MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9....
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2016-12-01
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| Series: | Journal of Engineering Studies and Research |
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| Online Access: | https://jesr.ub.ro/1/article/view/92 |
| _version_ | 1797202254370963456 |
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| author | AHMED JASSIM MUKLIVE AL-OGAIDI |
| author_facet | AHMED JASSIM MUKLIVE AL-OGAIDI |
| author_sort | AHMED JASSIM MUKLIVE AL-OGAIDI |
| collection | DOAJ |
| description |
The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9.14x10-5 – 2.28x10-3 μg.mL-1) was realized and the value of molar absortivity was 1.5x103 L.mol-1.cm-1, with a relative standard deviation more than 1.26% and a recovery 98.77%. As we study the nature of the azo dye by mole fraction method, from the practical value we found that the mole fraction of the dye compound is 1:1 (dopamine: 4- amino antipyrine) and the stability factor reach to 1.4x106 L.mol-1. The described procedure is very simple, low-time-consuming, provides high throughput of examined samples, and could be used for routine screening and confirmatory analyses as well. The method was successfully validated to the analysis of the dopamine in biological samples.
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| first_indexed | 2024-03-07T17:27:10Z |
| format | Article |
| id | doaj.art-6e17f9976b0843a6aee1af1fe2372f89 |
| institution | Directory Open Access Journal |
| issn | 2068-7559 2344-4932 |
| language | English |
| last_indexed | 2024-04-24T08:00:31Z |
| publishDate | 2016-12-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Journal of Engineering Studies and Research |
| spelling | doaj.art-6e17f9976b0843a6aee1af1fe2372f892024-04-17T19:35:48ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauJournal of Engineering Studies and Research2068-75592344-49322016-12-01224MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTIONAHMED JASSIM MUKLIVE AL-OGAIDI 0University of Technology, Department of applied Sciences, Applied Chemistry section. Iraq, Baghdad The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9.14x10-5 – 2.28x10-3 μg.mL-1) was realized and the value of molar absortivity was 1.5x103 L.mol-1.cm-1, with a relative standard deviation more than 1.26% and a recovery 98.77%. As we study the nature of the azo dye by mole fraction method, from the practical value we found that the mole fraction of the dye compound is 1:1 (dopamine: 4- amino antipyrine) and the stability factor reach to 1.4x106 L.mol-1. The described procedure is very simple, low-time-consuming, provides high throughput of examined samples, and could be used for routine screening and confirmatory analyses as well. The method was successfully validated to the analysis of the dopamine in biological samples. https://jesr.ub.ro/1/article/view/92dopaminecoupling reactionbiological samplesazo dyesspectrophotometric |
| spellingShingle | AHMED JASSIM MUKLIVE AL-OGAIDI MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION Journal of Engineering Studies and Research dopamine coupling reaction biological samples azo dyes spectrophotometric |
| title | MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION |
| title_full | MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION |
| title_fullStr | MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION |
| title_full_unstemmed | MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION |
| title_short | MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION |
| title_sort | micro determination of dopamine in biological samples by coupling reaction |
| topic | dopamine coupling reaction biological samples azo dyes spectrophotometric |
| url | https://jesr.ub.ro/1/article/view/92 |
| work_keys_str_mv | AT ahmedjassimmuklivealogaidi microdeterminationofdopamineinbiologicalsamplesbycouplingreaction |