Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) develop at several anatomical locations and are thought to undergo different niche regulatory cues originating from highly conserved cell signaling pathways, such as Wnt, Notch, TGF-β family, and Hedgehog signaling. Most insight into these pathways has been obtained b...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/10/2264 |
| _version_ | 1797551485482958848 |
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| author | Jolanda. J.D. de Roo Frank. J.T. Staal |
| author_facet | Jolanda. J.D. de Roo Frank. J.T. Staal |
| author_sort | Jolanda. J.D. de Roo |
| collection | DOAJ |
| description | Hematopoietic stem cells (HSCs) develop at several anatomical locations and are thought to undergo different niche regulatory cues originating from highly conserved cell signaling pathways, such as Wnt, Notch, TGF-β family, and Hedgehog signaling. Most insight into these pathways has been obtained by reporter models and loss- or gain of function experiments, yet results differ in many cases according to the approach. In this review, we discuss existing murine reporter models regarding these pathways, considering the genetic constructs and reporter proteins in the context of HSC studies; yet these models are relevant for all other stem cell systems. Lastly, we describe a multi-reporter model to properly study and understand the cross-pathway interaction and how reporter models are highly valuable tools to understand complex signaling dynamics in stem cells. |
| first_indexed | 2024-03-10T15:45:46Z |
| format | Article |
| id | doaj.art-6de09757515e4833b2965c9f11ed6648 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T15:45:46Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-6de09757515e4833b2965c9f11ed66482023-11-20T16:28:57ZengMDPI AGCells2073-44092020-10-01910226410.3390/cells9102264Cell Signaling Pathway Reporters in Adult Hematopoietic Stem CellsJolanda. J.D. de Roo0Frank. J.T. Staal1Department of Immunology, L3-Q, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsDepartment of Immunology, L3-Q, Leiden University Medical Center, 2333 ZA Leiden, The NetherlandsHematopoietic stem cells (HSCs) develop at several anatomical locations and are thought to undergo different niche regulatory cues originating from highly conserved cell signaling pathways, such as Wnt, Notch, TGF-β family, and Hedgehog signaling. Most insight into these pathways has been obtained by reporter models and loss- or gain of function experiments, yet results differ in many cases according to the approach. In this review, we discuss existing murine reporter models regarding these pathways, considering the genetic constructs and reporter proteins in the context of HSC studies; yet these models are relevant for all other stem cell systems. Lastly, we describe a multi-reporter model to properly study and understand the cross-pathway interaction and how reporter models are highly valuable tools to understand complex signaling dynamics in stem cells.https://www.mdpi.com/2073-4409/9/10/2264murine reporter modelscell signaling pathwaysfluorescent reporter proteinshematopoietic stem cell biologymurine multi-reporter |
| spellingShingle | Jolanda. J.D. de Roo Frank. J.T. Staal Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells Cells murine reporter models cell signaling pathways fluorescent reporter proteins hematopoietic stem cell biology murine multi-reporter |
| title | Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells |
| title_full | Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells |
| title_fullStr | Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells |
| title_full_unstemmed | Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells |
| title_short | Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells |
| title_sort | cell signaling pathway reporters in adult hematopoietic stem cells |
| topic | murine reporter models cell signaling pathways fluorescent reporter proteins hematopoietic stem cell biology murine multi-reporter |
| url | https://www.mdpi.com/2073-4409/9/10/2264 |
| work_keys_str_mv | AT jolandajdderoo cellsignalingpathwayreportersinadulthematopoieticstemcells AT frankjtstaal cellsignalingpathwayreportersinadulthematopoieticstemcells |