The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet
DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verifie...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/1118 |
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| author | Jiamin Weng Hui Wang Dayou Cheng Tianjiao Liu Deyong Zeng Cuihong Dai Chengfei Luo |
| author_facet | Jiamin Weng Hui Wang Dayou Cheng Tianjiao Liu Deyong Zeng Cuihong Dai Chengfei Luo |
| author_sort | Jiamin Weng |
| collection | DOAJ |
| description | DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verified by bisulfite transformation, indicating that the results of WGBS technology were reliable. In addition, 12 differential methylation genes (DMGs) were identified, which were related to carbohydrate and energy metabolism, pollen wall development, and endogenous hormone regulation. Quantitative real-time PCR (qRT-PCR) showed that 75% of DMG expression levels showed negative feedback with methylation level, indicating that DNA methylation can affect gene expression to a certain extent. In addition, we found hypermethylation inhibited gene expression, which laid a foundation for further study on the molecular mechanism of DNA methylation at the epigenetic level in sugar beet male sterility. |
| first_indexed | 2024-03-08T09:52:52Z |
| format | Article |
| id | doaj.art-6e12eb128168469c8b1bf010d3426905 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:52:52Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6e12eb128168469c8b1bf010d34269052024-01-29T13:57:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252111810.3390/ijms25021118The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar BeetJiamin Weng0Hui Wang1Dayou Cheng2Tianjiao Liu3Deyong Zeng4Cuihong Dai5Chengfei Luo6School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150001, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, ChinaDNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verified by bisulfite transformation, indicating that the results of WGBS technology were reliable. In addition, 12 differential methylation genes (DMGs) were identified, which were related to carbohydrate and energy metabolism, pollen wall development, and endogenous hormone regulation. Quantitative real-time PCR (qRT-PCR) showed that 75% of DMG expression levels showed negative feedback with methylation level, indicating that DNA methylation can affect gene expression to a certain extent. In addition, we found hypermethylation inhibited gene expression, which laid a foundation for further study on the molecular mechanism of DNA methylation at the epigenetic level in sugar beet male sterility.https://www.mdpi.com/1422-0067/25/2/1118sugar beetcytoplasmic male sterilityDNA methylationwhole-genome bisulfite sequencingmethylated gene |
| spellingShingle | Jiamin Weng Hui Wang Dayou Cheng Tianjiao Liu Deyong Zeng Cuihong Dai Chengfei Luo The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet International Journal of Molecular Sciences sugar beet cytoplasmic male sterility DNA methylation whole-genome bisulfite sequencing methylated gene |
| title | The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet |
| title_full | The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet |
| title_fullStr | The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet |
| title_full_unstemmed | The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet |
| title_short | The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet |
| title_sort | effects of dna methylation on cytoplasmic male sterility in sugar beet |
| topic | sugar beet cytoplasmic male sterility DNA methylation whole-genome bisulfite sequencing methylated gene |
| url | https://www.mdpi.com/1422-0067/25/2/1118 |
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