Generation of Gellan Gum-Based Adipose-Like Microtissues
Adipose tissue is involved in many physiological processes. Therefore, the need for adipose tissue-like analogues either for soft tissue reconstruction or as in vitro testing platforms is undeniable. In this work, we explored the natural features of gellan gum (GG) to recreate injectable stable adip...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-06-01
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| Series: | Bioengineering |
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| Online Access: | http://www.mdpi.com/2306-5354/5/3/52 |
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| author | Manuela E. L. Lago Lucília P. da Silva Catarina Henriques Andreia F. Carvalho Rui L. Reis Alexandra P. Marques |
| author_facet | Manuela E. L. Lago Lucília P. da Silva Catarina Henriques Andreia F. Carvalho Rui L. Reis Alexandra P. Marques |
| author_sort | Manuela E. L. Lago |
| collection | DOAJ |
| description | Adipose tissue is involved in many physiological processes. Therefore, the need for adipose tissue-like analogues either for soft tissue reconstruction or as in vitro testing platforms is undeniable. In this work, we explored the natural features of gellan gum (GG) to recreate injectable stable adipose-like microtissues. GG hydrogel particles with different percentages of polymer (0.5%, 0.75%, 1.25%) were developed and the effect of obtained mechanical properties over the ability of hASCs to differentiate towards the adipogenic lineage was evaluated based on the expression of the early (PPARγ) and late (FABP4) adipogenic markers, and on lipids formation and accumulation. Constructs were cultured in adipogenic induction medium up to 21 days or for six days in induction plus nine days in maintenance media. Overall, no significant differences were observed in terms of hASCs adipogenic differentiation within the range of Young’s moduli between 2.7 and 12.9 kPa. The long-term (up to six weeks) stability of the developed constructs supported its application in soft tissue reconstruction. Moreover, their ability to function as adipose-like microtissue models for drug screening was demonstrated by confirming its sensitivity to TNFα and ROCK inhibitor, respectively involved in the repression and induction of the adipogenic differentiation. |
| first_indexed | 2024-03-12T09:39:10Z |
| format | Article |
| id | doaj.art-6e02810885a245e09b5ed2763d598d84 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T09:39:10Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-6e02810885a245e09b5ed2763d598d842023-09-02T13:23:32ZengMDPI AGBioengineering2306-53542018-06-01535210.3390/bioengineering5030052bioengineering5030052Generation of Gellan Gum-Based Adipose-Like MicrotissuesManuela E. L. Lago0Lucília P. da Silva1Catarina Henriques2Andreia F. Carvalho3Rui L. Reis4Alexandra P. Marques53B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Avepark, Barco, 4805-017 Guimarães, PortugalAdipose tissue is involved in many physiological processes. Therefore, the need for adipose tissue-like analogues either for soft tissue reconstruction or as in vitro testing platforms is undeniable. In this work, we explored the natural features of gellan gum (GG) to recreate injectable stable adipose-like microtissues. GG hydrogel particles with different percentages of polymer (0.5%, 0.75%, 1.25%) were developed and the effect of obtained mechanical properties over the ability of hASCs to differentiate towards the adipogenic lineage was evaluated based on the expression of the early (PPARγ) and late (FABP4) adipogenic markers, and on lipids formation and accumulation. Constructs were cultured in adipogenic induction medium up to 21 days or for six days in induction plus nine days in maintenance media. Overall, no significant differences were observed in terms of hASCs adipogenic differentiation within the range of Young’s moduli between 2.7 and 12.9 kPa. The long-term (up to six weeks) stability of the developed constructs supported its application in soft tissue reconstruction. Moreover, their ability to function as adipose-like microtissue models for drug screening was demonstrated by confirming its sensitivity to TNFα and ROCK inhibitor, respectively involved in the repression and induction of the adipogenic differentiation.http://www.mdpi.com/2306-5354/5/3/52adipose tissue engineeringadipogenic differentiationadipose-like microtissuesgellan gumhydrogels |
| spellingShingle | Manuela E. L. Lago Lucília P. da Silva Catarina Henriques Andreia F. Carvalho Rui L. Reis Alexandra P. Marques Generation of Gellan Gum-Based Adipose-Like Microtissues Bioengineering adipose tissue engineering adipogenic differentiation adipose-like microtissues gellan gum hydrogels |
| title | Generation of Gellan Gum-Based Adipose-Like Microtissues |
| title_full | Generation of Gellan Gum-Based Adipose-Like Microtissues |
| title_fullStr | Generation of Gellan Gum-Based Adipose-Like Microtissues |
| title_full_unstemmed | Generation of Gellan Gum-Based Adipose-Like Microtissues |
| title_short | Generation of Gellan Gum-Based Adipose-Like Microtissues |
| title_sort | generation of gellan gum based adipose like microtissues |
| topic | adipose tissue engineering adipogenic differentiation adipose-like microtissues gellan gum hydrogels |
| url | http://www.mdpi.com/2306-5354/5/3/52 |
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