-214 and -126 were prognostic predictors in 2 groups (adenocarcinoma and non-epithelial tumours except for osteosarcoma) and 3 groups (epithelial tumours, adenocarcinoma, and melanoma), respectively. Additionally, the microRNA levels did not show a strong correlation with the other clinical parameters. In conclusion, circulating microRNA-214 and -126 have the potential to be diagnostic and prognostic biomarkers for canine neoplastic diseases. Furthermore, their profiles may be key references as well for exploring novel biomarkers for human cancers.https://doi.org/10.1038/s41598-017-02607-1 |
| spellingShingle | Kazuki Heishima Yukie Ichikawa Kyoko Yoshida Ryota Iwasaki Hiroki Sakai Takayuki Nakagawa Yuiko Tanaka Yuki Hoshino Yasuhiko Okamura Mami Murakami Kohji Maruo Yukihiro Akao Takashi Mori Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease Scientific Reports |
| title | Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease |
| title_full | Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease |
| title_fullStr | Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease |
| title_full_unstemmed | Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease |
| title_short | Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease |
| title_sort | circulating microrna 214 and 126 as potential biomarkers for canine neoplastic disease |
| url | https://doi.org/10.1038/s41598-017-02607-1 |
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