Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis
Syntrichia caninervis is a desiccation tolerant moss and is the dominant bryophyte found in biological soil crusts in the Gurbantunggut desert. In this study, we assessed the transcriptome profiles of S. caninervis gametophytes during the dehydration-rehydration (D-R) process (across 9 time points)...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1127541/full |
| _version_ | 1797897082463322112 |
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| author | Ruirui Yang Ruirui Yang Xiaoshuang Li Xiaoshuang Li Xiaoshuang Li Qilin Yang Qilin Yang Mingqi Zhao Mingqi Zhao Wenwan Bai Wenwan Bai Yuqing Liang Yuqing Liang Yuqing Liang Xiujin Liu Xiujin Liu Xiujin Liu Bei Gao Bei Gao Bei Gao Daoyuan Zhang Daoyuan Zhang Daoyuan Zhang |
| author_facet | Ruirui Yang Ruirui Yang Xiaoshuang Li Xiaoshuang Li Xiaoshuang Li Qilin Yang Qilin Yang Mingqi Zhao Mingqi Zhao Wenwan Bai Wenwan Bai Yuqing Liang Yuqing Liang Yuqing Liang Xiujin Liu Xiujin Liu Xiujin Liu Bei Gao Bei Gao Bei Gao Daoyuan Zhang Daoyuan Zhang Daoyuan Zhang |
| author_sort | Ruirui Yang |
| collection | DOAJ |
| description | Syntrichia caninervis is a desiccation tolerant moss and is the dominant bryophyte found in biological soil crusts in the Gurbantunggut desert. In this study, we assessed the transcriptome profiles of S. caninervis gametophytes during the dehydration-rehydration (D-R) process (across 9 time points) using Illumina sequencing. In total, 22489 transcripts were identified, including 5337 novel transcripts, that mapped to the reference genome. A total of 12548 transcripts exhibited significant alterations in the D-R samples compared with the control samples. The differentially expressed transcripts (DETs) possessed several enriched Gene Ontology terms, such as “water stress response”, “oxidation-reduction process”, “membrane metabolism”, “photosynthesis”, and “transcription factor activity”. Moreover, during early dehydration stress, the DETs were significantly enriched in stress-related pathways from the Kyoto Encyclopedia of Genes and Genomes, such as “phenylpropanoid biosynthesis”, “alpha-linolenic acid metabolism”, and “fructose and mannose metabolism”. Photosynthesis-related transcripts (e.g., ScPsa H, ScRubisco, and ScLhcb1) were inhibited during the dehydration treatment and significantly accumulated during the late rehydration period. Most transcripts from the late embryogenesis abundant proteins (LEA) and early light-inducible protein (ELIP) families strongly accumulated at the late dehydration stage. These pathways were positively correlated with the content changes of absolute water content and Fv/Fm values, alongside peroxidase and superoxide dismutase activities. Seven transcription factor families, including AP2-ERF, bHLH, G2-like, MYB, NAC, WRKY, and bZIP, were enriched in DETs during D-R treatment. This study is the first transcriptome analysis using the S. caninervis genome for gene annotation and multigroup D-R treatment points. Our results demonstrated the detailed dynamic changes in the transcriptome of S. caninervis during the D-R process. These results also improve understanding of desiccation tolerant plants’ adaptations to desiccation stress at the transcription level and provide promising gene resources for transgenic crop breeding. |
| first_indexed | 2024-04-10T07:52:05Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-10T07:52:05Z |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-6df6afeca60449339898180580a5fda42023-02-23T08:20:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11275411127541Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervisRuirui Yang0Ruirui Yang1Xiaoshuang Li2Xiaoshuang Li3Xiaoshuang Li4Qilin Yang5Qilin Yang6Mingqi Zhao7Mingqi Zhao8Wenwan Bai9Wenwan Bai10Yuqing Liang11Yuqing Liang12Yuqing Liang13Xiujin Liu14Xiujin Liu15Xiujin Liu16Bei Gao17Bei Gao18Bei Gao19Daoyuan Zhang20Daoyuan Zhang21Daoyuan Zhang22State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaXinjiang Key Lab of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaTurpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaXinjiang Key Lab of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaTurpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaXinjiang Key Lab of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaTurpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaXinjiang Key Lab of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaTurpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, ChinaState Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaXinjiang Key Lab of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, ChinaTurpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, ChinaSyntrichia caninervis is a desiccation tolerant moss and is the dominant bryophyte found in biological soil crusts in the Gurbantunggut desert. In this study, we assessed the transcriptome profiles of S. caninervis gametophytes during the dehydration-rehydration (D-R) process (across 9 time points) using Illumina sequencing. In total, 22489 transcripts were identified, including 5337 novel transcripts, that mapped to the reference genome. A total of 12548 transcripts exhibited significant alterations in the D-R samples compared with the control samples. The differentially expressed transcripts (DETs) possessed several enriched Gene Ontology terms, such as “water stress response”, “oxidation-reduction process”, “membrane metabolism”, “photosynthesis”, and “transcription factor activity”. Moreover, during early dehydration stress, the DETs were significantly enriched in stress-related pathways from the Kyoto Encyclopedia of Genes and Genomes, such as “phenylpropanoid biosynthesis”, “alpha-linolenic acid metabolism”, and “fructose and mannose metabolism”. Photosynthesis-related transcripts (e.g., ScPsa H, ScRubisco, and ScLhcb1) were inhibited during the dehydration treatment and significantly accumulated during the late rehydration period. Most transcripts from the late embryogenesis abundant proteins (LEA) and early light-inducible protein (ELIP) families strongly accumulated at the late dehydration stage. These pathways were positively correlated with the content changes of absolute water content and Fv/Fm values, alongside peroxidase and superoxide dismutase activities. Seven transcription factor families, including AP2-ERF, bHLH, G2-like, MYB, NAC, WRKY, and bZIP, were enriched in DETs during D-R treatment. This study is the first transcriptome analysis using the S. caninervis genome for gene annotation and multigroup D-R treatment points. Our results demonstrated the detailed dynamic changes in the transcriptome of S. caninervis during the D-R process. These results also improve understanding of desiccation tolerant plants’ adaptations to desiccation stress at the transcription level and provide promising gene resources for transgenic crop breeding.https://www.frontiersin.org/articles/10.3389/fpls.2023.1127541/fullSyntrichia caninervisdesiccation tolerancephenylpropanoid biosynthesisalpha-linolenic acidphotosynthesistranscription factor |
| spellingShingle | Ruirui Yang Ruirui Yang Xiaoshuang Li Xiaoshuang Li Xiaoshuang Li Qilin Yang Qilin Yang Mingqi Zhao Mingqi Zhao Wenwan Bai Wenwan Bai Yuqing Liang Yuqing Liang Yuqing Liang Xiujin Liu Xiujin Liu Xiujin Liu Bei Gao Bei Gao Bei Gao Daoyuan Zhang Daoyuan Zhang Daoyuan Zhang Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis Frontiers in Plant Science Syntrichia caninervis desiccation tolerance phenylpropanoid biosynthesis alpha-linolenic acid photosynthesis transcription factor |
| title | Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis |
| title_full | Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis |
| title_fullStr | Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis |
| title_full_unstemmed | Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis |
| title_short | Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis |
| title_sort | transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss syntrichia caninervis |
| topic | Syntrichia caninervis desiccation tolerance phenylpropanoid biosynthesis alpha-linolenic acid photosynthesis transcription factor |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1127541/full |
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