Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis
Metabolomic analysis is an emerging new diagnostic tool, which holds great potential for improving the understanding of osteoarthritis (OA)-caused metabolomic shifts associated with systemic inflammation and oxidative stress. The main aim of the study was to map the changes of amino acid, biogenic a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/8/323 |
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| author | Kaspar Tootsi Kadri Vilba Aare Märtson Jaak Kals Kaido Paapstel Mihkel Zilmer |
| author_facet | Kaspar Tootsi Kadri Vilba Aare Märtson Jaak Kals Kaido Paapstel Mihkel Zilmer |
| author_sort | Kaspar Tootsi |
| collection | DOAJ |
| description | Metabolomic analysis is an emerging new diagnostic tool, which holds great potential for improving the understanding of osteoarthritis (OA)-caused metabolomic shifts associated with systemic inflammation and oxidative stress. The main aim of the study was to map the changes of amino acid, biogenic amine and complex lipid profiles in severe OA, where the shifts should be more eminent compared with early stages. The fasting serum of 70 knee and hip OA patients and 82 controls was assessed via a targeted approach using the AbsoluteIDQ™ p180 kit. Changes in the serum levels of amino acids, sphingomyelins, phoshatidylcholines and lysophosphatidylcholines of the OA patients compared with controls suggest systemic inflammation in severe OA patients. Furthermore, the decreased spermine to spermidine ratio indicates excessive oxidative stress to be associated with OA. Serum arginine level was positively correlated with radiographic severity of OA, potentially linking inflammation through NO synthesis to OA. Further, the level of glycine was negatively associated with the severity of OA, which might refer to glycine deficiency in severe OA. The current study demonstrates significant changes in the amino acid, biogenic amine and low-molecular weight lipid profiles of severe OA and provides new insights into the complex interplay between chronic inflammation, oxidative stress and OA. |
| first_indexed | 2024-03-10T17:46:55Z |
| format | Article |
| id | doaj.art-6e068402e6784a03afaaf64bf5546704 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T17:46:55Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6e068402e6784a03afaaf64bf55467042023-11-20T09:30:14ZengMDPI AGMetabolites2218-19892020-08-0110832310.3390/metabo10080323Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe OsteoarthritisKaspar Tootsi0Kadri Vilba1Aare Märtson2Jaak Kals3Kaido Paapstel4Mihkel Zilmer5Department of Traumatology and Orthopaedics, University of Tartu, 50090 Tartu, EstoniaDepartment of Traumatology and Orthopaedics, University of Tartu, 50090 Tartu, EstoniaDepartment of Traumatology and Orthopaedics, University of Tartu, 50090 Tartu, EstoniaEndothelial Centre, University of Tartu, 50090 Tartu, EstoniaEndothelial Centre, University of Tartu, 50090 Tartu, EstoniaEndothelial Centre, University of Tartu, 50090 Tartu, EstoniaMetabolomic analysis is an emerging new diagnostic tool, which holds great potential for improving the understanding of osteoarthritis (OA)-caused metabolomic shifts associated with systemic inflammation and oxidative stress. The main aim of the study was to map the changes of amino acid, biogenic amine and complex lipid profiles in severe OA, where the shifts should be more eminent compared with early stages. The fasting serum of 70 knee and hip OA patients and 82 controls was assessed via a targeted approach using the AbsoluteIDQ™ p180 kit. Changes in the serum levels of amino acids, sphingomyelins, phoshatidylcholines and lysophosphatidylcholines of the OA patients compared with controls suggest systemic inflammation in severe OA patients. Furthermore, the decreased spermine to spermidine ratio indicates excessive oxidative stress to be associated with OA. Serum arginine level was positively correlated with radiographic severity of OA, potentially linking inflammation through NO synthesis to OA. Further, the level of glycine was negatively associated with the severity of OA, which might refer to glycine deficiency in severe OA. The current study demonstrates significant changes in the amino acid, biogenic amine and low-molecular weight lipid profiles of severe OA and provides new insights into the complex interplay between chronic inflammation, oxidative stress and OA.https://www.mdpi.com/2218-1989/10/8/323osteoarthritismetabolitesmetabolomicslipidomicsamino acidslipids |
| spellingShingle | Kaspar Tootsi Kadri Vilba Aare Märtson Jaak Kals Kaido Paapstel Mihkel Zilmer Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis Metabolites osteoarthritis metabolites metabolomics lipidomics amino acids lipids |
| title | Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis |
| title_full | Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis |
| title_fullStr | Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis |
| title_full_unstemmed | Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis |
| title_short | Metabolomic Signature of Amino Acids, Biogenic Amines and Lipids in Blood Serum of Patients with Severe Osteoarthritis |
| title_sort | metabolomic signature of amino acids biogenic amines and lipids in blood serum of patients with severe osteoarthritis |
| topic | osteoarthritis metabolites metabolomics lipidomics amino acids lipids |
| url | https://www.mdpi.com/2218-1989/10/8/323 |
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