Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner
Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection thr...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2016-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0195-R |
| _version_ | 1811291385727287296 |
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| author | Sebastián Acosta-Jurado Dulce-Nombre Rodríguez-Navarro Yasuyuki Kawaharada Juan Fernández Perea Antonio Gil-Serrano Haojie Jin Qi An Miguel A. Rodríguez-Carvajal Stig U. Andersen Niels Sandal Jens Stougaard José-María Vinardell José E. Ruiz-Sainz |
| author_facet | Sebastián Acosta-Jurado Dulce-Nombre Rodríguez-Navarro Yasuyuki Kawaharada Juan Fernández Perea Antonio Gil-Serrano Haojie Jin Qi An Miguel A. Rodríguez-Carvajal Stig U. Andersen Niels Sandal Jens Stougaard José-María Vinardell José E. Ruiz-Sainz |
| author_sort | Sebastián Acosta-Jurado |
| collection | DOAJ |
| description | Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rifr surface-polysaccharide mutants gained effective nodulation with L. japonicus. |
| first_indexed | 2024-04-13T04:28:35Z |
| format | Article |
| id | doaj.art-6df1f3d56b714fc59d6f3e787e755999 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:28:35Z |
| publishDate | 2016-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6df1f3d56b714fc59d6f3e787e7559992022-12-22T03:02:25ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062016-12-01291292593710.1094/MPMI-09-16-0195-RSinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent MannerSebastián Acosta-Jurado0Dulce-Nombre Rodríguez-Navarro1Yasuyuki Kawaharada2Juan Fernández Perea3Antonio Gil-Serrano4Haojie Jin5Qi An6Miguel A. Rodríguez-Carvajal7Stig U. Andersen8Niels Sandal9Jens Stougaard10José-María Vinardell11José E. Ruiz-Sainz12Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, C.P. 41012, Sevilla, Spain;IFAPA, Centro Las Torres-Tomejil, Apartado Oficial 41200, Alcalá del Río, Sevilla, Spain;Department of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wieds Vej 10, Aarhus C DK-8000, Denmark; andIFAPA, Centro Las Torres-Tomejil, Apartado Oficial 41200, Alcalá del Río, Sevilla, Spain;Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Calle Profesor García González 1, C. P. 41012, Sevilla, SpainDepartment of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wieds Vej 10, Aarhus C DK-8000, Denmark; andDepartamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, C.P. 41012, Sevilla, Spain;Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Calle Profesor García González 1, C. P. 41012, Sevilla, SpainDepartment of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wieds Vej 10, Aarhus C DK-8000, Denmark; andDepartment of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wieds Vej 10, Aarhus C DK-8000, Denmark; andDepartment of Molecular Biology and Genetics, Centre for Carbohydrate Recognition and Signalling, Aarhus University, Gustav Wieds Vej 10, Aarhus C DK-8000, Denmark; andDepartamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, C.P. 41012, Sevilla, Spain;Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, C.P. 41012, Sevilla, Spain;Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rifr surface-polysaccharide mutants gained effective nodulation with L. japonicus.https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0195-R |
| spellingShingle | Sebastián Acosta-Jurado Dulce-Nombre Rodríguez-Navarro Yasuyuki Kawaharada Juan Fernández Perea Antonio Gil-Serrano Haojie Jin Qi An Miguel A. Rodríguez-Carvajal Stig U. Andersen Niels Sandal Jens Stougaard José-María Vinardell José E. Ruiz-Sainz Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner Molecular Plant-Microbe Interactions |
| title | Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner |
| title_full | Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner |
| title_fullStr | Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner |
| title_full_unstemmed | Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner |
| title_short | Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner |
| title_sort | sinorhizobium fredii hh103 invades lotus burttii by crack entry in a nod factor and surface polysaccharide dependent manner |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-09-16-0195-R |
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