Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo
Engineered heart tissues (EHTs) that are fabricated using human induced pluripotent stem cells (hiPSCs) have been considered as potential cardiac tissue substitutes in case of heart failure. In the present study, we have created hiPSC-derived cardiac organoids (hiPSC-COs) comprised of hiPSC-derived...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2021.806215/full |
| _version_ | 1798015578567344128 |
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| author | Yujiro Kawai Shugo Tohyama Kenichi Arai Tadashi Tamura Yusuke Soma Keiichi Fukuda Hideyuki Shimizu Koichi Nakayama Eiji Kobayashi Eiji Kobayashi |
| author_facet | Yujiro Kawai Shugo Tohyama Kenichi Arai Tadashi Tamura Yusuke Soma Keiichi Fukuda Hideyuki Shimizu Koichi Nakayama Eiji Kobayashi Eiji Kobayashi |
| author_sort | Yujiro Kawai |
| collection | DOAJ |
| description | Engineered heart tissues (EHTs) that are fabricated using human induced pluripotent stem cells (hiPSCs) have been considered as potential cardiac tissue substitutes in case of heart failure. In the present study, we have created hiPSC-derived cardiac organoids (hiPSC-COs) comprised of hiPSC-derived cardiomyocytes, human umbilical vein endothelial cells, and human fibroblasts. To produce a beating conduit for patients suffering from congenital heart diseases, we constructed scaffold-free tubular EHTs (T-EHTs) using hiPSC-COs and bio-3D printing with needle arrays. The bio-3D printed T-EHTs were cut open and transplanted around the abdominal aorta as well as the inferior vena cava (IVC) of NOG mice. The transplanted T-EHTs were covered with the omentum, and the abdomen was closed after completion of the procedure. Additionally, to compare the functionality of hiPSC-COs with that of T-EHTs, we transplanted the former around the aorta and IVC as well as injecting them into the subcutaneous tissue on the back of the mice. After 1 m of the transplantation procedures, we observed the beating of the T-EHTs in the mice. In histological analysis, the T-EHTs showed clear striation of the myocardium and vascularization compared to hiPSC-COs transplanted around the aorta or in subcutaneous tissue. Based on these results, bio-3D-printed T-EHTs exhibited a better maturation in vivo as compared to the hiPSC-COs. Therefore, these beating T-EHTs may form conduits for congenital heart disease patients, and T-EHT transplantation can form a treatment option in such cases. |
| first_indexed | 2024-04-11T15:36:52Z |
| format | Article |
| id | doaj.art-6e0a773e5000476f9a2ce8bf3197f5e8 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-11T15:36:52Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-6e0a773e5000476f9a2ce8bf3197f5e82022-12-22T04:15:56ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-01-01810.3389/fcvm.2021.806215806215Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivoYujiro Kawai0Shugo Tohyama1Kenichi Arai2Tadashi Tamura3Yusuke Soma4Keiichi Fukuda5Hideyuki Shimizu6Koichi Nakayama7Eiji Kobayashi8Eiji Kobayashi9Department of Cardiovascular Surgery, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanDepartment of Regenerative Medicine and Biomedical Engineering, Saga University, Saga, JapanDepartment of Regenerative Medicine and Biomedical Engineering, Saga University, Saga, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiovascular Surgery, Keio University School of Medicine, Tokyo, JapanDepartment of Regenerative Medicine and Biomedical Engineering, Saga University, Saga, JapanDepartment of Organ Fabrication, Keio University School of Medicine, Tokyo, JapanDepartment of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo, JapanEngineered heart tissues (EHTs) that are fabricated using human induced pluripotent stem cells (hiPSCs) have been considered as potential cardiac tissue substitutes in case of heart failure. In the present study, we have created hiPSC-derived cardiac organoids (hiPSC-COs) comprised of hiPSC-derived cardiomyocytes, human umbilical vein endothelial cells, and human fibroblasts. To produce a beating conduit for patients suffering from congenital heart diseases, we constructed scaffold-free tubular EHTs (T-EHTs) using hiPSC-COs and bio-3D printing with needle arrays. The bio-3D printed T-EHTs were cut open and transplanted around the abdominal aorta as well as the inferior vena cava (IVC) of NOG mice. The transplanted T-EHTs were covered with the omentum, and the abdomen was closed after completion of the procedure. Additionally, to compare the functionality of hiPSC-COs with that of T-EHTs, we transplanted the former around the aorta and IVC as well as injecting them into the subcutaneous tissue on the back of the mice. After 1 m of the transplantation procedures, we observed the beating of the T-EHTs in the mice. In histological analysis, the T-EHTs showed clear striation of the myocardium and vascularization compared to hiPSC-COs transplanted around the aorta or in subcutaneous tissue. Based on these results, bio-3D-printed T-EHTs exhibited a better maturation in vivo as compared to the hiPSC-COs. Therefore, these beating T-EHTs may form conduits for congenital heart disease patients, and T-EHT transplantation can form a treatment option in such cases.https://www.frontiersin.org/articles/10.3389/fcvm.2021.806215/fullhuman-induced pluripotent stem cellengineered heart tissuebio-3D bioprintingtubular tissuecardiomyocyte |
| spellingShingle | Yujiro Kawai Shugo Tohyama Kenichi Arai Tadashi Tamura Yusuke Soma Keiichi Fukuda Hideyuki Shimizu Koichi Nakayama Eiji Kobayashi Eiji Kobayashi Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo Frontiers in Cardiovascular Medicine human-induced pluripotent stem cell engineered heart tissue bio-3D bioprinting tubular tissue cardiomyocyte |
| title | Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo |
| title_full | Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo |
| title_fullStr | Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo |
| title_full_unstemmed | Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo |
| title_short | Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo |
| title_sort | scaffold free tubular engineered heart tissue from human induced pluripotent stem cells using bio 3d printing technology in vivo |
| topic | human-induced pluripotent stem cell engineered heart tissue bio-3D bioprinting tubular tissue cardiomyocyte |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2021.806215/full |
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