Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis
Background: In Japan, pollinosis caused by the Japanese cypress (JCy) Chamaecyparis obtusa is among the very common seasonal allergies. In JCy pollinosis, Cha o 1 is the first major allergen, and Cha o 2 is the second major allergen. Recently, Cha o 3 was identified as a new JCy pollen allergen in J...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Journal of Allergy and Clinical Immunology: Global |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772829323001236 |
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| author | Yoshiaki Kobayashi, MD Keisuke Suzuki, MSc Minoru Tateno, PhD Yuki Nakamura, PhD Kayoko Ishimaru Yuka Nagasaka Daiju Sakurai, MD, PhD Katsuyo Ohashi-Doi, PhD Atsuhito Nakao, MD, PhD |
| author_facet | Yoshiaki Kobayashi, MD Keisuke Suzuki, MSc Minoru Tateno, PhD Yuki Nakamura, PhD Kayoko Ishimaru Yuka Nagasaka Daiju Sakurai, MD, PhD Katsuyo Ohashi-Doi, PhD Atsuhito Nakao, MD, PhD |
| author_sort | Yoshiaki Kobayashi, MD |
| collection | DOAJ |
| description | Background: In Japan, pollinosis caused by the Japanese cypress (JCy) Chamaecyparis obtusa is among the very common seasonal allergies. In JCy pollinosis, Cha o 1 is the first major allergen, and Cha o 2 is the second major allergen. Recently, Cha o 3 was identified as a new JCy pollen allergen in JCy pollinosis. However, the relative contribution of Cha o 3 to JCy pollinosis compared with that of Cha o 1 and that of Cha o 2 has not been fully elucidated. Objective: This study aimed to clarify the allergenicity of Cha o 3 compared with that of Cha o 1 and Cha o 2 in JCy pollinosis. Methods: We recruited 27 patients with JCy pollinosis and performed the basophil activation test (BAT) with native (n) Cha o 1, Cha o 2, and Cha o 3 purified from JCy pollen. In addition, we a performed JCy-specific IgE suppression test. Results: In the BAT, 26 of 27 patients (96%) and 18 of 27 patients (67%) showed positive basophil activation in response to n Cha o 1 and n Cha o 2, respectively, as judged by CD203c expression. Little CD203c expression in response to n Cha o 3 was seen. The presence of n Cha o 3 marginally reduced the titer levels of JCy-specific IgE. Conclusion: Cha o 3 showed little ability to activate basophils and suppress JCy-specific IgE titers compared with Cha o 1 or Cha o 2 in patients with JCy pollinosis. Thus, Cha o 3 may not be a major allergen in JCy pollinosis. |
| first_indexed | 2024-03-08T03:29:21Z |
| format | Article |
| id | doaj.art-6df5c98618d94ef0b6a99fe1f43d8ca0 |
| institution | Directory Open Access Journal |
| issn | 2772-8293 |
| language | English |
| last_indexed | 2024-03-08T03:29:21Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Allergy and Clinical Immunology: Global |
| spelling | doaj.art-6df5c98618d94ef0b6a99fe1f43d8ca02024-02-11T05:13:09ZengElsevierJournal of Allergy and Clinical Immunology: Global2772-82932024-02-0131100198Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosisYoshiaki Kobayashi, MD0Keisuke Suzuki, MSc1Minoru Tateno, PhD2Yuki Nakamura, PhD3Kayoko Ishimaru4Yuka Nagasaka5Daiju Sakurai, MD, PhD6Katsuyo Ohashi-Doi, PhD7Atsuhito Nakao, MD, PhD8Department of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, JapanTorii Pharmaceutical Co, Ltd, Tokyo, JapanTorii Pharmaceutical Co, Ltd, Tokyo, JapanDepartment of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, JapanDepartment of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, JapanDepartment of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, JapanDepartment of Otorhinolaryngology, Head and Neck Surgery, University of Yamanashi, Yamanashi, JapanTorii Pharmaceutical Co, Ltd, Tokyo, Japan; Corresponding author: Katsuyo Ohashi-Doi, PhD, Medical Affair Department, Torii Pharmaceutical Co, Ltd, 3-4-1, Nihonbashi-Honcho, Chuo-ku, Tokyo, 103-8439, Japan.Department of Immunology, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan; Yamanashi GLIA Center, University of Yamanashi, Yamanashi, Japan; Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan; Atsuhito Nakao, MD, PhD, Department of Immunology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3821, Japan.Background: In Japan, pollinosis caused by the Japanese cypress (JCy) Chamaecyparis obtusa is among the very common seasonal allergies. In JCy pollinosis, Cha o 1 is the first major allergen, and Cha o 2 is the second major allergen. Recently, Cha o 3 was identified as a new JCy pollen allergen in JCy pollinosis. However, the relative contribution of Cha o 3 to JCy pollinosis compared with that of Cha o 1 and that of Cha o 2 has not been fully elucidated. Objective: This study aimed to clarify the allergenicity of Cha o 3 compared with that of Cha o 1 and Cha o 2 in JCy pollinosis. Methods: We recruited 27 patients with JCy pollinosis and performed the basophil activation test (BAT) with native (n) Cha o 1, Cha o 2, and Cha o 3 purified from JCy pollen. In addition, we a performed JCy-specific IgE suppression test. Results: In the BAT, 26 of 27 patients (96%) and 18 of 27 patients (67%) showed positive basophil activation in response to n Cha o 1 and n Cha o 2, respectively, as judged by CD203c expression. Little CD203c expression in response to n Cha o 3 was seen. The presence of n Cha o 3 marginally reduced the titer levels of JCy-specific IgE. Conclusion: Cha o 3 showed little ability to activate basophils and suppress JCy-specific IgE titers compared with Cha o 1 or Cha o 2 in patients with JCy pollinosis. Thus, Cha o 3 may not be a major allergen in JCy pollinosis.http://www.sciencedirect.com/science/article/pii/S2772829323001236Japanese cypress pollinosisCha o 3basophil activation test |
| spellingShingle | Yoshiaki Kobayashi, MD Keisuke Suzuki, MSc Minoru Tateno, PhD Yuki Nakamura, PhD Kayoko Ishimaru Yuka Nagasaka Daiju Sakurai, MD, PhD Katsuyo Ohashi-Doi, PhD Atsuhito Nakao, MD, PhD Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis Journal of Allergy and Clinical Immunology: Global Japanese cypress pollinosis Cha o 3 basophil activation test |
| title | Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis |
| title_full | Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis |
| title_fullStr | Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis |
| title_full_unstemmed | Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis |
| title_short | Cha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis |
| title_sort | cha o 3 a cypress pollen allergen does not activate basophils in japanese cypress pollinosis |
| topic | Japanese cypress pollinosis Cha o 3 basophil activation test |
| url | http://www.sciencedirect.com/science/article/pii/S2772829323001236 |
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