Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
Penicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on mu...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-07-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/13/8/4733 |
| _version_ | 1798042251724587008 |
|---|---|
| author | Qin-Ying Liu Tong Zhou Yang-Yang Zhao Li Chen Mei-Wei Gong Qi-Wen Xia Min-Gang Ying Qiu-Hong Zheng Qi-Qing Zhang |
| author_facet | Qin-Ying Liu Tong Zhou Yang-Yang Zhao Li Chen Mei-Wei Gong Qi-Wen Xia Min-Gang Ying Qiu-Hong Zheng Qi-Qing Zhang |
| author_sort | Qin-Ying Liu |
| collection | DOAJ |
| description | Penicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on multiple tumor types. Among them, the human malignant melanoma cell A-375 was confirmed to be the most sensitive. Morphologic evaluation, apoptosis rate analysis, Western blot and real-time quantitative PCR (RT-qPCR) results showed penicitrinine A could significantly induce A-375 cell apoptosis by decreasing the expression of Bcl-2 and increasing the expression of Bax. Moreover, we investigated the anti-metastatic effects of penicitrinine A in A-375 cells by wound healing assay, trans-well assay, Western blot and RT-qPCR. The results showed penicitrinine A significantly suppressed metastatic activity of A-375 cells by regulating the expression of MMP-9 and its specific inhibitor TIMP-1. These findings suggested that penicitrinine A might serve as a potential antitumor agent, which could inhibit the proliferation and metastasis of tumor cells. |
| first_indexed | 2024-04-11T22:32:54Z |
| format | Article |
| id | doaj.art-6e12157342a74b878be3e69b4bac93ce |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:32:54Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6e12157342a74b878be3e69b4bac93ce2022-12-22T03:59:18ZengMDPI AGMarine Drugs1660-33972015-07-011384733475310.3390/md13084733md13084733Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinumQin-Ying Liu0Tong Zhou1Yang-Yang Zhao2Li Chen3Mei-Wei Gong4Qi-Wen Xia5Min-Gang Ying6Qiu-Hong Zheng7Qi-Qing Zhang8Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Tumor Hospital, Fuzhou 350014, ChinaFujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Tumor Hospital, Fuzhou 350014, ChinaInstitute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, ChinaPenicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on multiple tumor types. Among them, the human malignant melanoma cell A-375 was confirmed to be the most sensitive. Morphologic evaluation, apoptosis rate analysis, Western blot and real-time quantitative PCR (RT-qPCR) results showed penicitrinine A could significantly induce A-375 cell apoptosis by decreasing the expression of Bcl-2 and increasing the expression of Bax. Moreover, we investigated the anti-metastatic effects of penicitrinine A in A-375 cells by wound healing assay, trans-well assay, Western blot and RT-qPCR. The results showed penicitrinine A significantly suppressed metastatic activity of A-375 cells by regulating the expression of MMP-9 and its specific inhibitor TIMP-1. These findings suggested that penicitrinine A might serve as a potential antitumor agent, which could inhibit the proliferation and metastasis of tumor cells.http://www.mdpi.com/1660-3397/13/8/4733penicitrinine Amarine-derived fungushuman malignant melanoma cell A-375anticancer activityapoptosisanti-metastatic |
| spellingShingle | Qin-Ying Liu Tong Zhou Yang-Yang Zhao Li Chen Mei-Wei Gong Qi-Wen Xia Min-Gang Ying Qiu-Hong Zheng Qi-Qing Zhang Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum Marine Drugs penicitrinine A marine-derived fungus human malignant melanoma cell A-375 anticancer activity apoptosis anti-metastatic |
| title | Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum |
| title_full | Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum |
| title_fullStr | Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum |
| title_full_unstemmed | Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum |
| title_short | Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum |
| title_sort | antitumor effects and related mechanisms of penicitrinine a a novel alkaloid with a unique spiro skeleton from the marine fungus penicillium citrinum |
| topic | penicitrinine A marine-derived fungus human malignant melanoma cell A-375 anticancer activity apoptosis anti-metastatic |
| url | http://www.mdpi.com/1660-3397/13/8/4733 |
| work_keys_str_mv | AT qinyingliu antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT tongzhou antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT yangyangzhao antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT lichen antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT meiweigong antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT qiwenxia antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT mingangying antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT qiuhongzheng antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum AT qiqingzhang antitumoreffectsandrelatedmechanismsofpenicitrinineaanovelalkaloidwithauniquespiroskeletonfromthemarinefunguspenicilliumcitrinum |