Selecting 16S rRNA Primers for Microbiome Analysis in a Host–Microbe System: The Case of the Jellyfish <i>Rhopilema nomadica</i>

Selecting 16S rRNA Primers for Microbiome Analysis in a Host–Microbe System: The Case of the Jellyfish <i>Rhopilema nomadica</i>

Amplicon sequencing of the 16S rRNA gene is extensively used to characterize bacterial communities, including those living in association with eukaryotic hosts. Deciding which region of the 16S rRNA gene to analyze and selecting the appropriate PCR primers remains a major decision when initiating an...

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Bibliographic Details
Main Authors: Noga Barak, Eduard Fadeev, Vera Brekhman, Dikla Aharonovich, Tamar Lotan, Daniel Sher
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Microorganisms
Subjects:
cnidaria
jellyfish
amplicon sequencing
next-generation sequencing
microbial community
method comparison
Online Access:https://www.mdpi.com/2076-2607/11/4/955

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