Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform
High temperatures inhibit plant growth. A proposed strategy for improving plant productivity under elevated temperatures is the use of plant growth-promoting rhizobacteria (PGPR). While the effects of PGPR on plant shoots have been extensively explored, roots—particularly their spatial and temporal...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/21/2927 |
| _version_ | 1797466744590172160 |
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| author | Allene Macabuhay Borjana Arsova Michelle Watt Kerstin A. Nagel Henning Lenz Alexander Putz Sascha Adels Mark Müller-Linow Jana Kelm Alexander A. T. Johnson Robert Walker Gabriel Schaaf Ute Roessner |
| author_facet | Allene Macabuhay Borjana Arsova Michelle Watt Kerstin A. Nagel Henning Lenz Alexander Putz Sascha Adels Mark Müller-Linow Jana Kelm Alexander A. T. Johnson Robert Walker Gabriel Schaaf Ute Roessner |
| author_sort | Allene Macabuhay |
| collection | DOAJ |
| description | High temperatures inhibit plant growth. A proposed strategy for improving plant productivity under elevated temperatures is the use of plant growth-promoting rhizobacteria (PGPR). While the effects of PGPR on plant shoots have been extensively explored, roots—particularly their spatial and temporal dynamics—have been hard to study, due to their below-ground nature. Here, we characterized the time- and tissue-specific morphological changes in bacterized plants using a novel non-invasive high-resolution plant phenotyping and imaging platform—GrowScreen-Agar II. The platform uses custom-made agar plates, which allow air exchange to occur with the agar medium and enable the shoot to grow outside the compartment. The platform provides light protection to the roots, the exposure of it to the shoots, and the non-invasive phenotyping of both organs. <i>Arabidopsis thaliana</i>, co-cultivated with <i>Paraburkholderia phytofirmans</i> PsJN at elevated and ambient temperatures, showed increased lengths, growth rates, and numbers of roots. However, the magnitude and direction of the growth promotion varied depending on root type, timing, and temperature. The root length and distribution per depth and according to time was also influenced by bacterization and the temperature. The shoot biomass increased at the later stages under ambient temperature in the bacterized plants. The study offers insights into the timing of the tissue-specific, PsJN-induced morphological changes and should facilitate future molecular and biochemical studies on plant–microbe–environment interactions. |
| first_indexed | 2024-03-09T18:44:07Z |
| format | Article |
| id | doaj.art-6deac73bde3d4bf5b4f910cd4a129eec |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:44:07Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6deac73bde3d4bf5b4f910cd4a129eec2023-11-24T06:25:21ZengMDPI AGPlants2223-77472022-10-011121292710.3390/plants11212927Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping PlatformAllene Macabuhay0Borjana Arsova1Michelle Watt2Kerstin A. Nagel3Henning Lenz4Alexander Putz5Sascha Adels6Mark Müller-Linow7Jana Kelm8Alexander A. T. Johnson9Robert Walker10Gabriel Schaaf11Ute Roessner12School of BioSciences, University of Melbourne, Parkville, VIC 3010, AustraliaInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanySchool of BioSciences, University of Melbourne, Parkville, VIC 3010, AustraliaInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanyInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanyInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanyInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanyInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanyInstitute for Bio- & Geosciences (IBG-2), Plant Sciences, Forschungszentrum Juelich GmbH, 52425 Juelich, GermanySchool of BioSciences, University of Melbourne, Parkville, VIC 3010, AustraliaSchool of BioSciences, University of Melbourne, Parkville, VIC 3010, AustraliaInstitute of Crop Science and Resource Conservation, Department of Plant Nutrition, University of Bonn, 53115 Bonn, GermanySchool of BioSciences, University of Melbourne, Parkville, VIC 3010, AustraliaHigh temperatures inhibit plant growth. A proposed strategy for improving plant productivity under elevated temperatures is the use of plant growth-promoting rhizobacteria (PGPR). While the effects of PGPR on plant shoots have been extensively explored, roots—particularly their spatial and temporal dynamics—have been hard to study, due to their below-ground nature. Here, we characterized the time- and tissue-specific morphological changes in bacterized plants using a novel non-invasive high-resolution plant phenotyping and imaging platform—GrowScreen-Agar II. The platform uses custom-made agar plates, which allow air exchange to occur with the agar medium and enable the shoot to grow outside the compartment. The platform provides light protection to the roots, the exposure of it to the shoots, and the non-invasive phenotyping of both organs. <i>Arabidopsis thaliana</i>, co-cultivated with <i>Paraburkholderia phytofirmans</i> PsJN at elevated and ambient temperatures, showed increased lengths, growth rates, and numbers of roots. However, the magnitude and direction of the growth promotion varied depending on root type, timing, and temperature. The root length and distribution per depth and according to time was also influenced by bacterization and the temperature. The shoot biomass increased at the later stages under ambient temperature in the bacterized plants. The study offers insights into the timing of the tissue-specific, PsJN-induced morphological changes and should facilitate future molecular and biochemical studies on plant–microbe–environment interactions.https://www.mdpi.com/2223-7747/11/21/2927plant growth-promoting rhizobacteria (PGPR)<i>Paraburkholderia phytofirmans</i> PsJNphenotypingroot morphologyroot system architecturehigh temperature |
| spellingShingle | Allene Macabuhay Borjana Arsova Michelle Watt Kerstin A. Nagel Henning Lenz Alexander Putz Sascha Adels Mark Müller-Linow Jana Kelm Alexander A. T. Johnson Robert Walker Gabriel Schaaf Ute Roessner Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform Plants plant growth-promoting rhizobacteria (PGPR) <i>Paraburkholderia phytofirmans</i> PsJN phenotyping root morphology root system architecture high temperature |
| title | Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform |
| title_full | Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform |
| title_fullStr | Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform |
| title_full_unstemmed | Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform |
| title_short | Plant Growth Promotion and Heat Stress Amelioration in Arabidopsis Inoculated with <i>Paraburkholderia phytofirmans</i> PsJN Rhizobacteria Quantified with the GrowScreen-Agar II Phenotyping Platform |
| title_sort | plant growth promotion and heat stress amelioration in arabidopsis inoculated with i paraburkholderia phytofirmans i psjn rhizobacteria quantified with the growscreen agar ii phenotyping platform |
| topic | plant growth-promoting rhizobacteria (PGPR) <i>Paraburkholderia phytofirmans</i> PsJN phenotyping root morphology root system architecture high temperature |
| url | https://www.mdpi.com/2223-7747/11/21/2927 |
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