The Role of Vibrio cholerae Haemagglutinin Protease (HAP) in Extra-Intestinal Infection
Introduction: Based on the diversity of surface O antigen Vibrio cholerae can be classified into 206 serogroups. Vibrio cholerae is the causative agent of cholera and extra intestinal infections like, septicemia, wound infection and haemorrhagic reactions. Pathogenic factors of V. cholerae extra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8461/16510_CE(RA1)_F(T)_PF1(ROESAK)_PFA(AK).pdf |
| Summary: | Introduction: Based on the diversity of surface O antigen Vibrio
cholerae can be classified into 206 serogroups. Vibrio cholerae
is the causative agent of cholera and extra intestinal infections
like, septicemia, wound infection and haemorrhagic reactions.
Pathogenic factors of V. cholerae extra-intestinal infection are yet
to be explored.
Aim: To identify the pathogenic factor associated with V. cholerae
extra-intestinal infection.
Materials and Methods: This study was carried out between
April, 2007 to October 2007 in National Institute of Cholera and
Enteric Diseases (NICED). Haemagglutinin Protease (HAP), a
major secreted proteolytic enzyme, was purified from the culture
supernatant of Vibrio cholerae O1 strain C6709 after removal
of outer membrane vesicles using a single step ion-exchange
chromatography. Function of HAP was characterized by animal
model, like, subcutaneous mouse assay, basement membrane
component’s degradation assays and tissue culture assays.
Results: When suckling mouse was subcutaneously injected with
culture supernatant of C6709 strain or purified HAP in both cases,
distinct in vivo haemorrhagic response along with histopathological
changes like necrosis of the capillaries and muscle layer, acute
myofibre degeneration as well as moderate number of erythrocyte
scattered through the skin, capillary necrosis, acute myofiber
degeneration and necrosis of muscle layer were found. When
Tryptic Soy Broth (TSB) media was used, the haemorrhagic
effects in suckling mouse were not detectable. The major protein
components, laminin and collagen, of basement membrane
comprising of vascular endothelial cells, were degraded by HAP.
Purified HAP showed cell rounding effects on Int 407 cells.
Conclusion: Result indicates that HAP may be a causative agent
of Vibrio cholerae mediated extra-intestinal infection. This study
confirms that Vibrio cholera as a sole pathogen can cause the
extra-intestinal infection. This information is important for public
health notification. Besides this, result indicates appropriate
testing for Vibrio cholerae and intervention are important for the
patient management. |
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| ISSN: | 2249-782X 0973-709X |