Photoacoustic Properties of Polypyrrole Nanoparticles
Photoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/9/2457 |
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| author | Peter Keša Monika Paúrová Michal Babič Tomáš Heizer Petr Matouš Karolína Turnovcová Dana Mareková Luděk Šefc Vít Herynek |
| author_facet | Peter Keša Monika Paúrová Michal Babič Tomáš Heizer Petr Matouš Karolína Turnovcová Dana Mareková Luděk Šefc Vít Herynek |
| author_sort | Peter Keša |
| collection | DOAJ |
| description | Photoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypyrrole nanoparticles by water-based redox precipitation polymerization in the presence of ammonium persulphate (ratio <i>n</i>Py:<i>n</i>Oxi 1:0.5, 1:1, 1:2, 1:3, 1:5) or iron(III) chloride (<i>n</i>Py:<i>n</i>Oxi 1:2.3) acting as an oxidant. To stabilize growing nanoparticles, non-ionic polyvinylpyrrolidone was used. The nanoparticles were characterized and tested as a photoacoustic contrast agent in vitro on an imaging platform combining ultrasound and photoacoustic imaging. High photoacoustic signals were obtained with lower ratios of the oxidant (<i>n</i>Py:<i>n</i>APS ≥ 1:2), which corresponded to higher number of conjugated bonds in the polymer. The increasing portion of oxidized structures probably shifted the absorption spectra towards shorter wavelengths. A strong photoacoustic signal dependence on the nanoparticle size was revealed; the signal linearly increased with particle surface. Coated nanoparticles were also tested in vivo on a mouse model. To conclude, polypyrrole nanoparticles represent a promising contrast agent for photoacoustic imaging. Variations in the preparation result in varying photoacoustic properties related to their structure and allow to optimize the nanoparticles for in vivo imaging. |
| first_indexed | 2024-03-10T07:22:10Z |
| format | Article |
| id | doaj.art-6e0fb7a42ff041d59f13e096be46dc33 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T07:22:10Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-6e0fb7a42ff041d59f13e096be46dc332023-11-22T14:32:53ZengMDPI AGNanomaterials2079-49912021-09-01119245710.3390/nano11092457Photoacoustic Properties of Polypyrrole NanoparticlesPeter Keša0Monika Paúrová1Michal Babič2Tomáš Heizer3Petr Matouš4Karolína Turnovcová5Dana Mareková6Luděk Šefc7Vít Herynek8Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, 120 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Science, 162 06 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Science, 162 06 Prague, Czech RepublicCenter for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, 120 00 Prague, Czech RepublicCenter for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, 120 00 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Science, 142 20 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Science, 142 20 Prague, Czech RepublicCenter for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, 120 00 Prague, Czech RepublicCenter for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, 120 00 Prague, Czech RepublicPhotoacoustic imaging, an emerging modality, provides supplemental information to ultrasound imaging. We investigated the properties of polypyrrole nanoparticles, which considerably enhance contrast in photoacoustic images, in relation to the synthesis procedure and to their size. We prepared polypyrrole nanoparticles by water-based redox precipitation polymerization in the presence of ammonium persulphate (ratio <i>n</i>Py:<i>n</i>Oxi 1:0.5, 1:1, 1:2, 1:3, 1:5) or iron(III) chloride (<i>n</i>Py:<i>n</i>Oxi 1:2.3) acting as an oxidant. To stabilize growing nanoparticles, non-ionic polyvinylpyrrolidone was used. The nanoparticles were characterized and tested as a photoacoustic contrast agent in vitro on an imaging platform combining ultrasound and photoacoustic imaging. High photoacoustic signals were obtained with lower ratios of the oxidant (<i>n</i>Py:<i>n</i>APS ≥ 1:2), which corresponded to higher number of conjugated bonds in the polymer. The increasing portion of oxidized structures probably shifted the absorption spectra towards shorter wavelengths. A strong photoacoustic signal dependence on the nanoparticle size was revealed; the signal linearly increased with particle surface. Coated nanoparticles were also tested in vivo on a mouse model. To conclude, polypyrrole nanoparticles represent a promising contrast agent for photoacoustic imaging. Variations in the preparation result in varying photoacoustic properties related to their structure and allow to optimize the nanoparticles for in vivo imaging.https://www.mdpi.com/2079-4991/11/9/2457photoacoustic imagingpolypyrrolenanoparticlescontrast agents |
| spellingShingle | Peter Keša Monika Paúrová Michal Babič Tomáš Heizer Petr Matouš Karolína Turnovcová Dana Mareková Luděk Šefc Vít Herynek Photoacoustic Properties of Polypyrrole Nanoparticles Nanomaterials photoacoustic imaging polypyrrole nanoparticles contrast agents |
| title | Photoacoustic Properties of Polypyrrole Nanoparticles |
| title_full | Photoacoustic Properties of Polypyrrole Nanoparticles |
| title_fullStr | Photoacoustic Properties of Polypyrrole Nanoparticles |
| title_full_unstemmed | Photoacoustic Properties of Polypyrrole Nanoparticles |
| title_short | Photoacoustic Properties of Polypyrrole Nanoparticles |
| title_sort | photoacoustic properties of polypyrrole nanoparticles |
| topic | photoacoustic imaging polypyrrole nanoparticles contrast agents |
| url | https://www.mdpi.com/2079-4991/11/9/2457 |
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