Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop
The vegetative propagation of hops, despite being a reliable method, is not very common due to the unavailability of the plant material. In this study, the technique of in vitro propagation was applied to the Cascade variety of <i>Humulus lupulus</i> L. The plant material was collected f...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/15/2877 |
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| author | Nicolò Iacuzzi Francesco Salamone Davide Farruggia Noemi Tortorici Lorena Vultaggio Teresa Tuttolomondo |
| author_facet | Nicolò Iacuzzi Francesco Salamone Davide Farruggia Noemi Tortorici Lorena Vultaggio Teresa Tuttolomondo |
| author_sort | Nicolò Iacuzzi |
| collection | DOAJ |
| description | The vegetative propagation of hops, despite being a reliable method, is not very common due to the unavailability of the plant material. In this study, the technique of in vitro propagation was applied to the Cascade variety of <i>Humulus lupulus</i> L. The plant material was collected from a private field in Sicily; the explants were subjected to sterilization before in vitro culture. Single-node explants were placed in in vitro culture in nine different culture media for multiplication. Thidiazuron (TDZ), Benzyladenine (BAP) and meta-Topoline (mT) were tested for multiplication phase. For the rooting phase, five types of different culture media were evaluated. Binodal cuttings coming from the previous multiplication test were placed in the culture. The rooting media differ from each other in the concentration and ratio of two auxin hormones: Indolo-3-acetic acid (IAA) and Indole-3-butyric acid (IBA). In vitro rooted plants obtained from the rooting phase were transferred to ex vitro conditions in a microbox with agri-perlite and a solution containing Murashige and Skoog (MS) basal medium at half concentration. With a culture medium containing the highest TDZ doses (H6) and combination with cytokinin (H8 and H9), the highest shoot percentage was obtained. After 3 months of in vitro culture, the highest shoot percentage was observed in the culture medium with 2 mL L<sup>−1</sup> of BAP. The highest rooting percentage, roots numbers and root length were found when the culture medium was supplemented with 1 mL L<sup>−1</sup> of IAA. The usage of agri-perlite and MS at half concentration, without PGR, allowed us to obtain a 99.1% survival rate. This micropropagation protocol is useful for obtaining virus-free plants and for the development of the brewery industry. |
| first_indexed | 2024-03-11T00:18:26Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:18:26Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6df3cfb0402a409c9bcd998ea751ffbf2023-11-18T23:27:06ZengMDPI AGPlants2223-77472023-08-011215287710.3390/plants12152877Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade HopNicolò Iacuzzi0Francesco Salamone1Davide Farruggia2Noemi Tortorici3Lorena Vultaggio4Teresa Tuttolomondo5Department of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyDepartment of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, ItalyThe vegetative propagation of hops, despite being a reliable method, is not very common due to the unavailability of the plant material. In this study, the technique of in vitro propagation was applied to the Cascade variety of <i>Humulus lupulus</i> L. The plant material was collected from a private field in Sicily; the explants were subjected to sterilization before in vitro culture. Single-node explants were placed in in vitro culture in nine different culture media for multiplication. Thidiazuron (TDZ), Benzyladenine (BAP) and meta-Topoline (mT) were tested for multiplication phase. For the rooting phase, five types of different culture media were evaluated. Binodal cuttings coming from the previous multiplication test were placed in the culture. The rooting media differ from each other in the concentration and ratio of two auxin hormones: Indolo-3-acetic acid (IAA) and Indole-3-butyric acid (IBA). In vitro rooted plants obtained from the rooting phase were transferred to ex vitro conditions in a microbox with agri-perlite and a solution containing Murashige and Skoog (MS) basal medium at half concentration. With a culture medium containing the highest TDZ doses (H6) and combination with cytokinin (H8 and H9), the highest shoot percentage was obtained. After 3 months of in vitro culture, the highest shoot percentage was observed in the culture medium with 2 mL L<sup>−1</sup> of BAP. The highest rooting percentage, roots numbers and root length were found when the culture medium was supplemented with 1 mL L<sup>−1</sup> of IAA. The usage of agri-perlite and MS at half concentration, without PGR, allowed us to obtain a 99.1% survival rate. This micropropagation protocol is useful for obtaining virus-free plants and for the development of the brewery industry.https://www.mdpi.com/2223-7747/12/15/2877<i>Humulus lupulus</i>micropropagationsingle-node explantscytokininauxins |
| spellingShingle | Nicolò Iacuzzi Francesco Salamone Davide Farruggia Noemi Tortorici Lorena Vultaggio Teresa Tuttolomondo Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop Plants <i>Humulus lupulus</i> micropropagation single-node explants cytokinin auxins |
| title | Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop |
| title_full | Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop |
| title_fullStr | Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop |
| title_full_unstemmed | Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop |
| title_short | Development of a New Micropropagation Protocol and Transfer of In Vitro Plants to In Vivo Conditions for Cascade Hop |
| title_sort | development of a new micropropagation protocol and transfer of in vitro plants to in vivo conditions for cascade hop |
| topic | <i>Humulus lupulus</i> micropropagation single-node explants cytokinin auxins |
| url | https://www.mdpi.com/2223-7747/12/15/2877 |
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