Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition
HIGHLIGHTSBiomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps a...
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Frontiers Media S.A.
2017-05-01
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| Շարք: | Frontiers in Plant Science |
| Խորագրեր: | |
| Առցանց հասանելիություն: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00740/full |
| _version_ | 1828530934983426048 |
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| author | Nassim Belmokhtar Stéphanie Arnoult Brigitte Chabbert Jean-Paul Charpentier Maryse Brancourt-Hulmel |
| author_facet | Nassim Belmokhtar Stéphanie Arnoult Brigitte Chabbert Jean-Paul Charpentier Maryse Brancourt-Hulmel |
| author_sort | Nassim Belmokhtar |
| collection | DOAJ |
| description | HIGHLIGHTSBiomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale.The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries. The saccharification potential and year variability of five genotypes belonging to Miscanthus × giganteus and Miscanthus sinensis were explored using a miniaturized and semi-automated method, allowing the application of a hot water treatment followed by an enzymatic hydrolysis. The studied genotypes highlighted distinct cellulose conversion yields due to their distinct cell wall compositions. An inter-year comparison revealed significant variations in the biomass productivity and cell wall compositions. Compared to the recalcitrant genotypes, more digestible genotypes contained higher amounts of hemicellulosic carbohydrates and lower amounts of cellulose and lignin. In contrast to hemicellulosic carbohydrates, the relationships analysis between the biomass traits and cellulose conversion clearly showed the same negative effect of cellulose and lignin on cellulose digestion. The miniaturized and semi-automated method we developed was usable at the laboratory scale and was reliable for mimicking the saccharification at the pilot scale using a steam explosion pretreatment and enzymatic hydrolysis. Therefore, this miniaturized method will allow the reliable screening of many genotypes for saccharification potential. These findings provide valuable information and tools for breeders to create genotypes combining high yield, suitable biomass composition, and high saccharification yields. |
| first_indexed | 2024-12-11T22:30:16Z |
| format | Article |
| id | doaj.art-6df9a3cd566f4b51aeb0f57a4681b511 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T22:30:16Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6df9a3cd566f4b51aeb0f57a4681b5112022-12-22T00:48:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00740248965Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass CompositionNassim Belmokhtar0Stéphanie Arnoult1Brigitte Chabbert2Jean-Paul Charpentier3Maryse Brancourt-Hulmel4AGPF, Institut National de la Recherche AgronomiqueOrléans, FranceGCIE-Picardie, Institut National de la Recherche AgronomiqueEstrées-Mons, FranceFARE Laboratory, Institut National de la Recherche Agronomique, Université de Reims Champagne-ArdenneReims, FranceAGPF, Institut National de la Recherche AgronomiqueOrléans, FranceAgroImpact Unit, Institut National de la Recherche AgronomiqueEstrées-Mons, FranceHIGHLIGHTSBiomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale.The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries. The saccharification potential and year variability of five genotypes belonging to Miscanthus × giganteus and Miscanthus sinensis were explored using a miniaturized and semi-automated method, allowing the application of a hot water treatment followed by an enzymatic hydrolysis. The studied genotypes highlighted distinct cellulose conversion yields due to their distinct cell wall compositions. An inter-year comparison revealed significant variations in the biomass productivity and cell wall compositions. Compared to the recalcitrant genotypes, more digestible genotypes contained higher amounts of hemicellulosic carbohydrates and lower amounts of cellulose and lignin. In contrast to hemicellulosic carbohydrates, the relationships analysis between the biomass traits and cellulose conversion clearly showed the same negative effect of cellulose and lignin on cellulose digestion. The miniaturized and semi-automated method we developed was usable at the laboratory scale and was reliable for mimicking the saccharification at the pilot scale using a steam explosion pretreatment and enzymatic hydrolysis. Therefore, this miniaturized method will allow the reliable screening of many genotypes for saccharification potential. These findings provide valuable information and tools for breeders to create genotypes combining high yield, suitable biomass composition, and high saccharification yields.http://journal.frontiersin.org/article/10.3389/fpls.2017.00740/fullMiscanthushigh-throughput pretreatment and saccharificationpilot-scale pretreatment and saccharificationhemicellulosescelluloselignin |
| spellingShingle | Nassim Belmokhtar Stéphanie Arnoult Brigitte Chabbert Jean-Paul Charpentier Maryse Brancourt-Hulmel Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition Frontiers in Plant Science Miscanthus high-throughput pretreatment and saccharification pilot-scale pretreatment and saccharification hemicelluloses cellulose lignin |
| title | Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition |
| title_full | Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition |
| title_fullStr | Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition |
| title_full_unstemmed | Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition |
| title_short | Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition |
| title_sort | saccharification performances of miscanthus at the pilot and miniaturized assay scales genotype and year variabilities according to the biomass composition |
| topic | Miscanthus high-throughput pretreatment and saccharification pilot-scale pretreatment and saccharification hemicelluloses cellulose lignin |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00740/full |
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