Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds

The Khurasani-ajvayan (Hyoscyamus niger L) is an important medicinal plant which is having high medicinal value. The germination test was conducted in the laboratory at three different temperatures (20, 25 and 30°C) and substrata (BP, TP and S). The results revealed that species having high percent...

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Main Authors: SHER SINGH VERMA, P K VERMA, URMIL VERMA
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2014-11-01
Series:The Indian Journal of Agricultural Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAgS/article/view/44643
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author SHER SINGH VERMA
P K VERMA
URMIL VERMA
author_facet SHER SINGH VERMA
P K VERMA
URMIL VERMA
author_sort SHER SINGH VERMA
collection DOAJ
description The Khurasani-ajvayan (Hyoscyamus niger L) is an important medicinal plant which is having high medicinal value. The germination test was conducted in the laboratory at three different temperatures (20, 25 and 30°C) and substrata (BP, TP and S). The results revealed that species having high percentage of hard seeds ranged from 58.0 -74.0% at 20°C followed by 25°C and 30°C which ranged from 42.0- 55.0% and 44.0 to 47.0%, respectively in all the substrata after 15 days of planting. To overcome this problem of low germination, the seeds were further subjected to various treatments, viz. seed soaking in water (24 and 48 hr), GA3 (200 and 500ppm), KNO3 (0.2%) and conc. sulphuric acid (H2SO4) 15 seconds to enhance seed germination and tested for seed germination after seed treatment. Among these treatments, GA3 (200 ppm) recorded the maximum normal seedlings (73.0%) in BP substrata followed by TP (68.0%) at 30°C after 12 days. Hard seeds were also reduced after soaking the seeds in water for 48 hr but it resulted in a subsequent increase in the dead seeds. KNO3 (0.2%) and GA3 (500 ppm) seed treatments enhanced the germination up to 50% as compared to control after 13 and 14 days, respectively irrespective of substrata and temperatures. Therefore, it is suggested that the seeds of Khurasani-ajvayan should be tested for germination in BP/TP substrata at 30°C after soaking the seeds in GA3 (200 ppm) solution for 24hours. The final count for germination should be taken after 12 days.
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spelling doaj.art-6dff1a1de55347dab127fd4892f27db12023-08-25T04:58:05ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192014-11-01841110.56093/ijas.v84i11.44643Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seedsSHER SINGH VERMA0P K VERMA1URMIL VERMA2CCS Haryana Agricultural University, Hisar, Haryana 125 004CCS Haryana Agricultural University, Hisar, Haryana 125 004CCS Haryana Agricultural University, Hisar, Haryana 125 004 The Khurasani-ajvayan (Hyoscyamus niger L) is an important medicinal plant which is having high medicinal value. The germination test was conducted in the laboratory at three different temperatures (20, 25 and 30°C) and substrata (BP, TP and S). The results revealed that species having high percentage of hard seeds ranged from 58.0 -74.0% at 20°C followed by 25°C and 30°C which ranged from 42.0- 55.0% and 44.0 to 47.0%, respectively in all the substrata after 15 days of planting. To overcome this problem of low germination, the seeds were further subjected to various treatments, viz. seed soaking in water (24 and 48 hr), GA3 (200 and 500ppm), KNO3 (0.2%) and conc. sulphuric acid (H2SO4) 15 seconds to enhance seed germination and tested for seed germination after seed treatment. Among these treatments, GA3 (200 ppm) recorded the maximum normal seedlings (73.0%) in BP substrata followed by TP (68.0%) at 30°C after 12 days. Hard seeds were also reduced after soaking the seeds in water for 48 hr but it resulted in a subsequent increase in the dead seeds. KNO3 (0.2%) and GA3 (500 ppm) seed treatments enhanced the germination up to 50% as compared to control after 13 and 14 days, respectively irrespective of substrata and temperatures. Therefore, it is suggested that the seeds of Khurasani-ajvayan should be tested for germination in BP/TP substrata at 30°C after soaking the seeds in GA3 (200 ppm) solution for 24hours. The final count for germination should be taken after 12 days. https://epubs.icar.org.in/index.php/IJAgS/article/view/44643DormancyGerminationHard seedsISTAKhurasani-ajvayanNormal seedlings
spellingShingle SHER SINGH VERMA
P K VERMA
URMIL VERMA
Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
The Indian Journal of Agricultural Sciences
Dormancy
Germination
Hard seeds
ISTA
Khurasani-ajvayan
Normal seedlings
title Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
title_full Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
title_fullStr Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
title_full_unstemmed Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
title_short Standardization of seed germination technique in Khurasani-ajavayan (Hyoscyamus niger) seeds
title_sort standardization of seed germination technique in khurasani ajavayan hyoscyamus niger seeds
topic Dormancy
Germination
Hard seeds
ISTA
Khurasani-ajvayan
Normal seedlings
url https://epubs.icar.org.in/index.php/IJAgS/article/view/44643
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AT pkverma standardizationofseedgerminationtechniqueinkhurasaniajavayanhyoscyamusnigerseeds
AT urmilverma standardizationofseedgerminationtechniqueinkhurasaniajavayanhyoscyamusnigerseeds