Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts
Polyinosinic:polycytidylic acid or poly(I:C) is a double stranded RNA analog that is known for stimulating RNA sensing pathways in a variety of cell culture and animal models. Activation of nucleic acid sensing pathways lead to the up-regulation of Interferon-β which ultimately creates an antiviral...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Veterinary Vaccine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772535923000045 |
| _version_ | 1797235466902175744 |
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| author | Jatinder Singh Chera Sushil Kumar Amit Kumar Bairagi Amit Kumar Prakash Chandra Ashutosh Vats Murtaza Ali Mayank Roshan Ranjit Singh Kataria Sachinandan De |
| author_facet | Jatinder Singh Chera Sushil Kumar Amit Kumar Bairagi Amit Kumar Prakash Chandra Ashutosh Vats Murtaza Ali Mayank Roshan Ranjit Singh Kataria Sachinandan De |
| author_sort | Jatinder Singh Chera |
| collection | DOAJ |
| description | Polyinosinic:polycytidylic acid or poly(I:C) is a double stranded RNA analog that is known for stimulating RNA sensing pathways in a variety of cell culture and animal models. Activation of nucleic acid sensing pathways lead to the up-regulation of Interferon-β which ultimately creates an antiviral state in the host. The involvement of intracellular DNA sensors in poly(I:C) mediated immune response has not been extensively explored. Poly(I:C) stimulates the intracellular DNA sensing in addition to RNA sensing pathways in buffalo fibroblasts. Genomic DNA leak into the cytosol due to poly(I:C) is likely to stimulate the intracellular DNA sensing pathways. The mRNA expression of DNA sensors cGAS, IFI16-L (IFI16-like) and DAI are elevated while DDX41 and STING are down-regulated in poly(I:C) treated cells. However, STING activation is evident in poly(I:C) treated cells through the formation of aggregates around the nucleus. IFI16-L also aggregates like STING and translocates from the nucleus to the cytoplasm in response to poly(I:C). The activation of DNA sensors by a dsRNA mimic indicates that mammalian cells can use their own molecules for defending themselves against pathogenic RNA viruses. This provides an opportunity to search for novel therapeutic targets against RNA viruses that evade detection by canonical pattern recognition receptors. |
| first_indexed | 2024-04-24T16:48:25Z |
| format | Article |
| id | doaj.art-6deee7546fb4406491ab081ca08353fb |
| institution | Directory Open Access Journal |
| issn | 2772-5359 |
| language | English |
| last_indexed | 2024-04-24T16:48:25Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Veterinary Vaccine |
| spelling | doaj.art-6deee7546fb4406491ab081ca08353fb2024-03-29T05:51:25ZengElsevierVeterinary Vaccine2772-53592023-03-0121100016Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblastsJatinder Singh Chera0Sushil Kumar1Amit Kumar Bairagi2Amit Kumar3Prakash Chandra4Ashutosh Vats5Murtaza Ali6Mayank Roshan7Ranjit Singh Kataria8Sachinandan De9Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaDivision of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, IndiaAnimal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, India; Corresponding author.Polyinosinic:polycytidylic acid or poly(I:C) is a double stranded RNA analog that is known for stimulating RNA sensing pathways in a variety of cell culture and animal models. Activation of nucleic acid sensing pathways lead to the up-regulation of Interferon-β which ultimately creates an antiviral state in the host. The involvement of intracellular DNA sensors in poly(I:C) mediated immune response has not been extensively explored. Poly(I:C) stimulates the intracellular DNA sensing in addition to RNA sensing pathways in buffalo fibroblasts. Genomic DNA leak into the cytosol due to poly(I:C) is likely to stimulate the intracellular DNA sensing pathways. The mRNA expression of DNA sensors cGAS, IFI16-L (IFI16-like) and DAI are elevated while DDX41 and STING are down-regulated in poly(I:C) treated cells. However, STING activation is evident in poly(I:C) treated cells through the formation of aggregates around the nucleus. IFI16-L also aggregates like STING and translocates from the nucleus to the cytoplasm in response to poly(I:C). The activation of DNA sensors by a dsRNA mimic indicates that mammalian cells can use their own molecules for defending themselves against pathogenic RNA viruses. This provides an opportunity to search for novel therapeutic targets against RNA viruses that evade detection by canonical pattern recognition receptors.http://www.sciencedirect.com/science/article/pii/S2772535923000045InterferonInnateFibroblastImmunityAntiviralPoly(I:C) |
| spellingShingle | Jatinder Singh Chera Sushil Kumar Amit Kumar Bairagi Amit Kumar Prakash Chandra Ashutosh Vats Murtaza Ali Mayank Roshan Ranjit Singh Kataria Sachinandan De Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts Veterinary Vaccine Interferon Innate Fibroblast Immunity Antiviral Poly(I:C) |
| title | Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts |
| title_full | Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts |
| title_fullStr | Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts |
| title_full_unstemmed | Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts |
| title_short | Poly(I:C), a double stranded RNA analog, activates the anti-viral DNA sensors in buffalo fibroblasts |
| title_sort | poly i c a double stranded rna analog activates the anti viral dna sensors in buffalo fibroblasts |
| topic | Interferon Innate Fibroblast Immunity Antiviral Poly(I:C) |
| url | http://www.sciencedirect.com/science/article/pii/S2772535923000045 |
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