Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance.
The present investigation was undertaken to test whether exercise training (ET) associated with AMPK/PPAR agonists (EM) would improve skeletal muscle function in mdx mice. These drugs have the potential to improve oxidative metabolism. This is of particular interest because oxidative muscle fibers a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3448675?pdf=render |
| _version_ | 1818939939491938304 |
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| author | Carlos R Bueno Júnior Lucas C Pantaleão Vanessa A Voltarelli Luiz Henrique M Bozi Patricia Chakur Brum Mayana Zatz |
| author_facet | Carlos R Bueno Júnior Lucas C Pantaleão Vanessa A Voltarelli Luiz Henrique M Bozi Patricia Chakur Brum Mayana Zatz |
| author_sort | Carlos R Bueno Júnior |
| collection | DOAJ |
| description | The present investigation was undertaken to test whether exercise training (ET) associated with AMPK/PPAR agonists (EM) would improve skeletal muscle function in mdx mice. These drugs have the potential to improve oxidative metabolism. This is of particular interest because oxidative muscle fibers are less affected in the course of the disease than glycolitic counterparts. Therefore, a cohort of 34 male congenic C57Bl/10J mdx mice included in this study was randomly assigned into four groups: vehicle solution (V), EM [AICAR (AMPK agonist, 50 mg/Kg-1.day-1, ip) and GW 1516 (PPARδ agonist, 2.5 mg/Kg-1.day-1, gavage)], ET (voluntary running on activity wheel) and EM+ET. Functional performance (grip meter and rotarod), aerobic capacity (running test), muscle histopathology, serum creatine kinase (CK), levels of ubiquitined proteins, oxidative metabolism protein expression (AMPK, PPAR, myoglobin and SCD) and intracellular calcium handling (DHPR, SERCA and NCX) protein expression were analyzed. Treatments started when the animals were two months old and were maintained for one month. A significant functional improvement (p<0.05) was observed in animals submitted to the combination of ET and EM. CK levels were decreased and the expression of proteins related to oxidative metabolism was increased in this group. There were no differences among the groups in the intracellular calcium handling protein expression. To our knowledge, this is the first study that tested the association of ET with EM in an experimental model of muscular dystrophy. Our results suggest that the association of ET and EM should be further tested as a potential therapeutic approach in muscular dystrophies. |
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| format | Article |
| id | doaj.art-6e0c0508862742d09e63883eba553e3e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T06:31:43Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6e0c0508862742d09e63883eba553e3e2022-12-21T19:50:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4569910.1371/journal.pone.0045699Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance.Carlos R Bueno JúniorLucas C PantaleãoVanessa A VoltarelliLuiz Henrique M BoziPatricia Chakur BrumMayana ZatzThe present investigation was undertaken to test whether exercise training (ET) associated with AMPK/PPAR agonists (EM) would improve skeletal muscle function in mdx mice. These drugs have the potential to improve oxidative metabolism. This is of particular interest because oxidative muscle fibers are less affected in the course of the disease than glycolitic counterparts. Therefore, a cohort of 34 male congenic C57Bl/10J mdx mice included in this study was randomly assigned into four groups: vehicle solution (V), EM [AICAR (AMPK agonist, 50 mg/Kg-1.day-1, ip) and GW 1516 (PPARδ agonist, 2.5 mg/Kg-1.day-1, gavage)], ET (voluntary running on activity wheel) and EM+ET. Functional performance (grip meter and rotarod), aerobic capacity (running test), muscle histopathology, serum creatine kinase (CK), levels of ubiquitined proteins, oxidative metabolism protein expression (AMPK, PPAR, myoglobin and SCD) and intracellular calcium handling (DHPR, SERCA and NCX) protein expression were analyzed. Treatments started when the animals were two months old and were maintained for one month. A significant functional improvement (p<0.05) was observed in animals submitted to the combination of ET and EM. CK levels were decreased and the expression of proteins related to oxidative metabolism was increased in this group. There were no differences among the groups in the intracellular calcium handling protein expression. To our knowledge, this is the first study that tested the association of ET with EM in an experimental model of muscular dystrophy. Our results suggest that the association of ET and EM should be further tested as a potential therapeutic approach in muscular dystrophies.http://europepmc.org/articles/PMC3448675?pdf=render |
| spellingShingle | Carlos R Bueno Júnior Lucas C Pantaleão Vanessa A Voltarelli Luiz Henrique M Bozi Patricia Chakur Brum Mayana Zatz Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. PLoS ONE |
| title | Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. |
| title_full | Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. |
| title_fullStr | Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. |
| title_full_unstemmed | Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. |
| title_short | Combined effect of AMPK/PPAR agonists and exercise training in mdx mice functional performance. |
| title_sort | combined effect of ampk ppar agonists and exercise training in mdx mice functional performance |
| url | http://europepmc.org/articles/PMC3448675?pdf=render |
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