Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma
Soft tissue sarcomas are a large group of different tumor types both in humans and in animals. Among them, fibrosarcoma is the most frequent malignant mesenchymal tumoral form in cats, representing up to 28% of all cat skin tumors, while human fibrosarcoma, fortunately, only represents 5% of all sar...
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MDPI AG
2020-12-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/1/31 |
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| author | Rosa Patruno Giuseppe Passantino Carmelo Laface Antonella Tinelli Alfredo Zito Roberta Ruggieri Francesco Luposella Pietro Gadaleta Mariarita Laforgia Luca Lacitignola Michele Ammendola Girolamo Ranieri Nicola Zizzo |
| author_facet | Rosa Patruno Giuseppe Passantino Carmelo Laface Antonella Tinelli Alfredo Zito Roberta Ruggieri Francesco Luposella Pietro Gadaleta Mariarita Laforgia Luca Lacitignola Michele Ammendola Girolamo Ranieri Nicola Zizzo |
| author_sort | Rosa Patruno |
| collection | DOAJ |
| description | Soft tissue sarcomas are a large group of different tumor types both in humans and in animals. Among them, fibrosarcoma is the most frequent malignant mesenchymal tumoral form in cats, representing up to 28% of all cat skin tumors, while human fibrosarcoma, fortunately, only represents 5% of all sarcomas and 0.025% of the world-wide burden of tumors. This low incidence in humans leads to consideration of this group of tumoral diseases as rare, so therapeutic options are few due to the difficulty of starting clinical trials. In this context, the identification of research models for fibrosarcomas could be of great interest to deepen knowledge in this field and recognize new or possible biological pathways involved in tumor progression and metastasis. Angiogenesis is considered a fundamental scattering cause of tumor aggressiveness and progression in all forms of cancer, but only a few research parameters were developed and reported to express them quantitatively and qualitatively. The role in angiogenesis of microenvironmental stromal cells, such as fibroblasts, lymphocytes, mast cells, and macrophages, was largely demonstrated since this topic was first approached, while quantification of new vessels and their blood capacity in tumoral area is a relatively recent approach that could be well developed thanks to expertise in immunohistochemistry and image analysis. In this paper, a crossing study evaluating microvascular density (MVD), endothelial area (EA), and Ki-67 proliferative index was reported for a series of formalin-fixed and paraffin-embedded tissue samples from 99 cat patients, affected by cat post-injection fibrosarcoma, by using a till ×400 magnification light microscopy. We aim to demonstrate that cat pets may be considered a useful animal model for better studying the correspondent human diseases and we report, for the first time to our knowledge, experimental data in terms of correlation among MVD, EA, and Ki-67 strictly involved in aggressiveness and tumoral progression. |
| first_indexed | 2024-03-10T13:43:34Z |
| format | Article |
| id | doaj.art-6ddf6dad8bd647eda533e8bd81192de9 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T13:43:34Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-6ddf6dad8bd647eda533e8bd81192de92023-11-21T02:49:00ZengMDPI AGCells2073-44092020-12-011013110.3390/cells10010031Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection FibrosarcomaRosa Patruno0Giuseppe Passantino1Carmelo Laface2Antonella Tinelli3Alfredo Zito4Roberta Ruggieri5Francesco Luposella6Pietro Gadaleta7Mariarita Laforgia8Luca Lacitignola9Michele Ammendola10Girolamo Ranieri11Nicola Zizzo12Department of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari “Aldo Moro”, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, ItalyDepartment of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari “Aldo Moro”, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, ItalyInterventional and Medical Oncology Unit, IRCCS Istituto Tumori “G. Paolo II”, 70124 Bari, ItalyDepartment of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari “Aldo Moro”, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, ItalyPathology Unit, IRCCS Istituto Tumori “G. Paolo II”, 70124 Bari, ItalyCardiology Unit, University of Bari “Aldo Moro”, 70124 Bari, ItalyDirection Départementale de la Cohésion Sociale et de la Protection des Populations des VOSGES (DDCSPP88), 88080 Vittel, FranceInterventional and Medical Oncology Unit, IRCCS Istituto Tumori “G. Paolo II”, 70124 Bari, ItalyPharmacy Unit, IRCCS Istituto Tumori “G. Paolo II”, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation, University of Bari ‘Aldo Moro’, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, ItalyDepartment of Health Science, Digestive Surgery Unit, University “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, ItalyInterventional and Medical Oncology Unit, IRCCS Istituto Tumori “G. Paolo II”, 70124 Bari, ItalyDepartment of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari “Aldo Moro”, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, ItalySoft tissue sarcomas are a large group of different tumor types both in humans and in animals. Among them, fibrosarcoma is the most frequent malignant mesenchymal tumoral form in cats, representing up to 28% of all cat skin tumors, while human fibrosarcoma, fortunately, only represents 5% of all sarcomas and 0.025% of the world-wide burden of tumors. This low incidence in humans leads to consideration of this group of tumoral diseases as rare, so therapeutic options are few due to the difficulty of starting clinical trials. In this context, the identification of research models for fibrosarcomas could be of great interest to deepen knowledge in this field and recognize new or possible biological pathways involved in tumor progression and metastasis. Angiogenesis is considered a fundamental scattering cause of tumor aggressiveness and progression in all forms of cancer, but only a few research parameters were developed and reported to express them quantitatively and qualitatively. The role in angiogenesis of microenvironmental stromal cells, such as fibroblasts, lymphocytes, mast cells, and macrophages, was largely demonstrated since this topic was first approached, while quantification of new vessels and their blood capacity in tumoral area is a relatively recent approach that could be well developed thanks to expertise in immunohistochemistry and image analysis. In this paper, a crossing study evaluating microvascular density (MVD), endothelial area (EA), and Ki-67 proliferative index was reported for a series of formalin-fixed and paraffin-embedded tissue samples from 99 cat patients, affected by cat post-injection fibrosarcoma, by using a till ×400 magnification light microscopy. We aim to demonstrate that cat pets may be considered a useful animal model for better studying the correspondent human diseases and we report, for the first time to our knowledge, experimental data in terms of correlation among MVD, EA, and Ki-67 strictly involved in aggressiveness and tumoral progression.https://www.mdpi.com/2073-4409/10/1/31angiogenesiscat fibrosarcomaendothelial areaimmunohistochemistryKi-67 proliferation indexmicrovascular density |
| spellingShingle | Rosa Patruno Giuseppe Passantino Carmelo Laface Antonella Tinelli Alfredo Zito Roberta Ruggieri Francesco Luposella Pietro Gadaleta Mariarita Laforgia Luca Lacitignola Michele Ammendola Girolamo Ranieri Nicola Zizzo Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma Cells angiogenesis cat fibrosarcoma endothelial area immunohistochemistry Ki-67 proliferation index microvascular density |
| title | Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma |
| title_full | Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma |
| title_fullStr | Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma |
| title_full_unstemmed | Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma |
| title_short | Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma |
| title_sort | microvascular density endothelial area and ki 67 proliferative index correlate each other in cat post injection fibrosarcoma |
| topic | angiogenesis cat fibrosarcoma endothelial area immunohistochemistry Ki-67 proliferation index microvascular density |
| url | https://www.mdpi.com/2073-4409/10/1/31 |
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