Proteomic profile of tepary bean seed storage proteins in germination with low water potential
Abstract Background Tepary bean (Phaseolus acutifolius A. Gray) is one of the five species domesticated from the genus Phaseolus with genetic resistance to biotic and abiotic stress. To understand the mechanisms underlying drought responses in seed storage proteins germinated on water and polyethyle...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Proteome Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12953-023-00225-6 |
| _version_ | 1797355987484540928 |
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| author | Daniel Padilla-Chacón Laura Campos-Patiño Cecilia B. Peña-Valdivia Antonio García-Esteva José Cruz Jiménez-Galindo Jorge Luis Pizeno-García |
| author_facet | Daniel Padilla-Chacón Laura Campos-Patiño Cecilia B. Peña-Valdivia Antonio García-Esteva José Cruz Jiménez-Galindo Jorge Luis Pizeno-García |
| author_sort | Daniel Padilla-Chacón |
| collection | DOAJ |
| description | Abstract Background Tepary bean (Phaseolus acutifolius A. Gray) is one of the five species domesticated from the genus Phaseolus with genetic resistance to biotic and abiotic stress. To understand the mechanisms underlying drought responses in seed storage proteins germinated on water and polyethylene glycol (PEG-6000) at -0.49 MPa, we used a proteomics approach to identify potential molecular target proteins associated with the low water potential stress response. Methods Storage proteins from cotyledons of Tepary bean seeds germinated at 24, 48 and 72 h on water and PEG-6000 at -0.49 MPa were analyzed by one-dimensional electrophoresis (DE) with 2-DE analysis and shotgun mass spectrometry. Using computational database searching and bioinformatics analyses, we performed Gene Ontology (GO) and protein interactome (functional protein association network) String analyses. Results Comparative analysis showed that the effect of PEG-6000 on root growth was parallel to that on germination. Based on the SDS‒PAGE protein banding patterns and 2-DE analysis, ten differentially abundant seed storage proteins showed changes in storage proteins, principally in the phaseolin and lectin fractions. We found many proteins that are recognized as drought stress-responsive proteins, and several of them are predicted to be intrinsically related to abiotic stress. The shotgun analysis searched against UniProt’s legume database, and Gene Ontology (GO) analysis indicated that most of the seed proteins were cytosolic, with catalytic activity and associated with carbohydrate metabolism. The protein‒protein interaction networks from functional enrichment analysis showed that phytohemagglutinin interacts with proteins associated with the degradation of storage proteins in the cotyledons of common bean during germination. Conclusion These findings suggest that Tepary bean seed proteins provide valuable information with the potential to be used in genetic improvement and are part of the drought stress response, making our approach a potentially useful strategy for discovering novel drought-responsive proteins in other plant models. |
| first_indexed | 2024-03-08T14:19:07Z |
| format | Article |
| id | doaj.art-6e13b6da7b8d4d4bb42de942c8645bd8 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-03-08T14:19:07Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-6e13b6da7b8d4d4bb42de942c8645bd82024-01-14T12:12:25ZengBMCProteome Science1477-59562024-01-0122111210.1186/s12953-023-00225-6Proteomic profile of tepary bean seed storage proteins in germination with low water potentialDaniel Padilla-Chacón0Laura Campos-Patiño1Cecilia B. Peña-Valdivia2Antonio García-Esteva3José Cruz Jiménez-Galindo4Jorge Luis Pizeno-García5Colegio de Postgraduados, CONAHCYT-Programa de Posgrado en BotánicaPrograma de Posgrado en Botánica, Colegio de PostgraduadosPrograma de Posgrado en Botánica, Colegio de PostgraduadosPrograma de Posgrado en Botánica, Colegio de PostgraduadosCampo Experimental Sierra de Chihuahua-INIFAPPrograma de Posgrado en Botánica, Colegio de PostgraduadosAbstract Background Tepary bean (Phaseolus acutifolius A. Gray) is one of the five species domesticated from the genus Phaseolus with genetic resistance to biotic and abiotic stress. To understand the mechanisms underlying drought responses in seed storage proteins germinated on water and polyethylene glycol (PEG-6000) at -0.49 MPa, we used a proteomics approach to identify potential molecular target proteins associated with the low water potential stress response. Methods Storage proteins from cotyledons of Tepary bean seeds germinated at 24, 48 and 72 h on water and PEG-6000 at -0.49 MPa were analyzed by one-dimensional electrophoresis (DE) with 2-DE analysis and shotgun mass spectrometry. Using computational database searching and bioinformatics analyses, we performed Gene Ontology (GO) and protein interactome (functional protein association network) String analyses. Results Comparative analysis showed that the effect of PEG-6000 on root growth was parallel to that on germination. Based on the SDS‒PAGE protein banding patterns and 2-DE analysis, ten differentially abundant seed storage proteins showed changes in storage proteins, principally in the phaseolin and lectin fractions. We found many proteins that are recognized as drought stress-responsive proteins, and several of them are predicted to be intrinsically related to abiotic stress. The shotgun analysis searched against UniProt’s legume database, and Gene Ontology (GO) analysis indicated that most of the seed proteins were cytosolic, with catalytic activity and associated with carbohydrate metabolism. The protein‒protein interaction networks from functional enrichment analysis showed that phytohemagglutinin interacts with proteins associated with the degradation of storage proteins in the cotyledons of common bean during germination. Conclusion These findings suggest that Tepary bean seed proteins provide valuable information with the potential to be used in genetic improvement and are part of the drought stress response, making our approach a potentially useful strategy for discovering novel drought-responsive proteins in other plant models.https://doi.org/10.1186/s12953-023-00225-6Tepary beanGerminationSeed storage proteinLow water potentialProteomics |
| spellingShingle | Daniel Padilla-Chacón Laura Campos-Patiño Cecilia B. Peña-Valdivia Antonio García-Esteva José Cruz Jiménez-Galindo Jorge Luis Pizeno-García Proteomic profile of tepary bean seed storage proteins in germination with low water potential Proteome Science Tepary bean Germination Seed storage protein Low water potential Proteomics |
| title | Proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| title_full | Proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| title_fullStr | Proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| title_full_unstemmed | Proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| title_short | Proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| title_sort | proteomic profile of tepary bean seed storage proteins in germination with low water potential |
| topic | Tepary bean Germination Seed storage protein Low water potential Proteomics |
| url | https://doi.org/10.1186/s12953-023-00225-6 |
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