Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora
Lycopene cyclase needs to be inhibited by the blockers like pyridine or imidazole in the lycopene accumulation of Blakeslea trispora. This work investigated how pyridine and imidazole impacted the basal metabolism of B. trispora, the results helped us understand how they could affect the lycopene pr...
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00347/full |
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| author | Yang Liu Yang Liu Yang Liu Xiang-yu Li Shu-huan Lu Chao Yu Yu-zhou Zhang Yu-zhou Zhang Zhi-ming Wang Jian-ming Yao Jian-ming Yao |
| author_facet | Yang Liu Yang Liu Yang Liu Xiang-yu Li Shu-huan Lu Chao Yu Yu-zhou Zhang Yu-zhou Zhang Zhi-ming Wang Jian-ming Yao Jian-ming Yao |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Lycopene cyclase needs to be inhibited by the blockers like pyridine or imidazole in the lycopene accumulation of Blakeslea trispora. This work investigated how pyridine and imidazole impacted the basal metabolism of B. trispora, the results helped us understand how they could affect the lycopene production and application, and see the metabolic risks from different inhibitors. In this study, the highest yield of lycopene with pyridine was obtained at 176 mg/L without amino acids supplement, and got more lycopene at 237 mg/L adding tyrosine, lysine, proline all together as 0.01 mol/L each in fermented broth. GC-MS and Principal Component Analysis (PCA) were used to find that amino acids, fatty acids, organic acids including phosphoric acid, carbon source and imidazole derivatives played the most important roles in lycopene fermentation with imidazole, differently, fatty acids, carbon source, and pyridine derivatives were more significant in the pyridine process and it was remarkable that the residual of both blockers' derivatives would bring the potential risks on applications of lycopene products. Predominantly, durene met 0.35 mg/g DCW with imidazole and piperidine formaldehyde attained 0.24 mg/g DCW with pyridine after the end of lycopene fermentations. |
| first_indexed | 2024-12-11T09:51:19Z |
| format | Article |
| id | doaj.art-6e1a32b8ad09481999715a4c7c0ae229 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T09:51:19Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-6e1a32b8ad09481999715a4c7c0ae2292022-12-22T01:12:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-11-01710.3389/fbioe.2019.00347490421Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trisporaYang Liu0Yang Liu1Yang Liu2Xiang-yu Li3Shu-huan Lu4Chao Yu5Yu-zhou Zhang6Yu-zhou Zhang7Zhi-ming Wang8Jian-ming Yao9Jian-ming Yao10Biotechnology Center, Institute of Plasma Physics, and Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaUniversity of Science and Technology of China, Hefei, ChinaCABIO Biotech (Wuhan) Co. Ltd., Wuhan, ChinaCABIO Biotech (Wuhan) Co. Ltd., Wuhan, ChinaCABIO Biotech (Wuhan) Co. Ltd., Wuhan, ChinaCABIO Biotech (Wuhan) Co. Ltd., Wuhan, ChinaBiotechnology Center, Institute of Plasma Physics, and Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaUniversity of Science and Technology of China, Hefei, ChinaCABIO Biotech (Wuhan) Co. Ltd., Wuhan, ChinaBiotechnology Center, Institute of Plasma Physics, and Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, ChinaUniversity of Science and Technology of China, Hefei, ChinaLycopene cyclase needs to be inhibited by the blockers like pyridine or imidazole in the lycopene accumulation of Blakeslea trispora. This work investigated how pyridine and imidazole impacted the basal metabolism of B. trispora, the results helped us understand how they could affect the lycopene production and application, and see the metabolic risks from different inhibitors. In this study, the highest yield of lycopene with pyridine was obtained at 176 mg/L without amino acids supplement, and got more lycopene at 237 mg/L adding tyrosine, lysine, proline all together as 0.01 mol/L each in fermented broth. GC-MS and Principal Component Analysis (PCA) were used to find that amino acids, fatty acids, organic acids including phosphoric acid, carbon source and imidazole derivatives played the most important roles in lycopene fermentation with imidazole, differently, fatty acids, carbon source, and pyridine derivatives were more significant in the pyridine process and it was remarkable that the residual of both blockers' derivatives would bring the potential risks on applications of lycopene products. Predominantly, durene met 0.35 mg/g DCW with imidazole and piperidine formaldehyde attained 0.24 mg/g DCW with pyridine after the end of lycopene fermentations.https://www.frontiersin.org/article/10.3389/fbioe.2019.00347/fullmetabolismlycopenepyridineimidazoleBlakeslea trispora |
| spellingShingle | Yang Liu Yang Liu Yang Liu Xiang-yu Li Shu-huan Lu Chao Yu Yu-zhou Zhang Yu-zhou Zhang Zhi-ming Wang Jian-ming Yao Jian-ming Yao Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora Frontiers in Bioengineering and Biotechnology metabolism lycopene pyridine imidazole Blakeslea trispora |
| title | Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora |
| title_full | Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora |
| title_fullStr | Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora |
| title_full_unstemmed | Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora |
| title_short | Comparative Metabolic Responses Induced by Pyridine and Imidazole in Blakeslea trispora |
| title_sort | comparative metabolic responses induced by pyridine and imidazole in blakeslea trispora |
| topic | metabolism lycopene pyridine imidazole Blakeslea trispora |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00347/full |
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