Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples
Background: A micellar electrokinetic chromatographic (MEKC)/ indirect UV detection method with hydrodynamic and electrokinetic injection has been developed for the determination of pregabalin in the serum samples. Methods: Separation of the drug was achieved on Agilent capillary electrophorese in l...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2018-12-01
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| Series: | Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://ps.tbzmed.ac.ir/PDF/PHARM_1041_20180608182406 |
| _version_ | 1818433120962084864 |
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| author | Hamid Reza Sargazi Elnaz Tamizi Elaheh Rahimpour Abolghasem Jouyban |
| author_facet | Hamid Reza Sargazi Elnaz Tamizi Elaheh Rahimpour Abolghasem Jouyban |
| author_sort | Hamid Reza Sargazi |
| collection | DOAJ |
| description | Background: A micellar electrokinetic chromatographic (MEKC)/ indirect UV detection method with hydrodynamic and electrokinetic injection has been developed for the determination of pregabalin in the serum samples. Methods: Separation of the drug was achieved on Agilent capillary electrophorese in less than 5 min using a 50 cm × 75 μm i.d. uncoated fused-silica capillary and a background electrolyte (BGE) consisting of 5-aminosalicylic acid (5-ASA, 10 mmol L-1), cetyl trimethylammonium bromide (CTAB, 1 mmol L-1) and tri-sodium citrate (4% w/v). The influence of various parameters on the separation such as separation voltage, injection time, cassette temperature, pH of BGE and organic modifier was investigated. Results: Method validation shown good linearity (R2> 0.999) in the range of 1.5-100 µg mL-1 of pregabalin. A limit of detection (LOD) of 0.8 μg mL-1 and a limit of quantitation (LOQ) of 2.6 μg mL-1 were reported for pregabalin. Conclusion: The proposed method was found to be suitable and accurate for the determination of pregabalin in serum samples. |
| first_indexed | 2024-12-14T16:16:03Z |
| format | Article |
| id | doaj.art-6e144c9833574d12acb7e64645ed493b |
| institution | Directory Open Access Journal |
| issn | 1735-403X 2383-2886 |
| language | English |
| last_indexed | 2024-12-14T16:16:03Z |
| publishDate | 2018-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-6e144c9833574d12acb7e64645ed493b2022-12-21T22:54:54ZengTabriz University of Medical SciencesPharmaceutical Sciences1735-403X2383-28862018-12-0124429830310.15171/PS.2018.43PHARM_1041_20180608182406Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum SamplesHamid Reza Sargazi0Elnaz Tamizi1Elaheh Rahimpour2Abolghasem Jouyban3Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Pharmaceutical Analysis Research Center, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.Background: A micellar electrokinetic chromatographic (MEKC)/ indirect UV detection method with hydrodynamic and electrokinetic injection has been developed for the determination of pregabalin in the serum samples. Methods: Separation of the drug was achieved on Agilent capillary electrophorese in less than 5 min using a 50 cm × 75 μm i.d. uncoated fused-silica capillary and a background electrolyte (BGE) consisting of 5-aminosalicylic acid (5-ASA, 10 mmol L-1), cetyl trimethylammonium bromide (CTAB, 1 mmol L-1) and tri-sodium citrate (4% w/v). The influence of various parameters on the separation such as separation voltage, injection time, cassette temperature, pH of BGE and organic modifier was investigated. Results: Method validation shown good linearity (R2> 0.999) in the range of 1.5-100 µg mL-1 of pregabalin. A limit of detection (LOD) of 0.8 μg mL-1 and a limit of quantitation (LOQ) of 2.6 μg mL-1 were reported for pregabalin. Conclusion: The proposed method was found to be suitable and accurate for the determination of pregabalin in serum samples.https://ps.tbzmed.ac.ir/PDF/PHARM_1041_20180608182406Field-amplified sample injectionMicellar electrokinetic chromatographyCapillary electrophoresisPregabalin |
| spellingShingle | Hamid Reza Sargazi Elnaz Tamizi Elaheh Rahimpour Abolghasem Jouyban Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples Pharmaceutical Sciences Field-amplified sample injection Micellar electrokinetic chromatography Capillary electrophoresis Pregabalin |
| title | Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples |
| title_full | Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples |
| title_fullStr | Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples |
| title_full_unstemmed | Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples |
| title_short | Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples |
| title_sort | development of a micellar electrokinetic chromatographic method with indirect uv detection for pregabalin determination in serum samples |
| topic | Field-amplified sample injection Micellar electrokinetic chromatography Capillary electrophoresis Pregabalin |
| url | https://ps.tbzmed.ac.ir/PDF/PHARM_1041_20180608182406 |
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