Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo
Embryo transfer uses embryos developed in vivo or in vitro for cattle production, however there is a difference in the quality of the embryos obtained by the two methods. This study addresses the differences in gene expression between blastocysts developed in vitro and in vivo. In vivo blastocysts w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2019-11-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-100/_pdf/-char/en |
| _version_ | 1797615239869497344 |
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| author | Tatsuo NOGUCHI Takuro AIZAWA Yasuhisa MUNAKATA Hisataka IWATA |
| author_facet | Tatsuo NOGUCHI Takuro AIZAWA Yasuhisa MUNAKATA Hisataka IWATA |
| author_sort | Tatsuo NOGUCHI |
| collection | DOAJ |
| description | Embryo transfer uses embryos developed in vivo or in vitro for cattle production, however there is a difference in the quality of the embryos obtained by the two methods. This study addresses the differences in gene expression between blastocysts developed in vitro and in vivo. In vivo blastocysts were flushed from the uteri of super-ovulated cows and blastocysts developed in vitro were derived from in vitro matured and fertilized embryos. The same batch of frozen bull sperm was used for insemination and in vitro fertilization. Blastocysts were then subjected to RNA sequencing. Differentially expressed genes upregulated in in vitro blastocysts were annotated to focal adhesion, extracellular matrix (ECM)-receptor interaction, and PI3K-Akt signaling and the genes that were upregulated in in vivo blastocysts were annotated to oxidation-reduction processes, mitochondrion organization, and mitochondrial translation. Although the total cell number of the two types of blastocysts was similar, the mitochondrial quantity (determined by mitochondrial DNA copy numbers and expression levels of TOMM20), and ATP content in the blastocysts were lower in in vivo blastocysts compared with those developed in vitro. In conclusion, RNAseq revealed differential molecular backgrounds between in vitro and in vivo developed blastocysts and mitochondrial number and function are responsible for these differences. |
| first_indexed | 2024-03-11T07:23:35Z |
| format | Article |
| id | doaj.art-6ddfda17017b482eb8a56183ac261cd9 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:23:35Z |
| publishDate | 2019-11-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-6ddfda17017b482eb8a56183ac261cd92023-11-17T07:44:29ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002019-11-01661353910.1262/jrd.2019-100jrdComparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivoTatsuo NOGUCHI0Takuro AIZAWA1Yasuhisa MUNAKATA2Hisataka IWATA3Department of Animal Science, Tokyo University of Agriculture, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture, Kanagawa 243-0034, JapanEmbryo transfer uses embryos developed in vivo or in vitro for cattle production, however there is a difference in the quality of the embryos obtained by the two methods. This study addresses the differences in gene expression between blastocysts developed in vitro and in vivo. In vivo blastocysts were flushed from the uteri of super-ovulated cows and blastocysts developed in vitro were derived from in vitro matured and fertilized embryos. The same batch of frozen bull sperm was used for insemination and in vitro fertilization. Blastocysts were then subjected to RNA sequencing. Differentially expressed genes upregulated in in vitro blastocysts were annotated to focal adhesion, extracellular matrix (ECM)-receptor interaction, and PI3K-Akt signaling and the genes that were upregulated in in vivo blastocysts were annotated to oxidation-reduction processes, mitochondrion organization, and mitochondrial translation. Although the total cell number of the two types of blastocysts was similar, the mitochondrial quantity (determined by mitochondrial DNA copy numbers and expression levels of TOMM20), and ATP content in the blastocysts were lower in in vivo blastocysts compared with those developed in vitro. In conclusion, RNAseq revealed differential molecular backgrounds between in vitro and in vivo developed blastocysts and mitochondrial number and function are responsible for these differences.https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-100/_pdf/-char/enblastocystsgene expressionin vivoin vitromitochondria |
| spellingShingle | Tatsuo NOGUCHI Takuro AIZAWA Yasuhisa MUNAKATA Hisataka IWATA Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo The Journal of Reproduction and Development blastocysts gene expression in vivo in vitro mitochondria |
| title | Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| title_full | Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| title_fullStr | Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| title_full_unstemmed | Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| title_short | Comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| title_sort | comparison of gene expression and mitochondria number between bovine blastocysts obtained in vitro and in vivo |
| topic | blastocysts gene expression in vivo in vitro mitochondria |
| url | https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-100/_pdf/-char/en |
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