<i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i>
While the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer <i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/7/421 |
| _version_ | 1811299475988152320 |
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| author | Gorenka Bojadzija Savic Christine Edwards Enora Briand Linda Lawton Claudia Wiegand Myriam Bormans |
| author_facet | Gorenka Bojadzija Savic Christine Edwards Enora Briand Linda Lawton Claudia Wiegand Myriam Bormans |
| author_sort | Gorenka Bojadzija Savic |
| collection | DOAJ |
| description | While the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer <i>Daphnia</i> reduce cyanobacterial dominance until a certain density, which may be supported by <i>Daphnia</i> exudates, affecting the cyanobacterial physiological state and metabolites’ production. Therefore, we hypothesized that <i>D. magna</i> spent medium will impact the production of cyanobacterial bioactive metabolites and affect cyanobacterial photosynthetic activity in the nontoxic, but not the toxic strain. Microcystin (MC-LR and des-MC-LR) producing <i>M. aeruginosa</i> PCC7806 and its non-microcystin producing mutant were exposed to spent media of different <i>D. magna</i> densities and culture durations. <i>D. magna</i> spent medium of the highest density (200/L) cultivated for the shortest time (24 h) provoked the strongest effect. <i>D.magna</i> spent medium negatively impacted the photosynthetic activity of <i>M. aeruginosa</i> PCC7806, as well as the dynamics of intracellular and extracellular cyanobacterial metabolites, while its mutant was unaffected. In the presence of <i>Daphnia</i> medium, microcystin does not appear to have a protective role for the strain. On the contrary, extracellular cyanopeptolin A increased in <i>M. aeruginosa</i> PCC7806 although the potential anti-grazing role of this compound would require further studies. |
| first_indexed | 2024-04-13T06:36:31Z |
| format | Article |
| id | doaj.art-6df66085e3b44caf94cc5e13bd8ea126 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T06:36:31Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-6df66085e3b44caf94cc5e13bd8ea1262022-12-22T02:57:53ZengMDPI AGToxins2072-66512019-07-0111742110.3390/toxins11070421toxins11070421<i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i>Gorenka Bojadzija Savic0Christine Edwards1Enora Briand2Linda Lawton3Claudia Wiegand4Myriam Bormans5Univ Rennes, CNRS, ECOBIO-UMR 6553, F-35000 Rennes, FranceSchool of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, UKIFREMER, Phycotoxins Laboratory, F-44311 Nantes, FranceSchool of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, UKUniv Rennes, CNRS, ECOBIO-UMR 6553, F-35000 Rennes, FranceUniv Rennes, CNRS, ECOBIO-UMR 6553, F-35000 Rennes, FranceWhile the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer <i>Daphnia</i> reduce cyanobacterial dominance until a certain density, which may be supported by <i>Daphnia</i> exudates, affecting the cyanobacterial physiological state and metabolites’ production. Therefore, we hypothesized that <i>D. magna</i> spent medium will impact the production of cyanobacterial bioactive metabolites and affect cyanobacterial photosynthetic activity in the nontoxic, but not the toxic strain. Microcystin (MC-LR and des-MC-LR) producing <i>M. aeruginosa</i> PCC7806 and its non-microcystin producing mutant were exposed to spent media of different <i>D. magna</i> densities and culture durations. <i>D. magna</i> spent medium of the highest density (200/L) cultivated for the shortest time (24 h) provoked the strongest effect. <i>D.magna</i> spent medium negatively impacted the photosynthetic activity of <i>M. aeruginosa</i> PCC7806, as well as the dynamics of intracellular and extracellular cyanobacterial metabolites, while its mutant was unaffected. In the presence of <i>Daphnia</i> medium, microcystin does not appear to have a protective role for the strain. On the contrary, extracellular cyanopeptolin A increased in <i>M. aeruginosa</i> PCC7806 although the potential anti-grazing role of this compound would require further studies.https://www.mdpi.com/2072-6651/11/7/421cyanobacteriasecondary metabolitesPCC7806toxicmutantinfochemicals |
| spellingShingle | Gorenka Bojadzija Savic Christine Edwards Enora Briand Linda Lawton Claudia Wiegand Myriam Bormans <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> Toxins cyanobacteria secondary metabolites PCC7806 toxic mutant infochemicals |
| title | <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> |
| title_full | <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> |
| title_fullStr | <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> |
| title_full_unstemmed | <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> |
| title_short | <i>Daphnia magna</i> Exudates Impact Physiological and Metabolic Changes in <i>Microcystis aeruginosa</i> |
| title_sort | i daphnia magna i exudates impact physiological and metabolic changes in i microcystis aeruginosa i |
| topic | cyanobacteria secondary metabolites PCC7806 toxic mutant infochemicals |
| url | https://www.mdpi.com/2072-6651/11/7/421 |
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