iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii
As a common aquaculture pathogen, Citrobacter freundii has resulted in inconceivable economic losses in aquaculture. In this study, we investigated the spleen immune response of Lithobates catesbeianus to C. freundii infection at the protein level. A comparative proteomic analysis of spleens from C....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-04-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422000333 |
| _version_ | 1818776085941190656 |
|---|---|
| author | Peikui Yang Yuzhong Zheng Jude Juventus Aweya Xianghui Zou Min Lin Yaqun Liu Zhenxia Zhang Yanjie Sun Hongjie Wang |
| author_facet | Peikui Yang Yuzhong Zheng Jude Juventus Aweya Xianghui Zou Min Lin Yaqun Liu Zhenxia Zhang Yanjie Sun Hongjie Wang |
| author_sort | Peikui Yang |
| collection | DOAJ |
| description | As a common aquaculture pathogen, Citrobacter freundii has resulted in inconceivable economic losses in aquaculture. In this study, we investigated the spleen immune response of Lithobates catesbeianus to C. freundii infection at the protein level. A comparative proteomic analysis of spleens from C. freundii-infected L. catesbeianus was performed by using isobaric tags for relative and absolute quantitation (iTRAQ) approaches. A total of 2400 proteins were identified by LC-MS/MS spectra and sequencing data. In addition, 316 differentially expressed proteins (DEPs) were identified, of which 154 were significantly upregulated and 162 were significantly downregulated. Seven DEPs that are immune related including Kelch-like protein 22 (KLHL 22), saxiphilin (Sax), integrin beta-3 (ITGB3), retinoic acid-inducible gene I (RIG-I), laboratory of genetics and physiology 2 (LGP2), E3 ubiquitin-protein ligase TRIM39-like (TRIM39L), and phospholipid scramblase 1 (PLSCR1) were selected for further study. The expression profile of these genes in the spleen at 48 h post-C. freundii-infection showed a similar trend as the iTRAQ data. Altogether, the current study is the first to report the spleen proteome of bullfrogs (L. catesbeianus), providing insight into the immune response to C. freundii infection. |
| first_indexed | 2024-12-18T11:07:20Z |
| format | Article |
| id | doaj.art-6de26b685ef74d29897d57986ebaac42 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-18T11:07:20Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-6de26b685ef74d29897d57986ebaac422022-12-21T21:10:04ZengElsevierAquaculture Reports2352-51342022-04-0123101037iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundiiPeikui Yang0Yuzhong Zheng1Jude Juventus Aweya2Xianghui Zou3Min Lin4Yaqun Liu5Zhenxia Zhang6Yanjie Sun7Hongjie Wang8School of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, China; Corresponding authors.School of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, China; Corresponding authors.Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaSchool of Life Science and Food Engineering, Hanshan Normal University, Chaozhou 521041, ChinaAs a common aquaculture pathogen, Citrobacter freundii has resulted in inconceivable economic losses in aquaculture. In this study, we investigated the spleen immune response of Lithobates catesbeianus to C. freundii infection at the protein level. A comparative proteomic analysis of spleens from C. freundii-infected L. catesbeianus was performed by using isobaric tags for relative and absolute quantitation (iTRAQ) approaches. A total of 2400 proteins were identified by LC-MS/MS spectra and sequencing data. In addition, 316 differentially expressed proteins (DEPs) were identified, of which 154 were significantly upregulated and 162 were significantly downregulated. Seven DEPs that are immune related including Kelch-like protein 22 (KLHL 22), saxiphilin (Sax), integrin beta-3 (ITGB3), retinoic acid-inducible gene I (RIG-I), laboratory of genetics and physiology 2 (LGP2), E3 ubiquitin-protein ligase TRIM39-like (TRIM39L), and phospholipid scramblase 1 (PLSCR1) were selected for further study. The expression profile of these genes in the spleen at 48 h post-C. freundii-infection showed a similar trend as the iTRAQ data. Altogether, the current study is the first to report the spleen proteome of bullfrogs (L. catesbeianus), providing insight into the immune response to C. freundii infection.http://www.sciencedirect.com/science/article/pii/S2352513422000333Comparative proteomicsBullfrogLithobates catesbeianusCitrobacter freundiiiTRAQ |
| spellingShingle | Peikui Yang Yuzhong Zheng Jude Juventus Aweya Xianghui Zou Min Lin Yaqun Liu Zhenxia Zhang Yanjie Sun Hongjie Wang iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii Aquaculture Reports Comparative proteomics Bullfrog Lithobates catesbeianus Citrobacter freundii iTRAQ |
| title | iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii |
| title_full | iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii |
| title_fullStr | iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii |
| title_full_unstemmed | iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii |
| title_short | iTRAQ-based comparative proteomic analysis of the Lithobates catesbeianus bullfrog spleen following challenge with Citrobacter freundii |
| title_sort | itraq based comparative proteomic analysis of the lithobates catesbeianus bullfrog spleen following challenge with citrobacter freundii |
| topic | Comparative proteomics Bullfrog Lithobates catesbeianus Citrobacter freundii iTRAQ |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422000333 |
| work_keys_str_mv | AT peikuiyang itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT yuzhongzheng itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT judejuventusaweya itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT xianghuizou itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT minlin itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT yaqunliu itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT zhenxiazhang itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT yanjiesun itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii AT hongjiewang itraqbasedcomparativeproteomicanalysisofthelithobatescatesbeianusbullfrogspleenfollowingchallengewithcitrobacterfreundii |