Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells
Abstract Background Cervical cancer is the fourth most common cancer affecting women worldwide. Paclitaxel/Carboplatin is one of the most commonly prescribed regimens in cervical cancer treatment. Although chemotherapeutic drugs are very effective, severe side effects and development of drug resista...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-019-0868-0 |
| _version_ | 1819203545745850368 |
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| author | Nora M. Aborehab Nada Osama |
| author_facet | Nora M. Aborehab Nada Osama |
| author_sort | Nora M. Aborehab |
| collection | DOAJ |
| description | Abstract Background Cervical cancer is the fourth most common cancer affecting women worldwide. Paclitaxel/Carboplatin is one of the most commonly prescribed regimens in cervical cancer treatment. Although chemotherapeutic drugs are very effective, severe side effects and development of drug resistance limits the use of these drugs. The use of natural products with anticancer activity may help to partially overcome these issues. Methods In the present study, we investigated the ability of Gallic acid, to potentiate the anti-cancer effects of Paclitaxel, Carboplatin and Paclitaxel/Carboplatin combination in human HeLa cells by performing MTT assay, cell cycle analysis and RT-PCR assay and Western blotting for some apoptotic markers. Results Our results revealed that the highest cytotoxic effect, the highest induction of apoptosis and significant elevation in P53 and Caspase 3 levels was seen in Paclitaxel/Gallic acid combination. Conclusion These results indicate that Gallic acid potentiates Paclitaxel effect and that Paclitaxel/Gallic acid combination could represent a promising alternative with lower side effects-for Paclitaxel/Carboplatin combination in treatment of cervical cancer treatment. |
| first_indexed | 2024-12-23T04:21:37Z |
| format | Article |
| id | doaj.art-6dfc45c14ae045539627d215ac126446 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-23T04:21:37Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-6dfc45c14ae045539627d215ac1264462022-12-21T18:00:14ZengBMCCancer Cell International1475-28672019-06-0119111310.1186/s12935-019-0868-0Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cellsNora M. Aborehab0Nada Osama1Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA University)Department of Biochemistry, Faculty of Pharmacy, Menoufia UniversityAbstract Background Cervical cancer is the fourth most common cancer affecting women worldwide. Paclitaxel/Carboplatin is one of the most commonly prescribed regimens in cervical cancer treatment. Although chemotherapeutic drugs are very effective, severe side effects and development of drug resistance limits the use of these drugs. The use of natural products with anticancer activity may help to partially overcome these issues. Methods In the present study, we investigated the ability of Gallic acid, to potentiate the anti-cancer effects of Paclitaxel, Carboplatin and Paclitaxel/Carboplatin combination in human HeLa cells by performing MTT assay, cell cycle analysis and RT-PCR assay and Western blotting for some apoptotic markers. Results Our results revealed that the highest cytotoxic effect, the highest induction of apoptosis and significant elevation in P53 and Caspase 3 levels was seen in Paclitaxel/Gallic acid combination. Conclusion These results indicate that Gallic acid potentiates Paclitaxel effect and that Paclitaxel/Gallic acid combination could represent a promising alternative with lower side effects-for Paclitaxel/Carboplatin combination in treatment of cervical cancer treatment.http://link.springer.com/article/10.1186/s12935-019-0868-0HeLa cellsGallic acidPaclitaxelP53Caspase 3 |
| spellingShingle | Nora M. Aborehab Nada Osama Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells Cancer Cell International HeLa cells Gallic acid Paclitaxel P53 Caspase 3 |
| title | Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells |
| title_full | Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells |
| title_fullStr | Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells |
| title_full_unstemmed | Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells |
| title_short | Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells |
| title_sort | effect of gallic acid in potentiating chemotherapeutic effect of paclitaxel in hela cervical cancer cells |
| topic | HeLa cells Gallic acid Paclitaxel P53 Caspase 3 |
| url | http://link.springer.com/article/10.1186/s12935-019-0868-0 |
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