Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function
Kylie BR Belchamber, Catherine MR Thomas, Amy E Dunne, Peter J Barnes, Louise E Donnelly Airway Disease Section, National Heart and Lung Institute, Dovehouse Street, Imperial College London, London, UK Background: Inhaled corticosteroid use is associated with increased rates of pneumonia in COPD p...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-09-01
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| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/comparison-of-fluticasone-propionate-and-budesonide-on-copd-macrophage-peer-reviewed-article-COPD |
| _version_ | 1818964605611802624 |
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| author | Belchamber KBR Thomas CMR Dunne AE Barnes PJ Donnelly LE |
| author_facet | Belchamber KBR Thomas CMR Dunne AE Barnes PJ Donnelly LE |
| author_sort | Belchamber KBR |
| collection | DOAJ |
| description | Kylie BR Belchamber, Catherine MR Thomas, Amy E Dunne, Peter J Barnes, Louise E Donnelly Airway Disease Section, National Heart and Lung Institute, Dovehouse Street, Imperial College London, London, UK Background: Inhaled corticosteroid use is associated with increased rates of pneumonia in COPD patients. The underlying mechanism is unknown, although recent data suggest that pneumonia is more frequent in patients treated with fluticasone propionate (FP) than budesonide. Macrophages and neutrophils from COPD patients are deficient in clearing bacteria, and this might explain increased bacterial colonization in COPD. Inhaled corticosteroid may further suppress this response; therefore, we examined the effect of FP and budesonide on phagocytosis of common respiratory pathogens by monocyte-derived macrophages (MDMs) and neutrophils.Methods: MDMs from COPD patients (n=20–24) were preincubated with FP or budesonide for 1 or 18 hours, after which phagocytosis of fluorescently labeled inert beads or heat-killed Haemophilus influenzae/Streptococcus pneumoniae were measured fluorimetrically after 1 or 4 hours. Additionally, CXCL8, IL6, and TNFα concentrations in supernatants by ELISA, MDM-scavenger-receptor expression by flow cytometry, and MDM ability to kill bacteria were measured. Neutrophils from COPD patients (n=8) were preincubated with corticosteroids for 1 hour and bacteria phagocytosis measured by flow cytometry.Results: After 1 hour’s preincubation, neither corticosteroid altered MDM phagocytosis of beads or H. influenzae; however, budesonide (10-7 M) increased S. pneumoniae phagocytosis by 23% (P<0.05). After 18 hours’ preincubation, neither corticosteroid altered MDM phagocytosis of any prey, although H. influenzae phagocytosis by budesonide was significantly greater compared to FP at 10-6 and 10-5 M (P<0.05). The 1-hour preincubation with either corticosteroid inhibited bacteria-induced CXCL8 release (at 10-7 and 10-5 M, P<0.05); however, this effect was lost at 18-hour preincubation. There was no change in receptor expression, bacterial killing, or neutrophil phagocytosis by either corticosteroid.Conclusion: These data suggest that dissolved FP and budesonide do not have an overall effect on MDM or neutrophil phagocytosis of bacteria. Keywords: COPD, macrophage, neutrophil, fluticasone propionate, budesonide |
| first_indexed | 2024-12-20T13:03:46Z |
| format | Article |
| id | doaj.art-6e07f8f5209941acaf8269fa031d08ac |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-12-20T13:03:46Z |
| publishDate | 2018-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-6e07f8f5209941acaf8269fa031d08ac2022-12-21T19:39:50ZengDove Medical PressInternational Journal of COPD1178-20052018-09-01Volume 132883289740655Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil functionBelchamber KBRThomas CMRDunne AEBarnes PJDonnelly LEKylie BR Belchamber, Catherine MR Thomas, Amy E Dunne, Peter J Barnes, Louise E Donnelly Airway Disease Section, National Heart and Lung Institute, Dovehouse Street, Imperial College London, London, UK Background: Inhaled corticosteroid use is associated with increased rates of pneumonia in COPD patients. The underlying mechanism is unknown, although recent data suggest that pneumonia is more frequent in patients treated with fluticasone propionate (FP) than budesonide. Macrophages and neutrophils from COPD patients are deficient in clearing bacteria, and this might explain increased bacterial colonization in COPD. Inhaled corticosteroid may further suppress this response; therefore, we examined the effect of FP and budesonide on phagocytosis of common respiratory pathogens by monocyte-derived macrophages (MDMs) and neutrophils.Methods: MDMs from COPD patients (n=20–24) were preincubated with FP or budesonide for 1 or 18 hours, after which phagocytosis of fluorescently labeled inert beads or heat-killed Haemophilus influenzae/Streptococcus pneumoniae were measured fluorimetrically after 1 or 4 hours. Additionally, CXCL8, IL6, and TNFα concentrations in supernatants by ELISA, MDM-scavenger-receptor expression by flow cytometry, and MDM ability to kill bacteria were measured. Neutrophils from COPD patients (n=8) were preincubated with corticosteroids for 1 hour and bacteria phagocytosis measured by flow cytometry.Results: After 1 hour’s preincubation, neither corticosteroid altered MDM phagocytosis of beads or H. influenzae; however, budesonide (10-7 M) increased S. pneumoniae phagocytosis by 23% (P<0.05). After 18 hours’ preincubation, neither corticosteroid altered MDM phagocytosis of any prey, although H. influenzae phagocytosis by budesonide was significantly greater compared to FP at 10-6 and 10-5 M (P<0.05). The 1-hour preincubation with either corticosteroid inhibited bacteria-induced CXCL8 release (at 10-7 and 10-5 M, P<0.05); however, this effect was lost at 18-hour preincubation. There was no change in receptor expression, bacterial killing, or neutrophil phagocytosis by either corticosteroid.Conclusion: These data suggest that dissolved FP and budesonide do not have an overall effect on MDM or neutrophil phagocytosis of bacteria. Keywords: COPD, macrophage, neutrophil, fluticasone propionate, budesonidehttps://www.dovepress.com/comparison-of-fluticasone-propionate-and-budesonide-on-copd-macrophage-peer-reviewed-article-COPDCOPDmacrophageneutrophilfluticasone propionatebudesonide |
| spellingShingle | Belchamber KBR Thomas CMR Dunne AE Barnes PJ Donnelly LE Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function International Journal of COPD COPD macrophage neutrophil fluticasone propionate budesonide |
| title | Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function |
| title_full | Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function |
| title_fullStr | Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function |
| title_full_unstemmed | Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function |
| title_short | Comparison of fluticasone propionate and budesonide on COPD macrophage and neutrophil function |
| title_sort | comparison of fluticasone propionate and budesonide on copd macrophage and neutrophil function |
| topic | COPD macrophage neutrophil fluticasone propionate budesonide |
| url | https://www.dovepress.com/comparison-of-fluticasone-propionate-and-budesonide-on-copd-macrophage-peer-reviewed-article-COPD |
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