Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018.
The relatively high frequency of marine mammal stranding events in the Philippines provide many research opportunities. A select set of stranders (n = 21) from 2017 to 2018 were sampled for bacteriology and histopathology. Pertinent tissues and bacteria were collected from individuals representing e...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0243691 |
| _version_ | 1819098498736324608 |
|---|---|
| author | Marie Christine M Obusan Jamaica Ann A Caras Lara Sabrina L Lumang Erika Joyce S Calderon Ren Mark D Villanueva Cristina C Salibay Maria Auxilia T Siringan Windell L Rivera Joseph S Masangkay Lemnuel V Aragones |
| author_facet | Marie Christine M Obusan Jamaica Ann A Caras Lara Sabrina L Lumang Erika Joyce S Calderon Ren Mark D Villanueva Cristina C Salibay Maria Auxilia T Siringan Windell L Rivera Joseph S Masangkay Lemnuel V Aragones |
| author_sort | Marie Christine M Obusan |
| collection | DOAJ |
| description | The relatively high frequency of marine mammal stranding events in the Philippines provide many research opportunities. A select set of stranders (n = 21) from 2017 to 2018 were sampled for bacteriology and histopathology. Pertinent tissues and bacteria were collected from individuals representing eight cetacean species (i.e. Feresa attenuata, Kogia breviceps, Globicephala macrorhynchus, Grampus griseus, Lagenodelphis hosei, Peponocephala electra, Stenella attenuata and Stenella longirostris) and were subjected to histopathological examination and antibiotic resistance screening, respectively. The antibiotic resistance profiles of 24 bacteria (belonging to genera Escherichia, Enterobacter, Klebsiella, Proteus, and Shigella) that were isolated from four cetaceans were determined using 18 antibiotics. All 24 isolates were resistant to at least one antibiotic class, and 79.17% were classified as multiple antibiotic resistant (MAR). The MAR index values of isolates ranged from 0.06 to 0.39 with all the isolates resistant to erythromycin (100%; n = 24) and susceptible to imipenem, doripenem, ciprofloxacin, chloramphenicol, and gentamicin (100%; n = 24). The resistance profiles of these bacteria show the extent of antimicrobial resistance in the marine environment, and may inform medical management decisions during rehabilitation of stranded cetaceans. Due to inadequate gross descriptions and limited data gathered by the responders during the stranding events, the significance of histopathological lesions in association with disease diagnosis in each cetacean stranding or mortality remained inconclusive; however, these histopathological findings may be indicative or contributory to the resulting debility and stress during their strandings. The findings of the study demonstrate the challenges faced by cetacean species in the wild, such as but not limited to, biological pollution through land-sea movement of effluents, fisheries interactions, and anthropogenic activities. |
| first_indexed | 2024-12-22T00:31:57Z |
| format | Article |
| id | doaj.art-6df5c082a00f4f95b6f28b7b74e57979 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T00:31:57Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6df5c082a00f4f95b6f28b7b74e579792022-12-21T18:44:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011611e024369110.1371/journal.pone.0243691Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018.Marie Christine M ObusanJamaica Ann A CarasLara Sabrina L LumangErika Joyce S CalderonRen Mark D VillanuevaCristina C SalibayMaria Auxilia T SiringanWindell L RiveraJoseph S MasangkayLemnuel V AragonesThe relatively high frequency of marine mammal stranding events in the Philippines provide many research opportunities. A select set of stranders (n = 21) from 2017 to 2018 were sampled for bacteriology and histopathology. Pertinent tissues and bacteria were collected from individuals representing eight cetacean species (i.e. Feresa attenuata, Kogia breviceps, Globicephala macrorhynchus, Grampus griseus, Lagenodelphis hosei, Peponocephala electra, Stenella attenuata and Stenella longirostris) and were subjected to histopathological examination and antibiotic resistance screening, respectively. The antibiotic resistance profiles of 24 bacteria (belonging to genera Escherichia, Enterobacter, Klebsiella, Proteus, and Shigella) that were isolated from four cetaceans were determined using 18 antibiotics. All 24 isolates were resistant to at least one antibiotic class, and 79.17% were classified as multiple antibiotic resistant (MAR). The MAR index values of isolates ranged from 0.06 to 0.39 with all the isolates resistant to erythromycin (100%; n = 24) and susceptible to imipenem, doripenem, ciprofloxacin, chloramphenicol, and gentamicin (100%; n = 24). The resistance profiles of these bacteria show the extent of antimicrobial resistance in the marine environment, and may inform medical management decisions during rehabilitation of stranded cetaceans. Due to inadequate gross descriptions and limited data gathered by the responders during the stranding events, the significance of histopathological lesions in association with disease diagnosis in each cetacean stranding or mortality remained inconclusive; however, these histopathological findings may be indicative or contributory to the resulting debility and stress during their strandings. The findings of the study demonstrate the challenges faced by cetacean species in the wild, such as but not limited to, biological pollution through land-sea movement of effluents, fisheries interactions, and anthropogenic activities.https://doi.org/10.1371/journal.pone.0243691 |
| spellingShingle | Marie Christine M Obusan Jamaica Ann A Caras Lara Sabrina L Lumang Erika Joyce S Calderon Ren Mark D Villanueva Cristina C Salibay Maria Auxilia T Siringan Windell L Rivera Joseph S Masangkay Lemnuel V Aragones Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. PLoS ONE |
| title | Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. |
| title_full | Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. |
| title_fullStr | Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. |
| title_full_unstemmed | Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. |
| title_short | Bacteriological and histopathological findings in cetaceans that stranded in the Philippines from 2017 to 2018. |
| title_sort | bacteriological and histopathological findings in cetaceans that stranded in the philippines from 2017 to 2018 |
| url | https://doi.org/10.1371/journal.pone.0243691 |
| work_keys_str_mv | AT mariechristinemobusan bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT jamaicaannacaras bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT larasabrinallumang bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT erikajoycescalderon bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT renmarkdvillanueva bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT cristinacsalibay bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT mariaauxiliatsiringan bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT windelllrivera bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT josephsmasangkay bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 AT lemnuelvaragones bacteriologicalandhistopathologicalfindingsincetaceansthatstrandedinthephilippinesfrom2017to2018 |