Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer
Abstract Merkel cell polyomavirus (MCPyV) infects most people asymptomatically, but recent reports indicate that the virus may be related to carcinogenesis. This study aimed to evaluate the impact of MCPyV on the development of papillary thyroid cancer (PTC). Totally, 1057 samples, including 412 fre...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01055-2 |
| _version_ | 1828959888959602688 |
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| author | Monir Ghanghareh Jalal Mosayebi Amroabadi Seyed Mohammad Tavangar Shiva Irani Fatemeh Sakhaee Morteza Ghazanfari Jajin Farzam Vaziri Seyed Davar Siadat Abolfazl Fateh |
| author_facet | Monir Ghanghareh Jalal Mosayebi Amroabadi Seyed Mohammad Tavangar Shiva Irani Fatemeh Sakhaee Morteza Ghazanfari Jajin Farzam Vaziri Seyed Davar Siadat Abolfazl Fateh |
| author_sort | Monir Ghanghareh |
| collection | DOAJ |
| description | Abstract Merkel cell polyomavirus (MCPyV) infects most people asymptomatically, but recent reports indicate that the virus may be related to carcinogenesis. This study aimed to evaluate the impact of MCPyV on the development of papillary thyroid cancer (PTC). Totally, 1057 samples, including 412 fresh biopsy samples (FBS) and 645 paraffin-embedded PTC biopsy samples (PEBS), and 1057 adjacent non-cancerous samples were assessed for the presence of MCPyV DNA and RNA. MCPyV DNA was positive in 215 (20.3%) of samples, including 126 (30.6%) in FBS and 89 (13.8%) in PEBS. In MCPyV-positive samples, the mean MCPyV copy number was higher in the patients with FBS (2.3 × 10–1 ± 0.5 × 10–1 copies/cell) compared to PEBS (0.7 × 10–4 ± 0.1 × 10–4 copies/cell) and adjacent non-PTC normal samples (0.3 × 10–5 ± 0.02 × 10–5 copies/cell), indicating a statistically significant difference (P < 0.001). The LT-Ag RNA expression was higher in FBS compared to PEBS, while VP1 gene transcript was not detected in any samples. Although our findings showed the presence of MCPyV in a subset of PTC Iranian patients, further research is required to confirm these findings. |
| first_indexed | 2024-12-14T09:17:06Z |
| format | Article |
| id | doaj.art-6de36fab1d3947e5bc6db73bc89588bb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T09:17:06Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6de36fab1d3947e5bc6db73bc89588bb2022-12-21T23:08:26ZengNature PortfolioScientific Reports2045-23222021-11-011111610.1038/s41598-021-01055-2Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancerMonir Ghanghareh0Jalal Mosayebi Amroabadi1Seyed Mohammad Tavangar2Shiva Irani3Fatemeh Sakhaee4Morteza Ghazanfari Jajin5Farzam Vaziri6Seyed Davar Siadat7Abolfazl Fateh8Department of Biology, Science and Research Branch, Islamic Azad UniversityArtificial Intelligence and Multi-omics Center (AIMOC)Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical SciencesDepartment of Biology, Science and Research Branch, Islamic Azad UniversityDepartments of Mycobacteriology & Pulmonary Research, Pasteur Institute of IranDepartment of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical SciencesDepartments of Mycobacteriology & Pulmonary Research, Pasteur Institute of IranDepartments of Mycobacteriology & Pulmonary Research, Pasteur Institute of IranDepartments of Mycobacteriology & Pulmonary Research, Pasteur Institute of IranAbstract Merkel cell polyomavirus (MCPyV) infects most people asymptomatically, but recent reports indicate that the virus may be related to carcinogenesis. This study aimed to evaluate the impact of MCPyV on the development of papillary thyroid cancer (PTC). Totally, 1057 samples, including 412 fresh biopsy samples (FBS) and 645 paraffin-embedded PTC biopsy samples (PEBS), and 1057 adjacent non-cancerous samples were assessed for the presence of MCPyV DNA and RNA. MCPyV DNA was positive in 215 (20.3%) of samples, including 126 (30.6%) in FBS and 89 (13.8%) in PEBS. In MCPyV-positive samples, the mean MCPyV copy number was higher in the patients with FBS (2.3 × 10–1 ± 0.5 × 10–1 copies/cell) compared to PEBS (0.7 × 10–4 ± 0.1 × 10–4 copies/cell) and adjacent non-PTC normal samples (0.3 × 10–5 ± 0.02 × 10–5 copies/cell), indicating a statistically significant difference (P < 0.001). The LT-Ag RNA expression was higher in FBS compared to PEBS, while VP1 gene transcript was not detected in any samples. Although our findings showed the presence of MCPyV in a subset of PTC Iranian patients, further research is required to confirm these findings.https://doi.org/10.1038/s41598-021-01055-2 |
| spellingShingle | Monir Ghanghareh Jalal Mosayebi Amroabadi Seyed Mohammad Tavangar Shiva Irani Fatemeh Sakhaee Morteza Ghazanfari Jajin Farzam Vaziri Seyed Davar Siadat Abolfazl Fateh Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer Scientific Reports |
| title | Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| title_full | Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| title_fullStr | Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| title_full_unstemmed | Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| title_short | Evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| title_sort | evidencing the presence of merkel cell polyomavirus in papillary thyroid cancer |
| url | https://doi.org/10.1038/s41598-021-01055-2 |
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