Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function
Effective growth and replication of obligate intracellular pathogens depend on host cell metabolism. How this is connected to host cell mitochondrial function has not been studied so far. Recent studies suggest that growth of intracellular bacteria such as Chlamydia pneumoniae is enhanced in a low o...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00499/full |
| _version_ | 1818858019551707136 |
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| author | Nadja Käding Inga Kaufhold Constanze Müller Marta Szaszák Kensuke Shima Thomas Weinmaier Rodrigo Lomas Ana Conesa Ana Conesa Philippe Schmitt-Kopplin Thomas Rattei Jan Rupp |
| author_facet | Nadja Käding Inga Kaufhold Constanze Müller Marta Szaszák Kensuke Shima Thomas Weinmaier Rodrigo Lomas Ana Conesa Ana Conesa Philippe Schmitt-Kopplin Thomas Rattei Jan Rupp |
| author_sort | Nadja Käding |
| collection | DOAJ |
| description | Effective growth and replication of obligate intracellular pathogens depend on host cell metabolism. How this is connected to host cell mitochondrial function has not been studied so far. Recent studies suggest that growth of intracellular bacteria such as Chlamydia pneumoniae is enhanced in a low oxygen environment, arguing for a particular mechanistic role of the mitochondrial respiration in controlling intracellular progeny. Metabolic changes in C. pneumoniae infected epithelial cells were analyzed under normoxic (O2 ≈ 20%) and hypoxic conditions (O2 < 3%). We observed that infection of epithelial cells with C. pneumoniae under normoxia impaired mitochondrial function characterized by an enhanced mitochondrial membrane potential and ROS generation. Knockdown and mutation of the host cell ATP synthase resulted in an increased chlamydial replication already under normoxic conditions. As expected, mitochondrial hyperpolarization was observed in non-infected control cells cultured under hypoxic conditions, which was beneficial for C. pneumoniae growth. Taken together, functional and genetically encoded mitochondrial dysfunction strongly promotes intracellular growth of C. pneumoniae. |
| first_indexed | 2024-12-19T08:49:38Z |
| format | Article |
| id | doaj.art-6e0ed4767ad546bcace68e89dffbd46f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-19T08:49:38Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-6e0ed4767ad546bcace68e89dffbd46f2022-12-21T20:28:45ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-12-01710.3389/fcimb.2017.00499304483Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial FunctionNadja Käding0Inga Kaufhold1Constanze Müller2Marta Szaszák3Kensuke Shima4Thomas Weinmaier5Rodrigo Lomas6Ana Conesa7Ana Conesa8Philippe Schmitt-Kopplin9Thomas Rattei10Jan Rupp11Department of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, GermanyDepartment of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, GermanyResearch Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, GermanyDepartment of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, GermanyDepartment of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, GermanyDivision of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, AustriaGenomics of Gene Expression Lab, Centro de Investigaciones Príncipe Felipe, Valencia, SpainGenomics of Gene Expression Lab, Centro de Investigaciones Príncipe Felipe, Valencia, SpainMicrobiology and Cell Science, IFAS, University of Florida, Gainesville, FL, United StatesResearch Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, GermanyDivision of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, AustriaDepartment of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, GermanyEffective growth and replication of obligate intracellular pathogens depend on host cell metabolism. How this is connected to host cell mitochondrial function has not been studied so far. Recent studies suggest that growth of intracellular bacteria such as Chlamydia pneumoniae is enhanced in a low oxygen environment, arguing for a particular mechanistic role of the mitochondrial respiration in controlling intracellular progeny. Metabolic changes in C. pneumoniae infected epithelial cells were analyzed under normoxic (O2 ≈ 20%) and hypoxic conditions (O2 < 3%). We observed that infection of epithelial cells with C. pneumoniae under normoxia impaired mitochondrial function characterized by an enhanced mitochondrial membrane potential and ROS generation. Knockdown and mutation of the host cell ATP synthase resulted in an increased chlamydial replication already under normoxic conditions. As expected, mitochondrial hyperpolarization was observed in non-infected control cells cultured under hypoxic conditions, which was beneficial for C. pneumoniae growth. Taken together, functional and genetically encoded mitochondrial dysfunction strongly promotes intracellular growth of C. pneumoniae.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00499/fullmitochondriahypoxiaChlamydia pneumoniaemetabolismhost-pathogen interaction |
| spellingShingle | Nadja Käding Inga Kaufhold Constanze Müller Marta Szaszák Kensuke Shima Thomas Weinmaier Rodrigo Lomas Ana Conesa Ana Conesa Philippe Schmitt-Kopplin Thomas Rattei Jan Rupp Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function Frontiers in Cellular and Infection Microbiology mitochondria hypoxia Chlamydia pneumoniae metabolism host-pathogen interaction |
| title | Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function |
| title_full | Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function |
| title_fullStr | Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function |
| title_full_unstemmed | Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function |
| title_short | Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function |
| title_sort | growth of chlamydia pneumoniae is enhanced in cells with impaired mitochondrial function |
| topic | mitochondria hypoxia Chlamydia pneumoniae metabolism host-pathogen interaction |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00499/full |
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